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Name: Carla Cristina Mestre Veríssimo Dias Nationality: Portuguese Date of birth: December 15, 1989 Researcher ID: P-4060-2016 Current Address: Rua Guerra Junqueiro, nº7 6ºB ORCID: 0000-0002-3282-4210 2730-094 Barcarena
Phone: +351918058232 E-mail: carlamestredias@hotmail.com
Education
2013 Master in Biochemistry, Faculdade de Ciências e Tecnologias, Universidade de Coimbra (FCTUC), Portugal
2011 Degree in Biochemistry, Faculdade de Ciências e Tecnologias, Universidade de Coimbra (FCTUC), Portugal
Current and previous positions
Professional Experience
Oct 2015/June 2016 Study support teacher, Academia de Estudos “O Professor Voltinhas”, Lisboa (Portugal)
Previous and Current Scientific Activities
Sept 2013/June 2015 Researcher, BI - FCOMP-01- 0124-FEDER-019317, Laboratório Nacional de Energia e Geologia, FCT, Lisboa (Portugal)
Sept – Oct 2013 Entrepreneurship fellowship, IAPMEI – Agência para a Competitividade e Inovação, I. P., Lisboa (Portugal)
Jan – Aug 2013 Researcher - InovContacto Programme, Unidad de Regeneración Neural, Instituto de Salud Carlos III, Madrid, Spain
Sept 2011/Apr 2012 Intership, Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid (Spain)
Feb 2010/Jan 2011 Undergraduated researcher - BII, Laboratório de Electroanálise e Corrosão, Instituto Pedro Nunes, FCT, Coimbra (Portugal)
Feb/June 2010 Intership, Instituto de Microbiologia da Faculdade de Medicina, Coimbra (Portugal)
Feb 2009/Jan 2010
Undergraduated researcher - BII, Unidade de Química e Física Molecular no Departamento de Química, Coimbra (Portugal)
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Fellowships and awards
Sept 2013/June 2015 Fellowship for master researchers (BI), Laboratório Nacional de Energia e Geologia, FCOMP-01- 0124-FEDER-019317, FCT, Lisboa (Portugal) Sept – Oct 2013 Entrepreneurship fellowship, IAPMEI – Agência para a Competitividade
e Inovação, I. P., Lisboa (Portugal)
Jan – Aug 2013 InovContacto fellowship, Unidad de Regeneración Neural, Instituto de Salud Carlos III, Madrid, Spain
2012/2013 Award for the 3% best students of the year in the Biochemistry Master Feb 2010/Jan 2011 Fellowship for undergraduated researchers (BII), L-Dopa and
Epinephrine electrochemical study, Laboratório de Electroanálise e Corrosão, Instituto Pedro Nunes, FCT, Coimbra (Portugal)
Feb 2009/Jan 2010 Fellowship for undergraduated researchers (BII), Study of the role that the alterations of the energetic metabolism noticed in most of cancer cells (fermentative flux increase and cellular respiration decrease – Warburg effect) can have in the promotion and development of cancer, Unidade de Química e Física Molecular no Departamento de Química, FCT, Coimbra (Portugal)
Publications
Scientific papers in peer-review international journals and citation
1. Ana Paula Batista, Emílio Palomo López, Carla Dias, Teresa Lopes da Silva, Isabel Paula Marques (2017) “Wastes valorization from Rhodosporidium toruloides NCYC 921 production and biorefinery by anaerobic digestion”, Bioresource Technology, 226: 108-117 doi: 10.1016/j.biortech.2016.11.113
(NC=4, IF=4,917)
2. Dias C, Silva C, Freitas C, Reis A, da Silva TL (2016) “Effect of Medium pH on
Rhodosporidium toruloides NCYC 921 Carotenoid and Lipid Production Evaluated by Flow
Cytometry”, Appl Biochem Biotechnol, 179: 776-87 doi: 10.1007/s12010-016-2030-y
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3. Dias C, Sousa S, Caldeira J, Reis A, Lopes da Silva T (2015) “New dual-stage pH control fed-batch cultivation strategy for the improvement of lipids carotenoids production by the red yeast Rhodosporidium toruloides NCYC 921”, Bioresource Technology, 189: 309-18 doi:
10.1016/j.biortech.2015.04.009
(NC=4, IF=4,917)
4. Herrero de Dios C, Román E, Diez C, Alonso-Monge R, Pla J (2012) “The transmembrane protein Opy2 mediates activation of the Cek1 MAP kinase in Candida
albicans”, Fungal Genet Biol 50: 21-32 doi: 10.1016/j.fgb.2012.11.001
(NC=10, IF=2,993)
Communications
Oral Communications by invitation
1. Carla Dias. Estudo Electroquímico de L-Dopa e Epinefrina. 1º Simpósio CEMUC, Departamento de Engenharia Mecânica da Universidade de Coimbra, Portugal, 7 July 2011.
Other Oral Communications
1. Alberto Reis, Carla Dias, João Caldeira, Marta Teles, Bruno Oliveira, Paula Cristina Passarinho, Teresa Lopes da Silva. Downstream processing towards a yeast biorefinery: biodiesel and fine carotenoids from Rhodosporidium toruloides NCYC 921. 12th Yeast Lipid Conference, Ghent, Belgium, 20-22 May 2015.
