deletion mutant
CONSTRUCTION AND CHARACTERIZATION OF A GLYCOPROTEIN E DELETION MUTANT OF BOVINE HERPESVIRUS TYPE 1.2 STRAIN ISOLATED IN BRAZIL
... a deletion of the glycoprotein E (gE) gene. The deletion was introduced by co- transfection of a deletion fragment containing the 5´and 3´gE flanking regions and genomic DNA of wild type BoHV-1 into ...
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Antibodies with higher bactericidal activity induced by a Neisseria gonorrhoeae Rmp deletion mutant strain.
... the mutant and wild-type strains were capable of inducing high-titer antibodies in mice since mCEACAM1 cannot bind ...Rmp deletion mutant strain constructed herein is feasible for developing novel ...
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Modulation of ocular surface glycocalyx barrier function by a galectin-3 N-terminal deletion mutant and membrane-anchored synthetic glycopolymers.
... Figure S2 Cloning strategy for the generation of full- length galectin-3 and a galectin-3 N-terminal deletion mutant. (A) Galectin-3 mRNA extracted from HCLE cells was reverse transcribed, amplified by PCR, ...
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CONSTRUCTION AND CHARACTERIZATION OF A BOVINE HERPESVIRUS 5 MUTANT WITH A DELETION OF THE GI, GE AND US9 GENES
... gI/gE/US9 deletion mutant of BoHV- 5, plasmid pAC226 was linearized with HindIII, 722 nucleotides upstream to the insert, site localized in the vector, and co- transfected with genomic DNA of wild type EVI ...
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Phosphatase complex Pph3/Psy2 is involved in regulation of efficient non-homologous end-joining pathway in the yeast Saccharomyces cerevisiae.
... cerevisiae. Deletion of either PPH3 or PSY2 genes reduced NHEJ efficiency both in the context of chromosomal and plasmid DSB ...Double deletion mutant strains for either PPH3 or PSY2 with CHK1 showed ...
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Cross-Regulation between the phz1 and phz2 Operons Maintain a Balanced Level of Phenazine Biosynthesis in Pseudomonas aeruginosa PAO1.
... mechanism which mediates the expression of the two phz operons. To test this hypothesis, we further constructed the translational fusion mutants on the chromosome with the truncated lacZ in frame. The assessment of ...
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Biofilm forming ability and exoproteomic analysis of Listeria monocytogenes
... A deletion mutant strain on this gene was produced (3119 lmo2504 ) and its biofilm forming ability compared to the wild type using the crystal violet and the ruthenium red assays, as well as scanning ...
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Dam methylation participates in the regulation of PmrA/PmrB and RcsC/RcsD/RcsB two component regulatory systems in Salmonella enterica serovar Enteritidis.
... dam mutant does not restore the defective LPS ...dam mutant, PmrA levels remained ...rcsB mutant, indicating that RcsB induces wzz gene ...pmrA deletion mutant the amount of wzz mRNA ...
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The PduQ enzyme is an alcohol dehydrogenase used to recycle NAD+ internally within the Pdu microcompartment of Salmonella enterica.
... pduQ deletion, but not due to polarity or an unknown ...pduQ deletion mutant even though its enzymatic activity was similar ...DpduQ deletion mutant when induced with IPTG at concentra- ...
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Characterization of Novel Factors Involved in Swimming and Swarming Motility in Salmonella enterica Serovar Typhimurium.
... ydiV deletion resulted in increased motility due to upregulation of FlhDC ...the deletion mutant on swarming agar ...ΔydiV mutant indicating that YdiV is not involved in repression of type I ...
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Involvement of a velvet protein FgVeA in the regulation of asexual development, lipid and secondary metabolisms and virulence in Fusarium graminearum.
... the mutant (Table ...the deletion of a type 2C protein phosphatase gene FgPTC3 also led to accumulation of large lipid droplets in ...FgVEA deletion mutant, including reduction in aerial ...
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S. pombe kinesins-8 promote both nucleation and catastrophe of microtubules.
... klp5/6 deletion mutant, in which the absence of Klp5 or Klp6 reduces nucleation activity, weakens cross-linking in the IMAs and increases the dwell time of the microtubule tips at the cell end before they ...
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PfsR is a key regulator of iron homeostasis in Synechocystis PCC 6803.
... pfsR deletion mutant under the few growth conditions (including low light, high light, and iron limitation) we tested in the laboratory, however, we cannot rule out the possibility that the mutant ...
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A LacI-family regulator activates maltodextrin metabolism of Enterococcus faecium.
... ΔmdxR mutant was first compared to the transcriptome of its parental strain ...ΔmdxR mutant strain in comparison to the parental strain during growth in M1 with ...ΔmdxR mutant, which confirmed the ...
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Rac1 is required for pathogenicity and Chm1-dependent conidiogenesis in rice fungal pathogen Magnaporthe grisea.
... Mgrac1 deletion mutants cannot effectively infect rice leaves and roots, leading to loss of ...Mgrac1 deletion mutants, the dominant negative transformant is also defective in the formation of conidia and ...
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Syntaxin 1B, but not syntaxin 1A, is necessary for the regulation of synaptic vesicle exocytosis and of the readily releasable pool at central synapses.
... To investigate the common neural function of STX1, we produced STX1A/1B double knockout (DKO) mice. Because the DKO mice were embryonic lethal, cortical cultures were prepared from embryonic day (E) 12.5 fetuses. ...
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No evidence for cardiac dysfunction in Kif6 mutant mice.
... KIF6 mutant and wild type littermates, and conducted RT-PCR with forward and reverse primers located Figure ...cells, mutant mice and structural ...and mutant mice. (E) cDNA sequence of the ...
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Rare human diseases: 9p deletion syndrome
... Abstract. Rare human diseases: 9p deletion syndrome. Galagan V.O. Objective of the study was to review the anamnesis, pheno - and genotype in patients with rare chromosome disorders such as 9p deletion ...
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Secure Deletion of Data from SSD
... Abstract—The deletion of data from storage is an important component on data ...The deletion of entire disc or special files is well-known on hard drives, but this is quite different on SSDs, because they ...
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Protease activity of PprI facilitates DNA damage response: Mn2+-dependence and substrate sequence-specificity of the proteolytic reaction.
... In bacteria such as Escherichia coli, the SOS response is activated after exposure to ultravio- let, ionizing radiation or chemical mutagens [24–26]. However, the classic SOS response sys- tem does not exist in ...
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