) -(
! "
! " #
$% & '
$% (
)
)*+, -. / 0 . #
1 2 3 4 5 6
(
#$
7 89 :; 1:< =8 + > ?@A -
B *C D E -< . F& $#
72*; 2 G .
Hyalomma anatolicum anatolicum
9 Boophilus microplus
728 C*+ H
-I H J $ *
.
K L :' 0 M9 H 7 89 H : N+ '9 + > N;O9
)
4 P4 P
PP Q QP
KDa (
2 R7 H
H. anatolicum anatolicum -
7 S 7 89 : F& &: !K $TB78 F
B. microplus
-I H J $ * 1 ;& - 0 H9@ + .
.
S 7 89 ;8 < N. #
D E F < 7 H. anatolicum
anatolicum 9
B. microplus -A ?)<
U,
=V &
/
$&
,
=V &
/
- * W < $&
.
*R. 0 0 J&, @
:; *< FJ X. Y*7
7 89 D E # Z
2 - [ 7T* &
.
Protein bands of the cuticle of Hyalomma anatolicum anatolicum and Boophilus
microplus using electrophoresis technique
L.Y. Khalil
Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq
Abstract
In this study by using SDS-PAGE electrophoresis different bands of cuticular proteins of engorged female ticks,
Hyalomma anatolicum anatolicum and Boophilus microplus, was clarified six different bands of proteins with molecular
weights (182, 68, 67, 66, 57, 56 KDa) of H.anatolicum anatolicum while any band of proteins in Boophilus microplus was not
separated because it dissolved quickly in electrophoresis solution. The cuticular protein determinants of H.anatolicum
anatolicum and B. microplus were 4.7 mg/ml, 1.3 mg/ml, respectively. It may be concluded that protein bands of cuticle could be used as one of taxonomic parameters of ticks.
Available online at http://www.vetmedmosul.org/ijvs
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)
gel electrophoresis
;' 7 ;* S 72* =A 8
S 7 89 . : 89 1:< h V
)
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.(
) -(
2 F& ' -+ +
i H W < c * A + > ?@ H
G . -< . F H B 7T*
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anatolicum 9
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H.anatolicum anatolicum 9
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o
p
^6 $ < ^ 5 F< H 72*; m 2 G . N
=2 &9 [ b. 5 E& C*+ H =R q ;& 1 5 W < ! I N;89
=R -; E*
Stereoscopic dissection
microscope
e< F& S *; $# m B8 =89
ﺧ
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Sodium dodecyl
sulphate-polyacrylamide gel-electrophoresis (SDS-PAGE)
- d =8 S 7 < D E
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-I H J $ * H X 72*H u C v> 1 ;
. (Tris-HCl
buffer pH 6.8 containing 5% SDS. 30 mµ EDTA, 25%
glycerol and 2.5% mercabtoethanol).
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(
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U,Q % (
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R H9 =+
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4
.(
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: # *H
, %
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oxidase, Bovine serum albumin and α-amylase
( ) (
.
,
+ -.
!
x C*R& - 7 89 H : N+ '9 + > ?@A N7 H
D E
G . H "I _9 ^& d 0 H H !@ 9
7 H.anatolicum anatolicum H S 72*;
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L : . M9 -* 9
) 56,57,66,67,68,182 KDa (
* W <
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.
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:
x C*R& - 7 89 :;
D E . F& 2
H.anatolicum anatolicum H TB&
gI B% +
)
! #
%
0 M9 $/ 8 > R ' W < >
0 * J H L :
.
x C*R& S 7 89 H 1:< " T F& 0 # F - D E
G . B.microplus
H 72*;
72* ?@A + H
.
) -(
;8 =8 # : # 8
F 2 5 C*+ H F 89
-!>
x C*R& - D> ; D E
F < 7 )J U,
=V &
/
9 $&
,
=V &
/
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.
/ &
- 7 89 H : N+ '9 W + > ?@A YI *. S> 6
x C*R& D E
- O . 2
H.anatolicum anatolicum
L :' 0 M9 H9
) 56,57,66,67,68,128
0 * #
(
= F -
7 - S 7 89 ?@A $T 0 J& H FJ
.
microplus B
$ * 728 - & C*R $ ; - . H9@
-I H J
0 A r _ - " R 0 # H>9
S 7 89 -R I 0 J
2 5 D E 7 - B. microplus
D> < F< 9 I 7H S
L5 9 L :' 0 M9 S _ 7 & h F& B w& D V% '
I> $ ; - 0 H9@ +
) .(
0
T C*R& - S 7 89 $T
S D E H *
$/& . ?@A [ 7T8 - D # D I 2 F& B *C& . 7 Rhipicephalus appendiclatus 7 9
Ixodes ricinus
Q 9
- * W < 0 * #
) .(
x C*R S 7 89 L :' 0 M9 !K > ] < 0 #
D E 7 fH * ! B.microplus
?@A 0 H9_ W a:
2H + X D> 6 N 8 # I> $ ; - S 7 89
_
W < 0 J8 W R8 S 7 89 H F& B w& " d
ecdysone proteins
-+ + F 89 7&9
20-hydroxyecdysone
: !@ 9
2 5 1 TB. < f& 5 BTH X. J8
D E
F< 2
1)ﺧ D
2 l)R. <
W R8 H
Polysis
O 9
D ' S 7 89 H D E
) .(
F 8
0 0 e + > ?@A
-x C*R& D E
W $
A9 - I _ 5 !_9 ^& V
& 8 XH E&
XB%9
) (
X*+ > -
S T C*R 0 F&
D E 7
Ixodes holocyclus
9 H 72*; G . ;89
0 M9 H
L :'
P
& d 0 H N. # 0 * #
- I _9 ^
.
F& D *B - 2 R' 0 M9 iBC78
P W S < +
0 9
?@A =R 0 M9 - 5
D J $ - :
- 89>_ W $T* 2 G . - i y *. F&
[ e W < F& 5 2+ H fH
) (
S 728 C*+ H9
728 $/& B *C& ; R S ;R
L :
lecular
surface films
; ( 6 H =R 9
infra-red
spectroscopy .
2
- E 2 0 H y *7*+ =8
D EH 7
B.microplus F& 5 R R *+ F& -+ + $JEH B w&
c * =* = X. 1 ;J 9 h
2 S . J W <
2 ?@ 0 J a ﺧ
.
^ * + > ?@A YI *. F H ;O 9 (> S ] 9
. F& 2 H B.microplus
+ > YI *.9
)
U
(
7 2
I.ricinus F& S L :' W < ! ; !@ 9
D E
H9z_ d
*H $+)+ W rJB*8 & 7< q2 I _ W 1 ;*8 D V%
*+ D *& S J+9
acetyled polysaccharide )
Q
.(
" T 2 . F& . $# 0 H + > ?@A F& Y*7*R. S 7 89 H X D EH
S _ 0 J8 XH % ﺧ - D # D I
=R S 7 89 H $ ;8 W < D * B 7T* S + >
.
0 -&
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