2. Carla Dias, Sofia Sousa, Cláudia Freitas, João Caldeira, Beatriz Nobre, Alberto Reis, Teresa Lopes da Silva. Third generation biofuels and high value added products from the yeast
Rhodosporidium toruloides NCYC 921: A possible approach for reducing the current fossil oil
dependence. XX Jornadas de Leveduras Professor Nicolau Van Uden, Instituto Superior Técnico, Universidade de Lisboa, Portugal, 11-12 July 2014.
3. Carla Dias, Corália Silva, Cláudia Freitas, Alberto Reis, Teresa Lopes da Silva.. Physiological stress response of the yeast Rhodosporidium toruloides NCYC 921 to pH changes grown on batch cultures for lipids (biodiesel) and carotenoids production. XX Jornadas de Leveduras Professor Nicolau Van Uden, Instituto Superior Técnico, Universidade de Lisboa, Portugal, 11-12 July 2014.
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Poster Communications
1. Alberto Reis, Carla Dias, João Caldeira, Marta Teles, Bruno Oliveira, Paula Cristina Passarinho, Teresa Lopes da Silva. Downstream processing towards a yeast biorefinery: biodiesel and fine carotenoids from Rhodosporidium toruloides NCYC 921. 12th Yeast Lipid Conference, Ghent, Belgium, 20-22 May 2015.
2. Carla Dias, Sofia Sousa, Cláudia Freitas, João Caldeira, Beatriz Nobre, Alberto Reis, Teresa Lopes da Silva. Third generation biofuels and high value added products from the yeast
Rhodosporidium toruloides NCYC 921: A possible approach for reducing the current fossil oil
dependence. XX Jornadas de Leveduras Professor Nicolau Van Uden, Instituto Superior Técnico, Universidade de Lisboa, Portugal, 11-12 July 2014.
3. Carla Dias, Corália Silva, Cláudia Freitas, Alberto Reis, Teresa Lopes da Silva. Physiological stress response of the yeast Rhodosporidium toruloides NCYC 921 to pH changes grown on batch cultures for lipids (biodiesel) and carotenoids production. XX Jornadas de Leveduras Professor Nicolau Van Uden, Instituto Superior Técnico, Universidade de Lisboa, Portugal, 11-12 July 2014.
4. Carla Dias. Estudo Electroquímico de L-Dopa e Epinefrina. 1º Simpósio CEMUC, Departamento de Engenharia Mecânica da Universidade de Coimbra, Portugal, 7 July 2011.
5. Ana Henriques, Carla Dias, Diana Carvalho, Sílvia Andrade. Herpes Simplex e Herpes Zoster. III Encontros de Infecção e Imunidade, Museu da Ciência da Universidade de Coimbra, 12-13 May 2009.
Erasmus
Sept 2011/Apr 2012 Master Thesis “Candida albicans OPY2 gene complementation in
Saccharomyces cerevisiae”, Departamento de Microbiología II,
Facultad de Farmacia, Universidad Complutense de Madrid, Madrid (Spain)
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Thesis
2012 Master thesis “Candida albicans OPY2 gene complementation in Saccharomyces
cerevisiae” – final grade: 18 out of 20.
2010 Graduation thesis “Identification and enumeration of pathogenic yeast by molecular biology techniques” – final grade: 18 out of 20.
Scientific Projects
Team Member
2013/2015 CAROFUEL - New process for a sustainable microbial biodiesel production: The yeast Rhodotorula glutinis biorefinary as a source of biodiesel, biogas and carotenoids, Laboratório Nacional de Energia e Geologia, FCOMP-01- 0124-FEDER-019317, FCT, Lisboa (Portugal)
2010/2011 L-Dopa and Epinephrine electrochemical study, Laboratório de Electroanálise e Corrosão, Instituto Pedro Nunes, FCT, Coimbra (Portugal)
2009/2010 Study of the role that the alterations of the energetic metabolism noticed in most of cancer cells (fermentative flux increase and cellular respiration decrease – Warburg effect) can have in the promotion and development of cancer, Unidade de Química e Física Molecular no Departamento de Química, FCT, Coimbra (Portugal)
Professional and Personal Competences
Expertise in Microbiology working with several yeast and E.coli.
Excellent skills in molecular biology techniques using DNA, RNA and Proteins such as DNA extraction, PCR, gel electrophoresis, plasmid isolation, protein extraction, Western Blotting, among others.
Experience in the work with 5L and 35L bioreactors in batch and fed-batch.
Experience in lipids extraction, fatty acids transmetilation, carotenoids extraction, nitrogen and glucose quantification.
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Expertise in flow cytometry.
Experience in cell culture with different cell lines and cell biology methodologies.
Skills in fluorescence microscopy methodologies, immunohistochemistry, immunocytochemistry, GC, HPLC, UV-spectroscopy.
Portuguese (Native), English (CAE), Spanish (Independent Level).
Good skills in Microsoft Word, Microsoft PowerPoint, Microsoft Excel, GraphPad, Bioinformatics and Scientific Databases (NCBI, SGD, BRENDA, PDB, Blast, ClustalW).
Good communications skills and ability to work independently, organization, responsibility, team spirit and ability to work as a team member, creativity, enthusiastic, calm and patient.