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Archive of SID

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Archive of SID

39

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(6)

Archive of SID

41

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(7)

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(8)

Archive of SID

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j3 1- RastogiN; E. Legrand, et al. (2001). The

mycobacteria: an introduction to nomenclature and pathogenesis. Rev.Sci. Tech. 20(1): 21-54.

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3- Mosavari N; Jamshidian M; Seifiabadi Shahpouri M; Feizabadi M; Soleimani D;

Aref Pajouhi R; Soleimani K; Keshavarz R;

Taheri M; Shakibamehr A; Dashtipour S, (2009). Molecular genotyping and epidemiology of the most frequent Mycobacterium bovis strains obtained from cattle, Iran. Fifth International M.bovis Conference 25-28.August-Wellington,New Zealand.

4- Van Soolingen D; de Haas PEW; Haagsma J;

Eger T; Hermans PWM; Ritacco V; (1994).

Use of various genetic markers in differentiation of Mycobacterium bovis strains from animals and humans and for studying epidemiology of bovine tuberculosis. J. Clin. Microbiol. 32(10):

2425–2433.

5- Jacqueline Inwald, J. H., 2 Si Palmer,1 James Dale,1 Philip D. Butcher,2 R.

Glyn Hewinson,1 and a. S. V. Gordon (2003). "Genomic Analysis of Mycobacterium tuberculosis Complex Strains Used for Production of Purified Protein Derivative." JOURNAL OF CLINICAL MICROBIOLOGY, 3929– 3932.

6- M. Carmen Garcia Pelayo; J. N. G. Paul Golby; Christopher Pirson; Katie Ewer;

Martin Vordermeier R; Glyn Hewinson;

Stephen V; Gordon, ( 2009). Gene expression profiling and antigen mining of the tuberculin production strain Mycobacterium bovis AN5. Veterinary Microbiology; 133: 272–277.

7- Vuorinen P;. Miettinen A, (1995). Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test and Roche Amplicor Mycobacterium Tuberculosis Test. J. Clin. Microbiol. 33(7): 1856-9. 8- Van Soolingen D; P. E. de Haas; (2002).

Restriction fragment length

polymorphism (RFLP) typing of mycobacteria. National Instiute of Public Health and Environment,Bilthoven, The Netherlands.

9- Cousins D. V., R. A; Skuce,(1998).

Towards a standardized approach to DNA fingerprinting of Mycobacterium bovis. International Union Against Tuberculosis and Lung Disease, Tuberculosis in Animals Subsection. Int.

J .Tuberc. Lung. Dis. 2(6): 471-8. 10

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11- Epizooties O. I. D, (2004) Manual of standards for Diagnostic Tests and Vaccine.

12- Van Soolingen D; T. Hoogenboezem, (1997). A novel pathogenic taxon of the Mycobacterium tuberculosis complex, Canetti: characterization of an exceptional isolate from Africa Int. J.

Syst. Bacteriol. 47(4): 1236-45.

13- Stahl. D.A., U. J. W, (1990). The division between fast and slow growing species corresponds to natural relationships among the mycobacteria.

Journal of Bacteriology; 172: 116-124. 14- Cousins D. S; Williams, (1998).

Evaluation of four DNA typing techniques in epidemiological investigations of bovine tuberculosis. J.

Clin. Microbiol. 36(1): 168-78.

15- Tweedle N. E. L. P,( 1994). Bovine tuberculiosis control and eradication programs in Australia and NewZealand.

Veterinary Microbiology; 40: 23-39. 16- Groenen P.M.A; van Soolingen D; van

Embden J.D.A, (1993). Nature of DNA polymorphism in the direct repeat cluster of Mycobactetium tuberculosis;

application for strain differentiation by a novel typing method. Molecullar Microbiology; 10: 2953-2956.

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