high throughput screening
A High Throughput Screening Assay for Anti-Mycobacterial Small Molecules Based on Adenylate Kinase Release as a Reporter of Cell Lysis.
... Mycobacterium tuberculosis (Mtb) is well-established to be one of the most important bac- terial pathogens for which new antimicrobial therapies are needed. Herein, we describe the development of a high ...
14
Use of activity-based probes to develop high throughput screening assays that can be performed in complex cell extracts.
... One of the most common techniques used by the pharmaceu- tical industry to identify novel drug leads is high throughput screening (HTS). This method allows inhibition effects of large numbers of ...
6
A multi-parameter, high-content, high-throughput screening platform to identify natural compounds that modulate insulin and Pdx1 expression.
... novel high-throughput screening platform that exploits multi- parameter high-content analysis to determine the effect of compounds on beta-cell survival, as well as the promoter activity of ...
9
A Sensitive and Robust High-Throughput Screening Assay for Inhibitors of the Chikungunya Virus nsP1 Capping Enzyme.
... nsP1 is a 535 amino acid protein that is proteolytically cleaved from the viral polyprotein during positive strand synthesis to yield the active protein form [19]. nsP1 contains a mem- brane anchor helix that attaches ...
14
High-throughput screening reveals alsterpaullone, 2-cyanoethyl as a potent p27Kip1 transcriptional inhibitor.
... p27 Kip1 is a cell cycle inhibitor that prevents cyclin dependent kinase (CDK)/cyclin complexes from phosphorylating their targets. p27 Kip1 is a known tumor suppressor, as the germline loss of p27 Kip1 results in ...
10
Integrating virtual and high-throughput screening: opportunities and challenges in drug research and development.
... - high throughput screening) e de triagem virtual (VS - virtual scre- ening) têm ocupado papel de destaque entre as estratégias modernas exploradas na identificação de novas substâncias ...
9
High-throughput screening assay used in pharmacognosy: Selection, optimization and validation of methods of enzymatic inhibition by UV-visible spectrophotometry
... In research laboratories of both organic synthesis and extraction of natural products, every day a lot of products that can potentially introduce some biological activity are obtained. Therefore it is necessary to have ...
13
High throughput screening for anti-Trypanosoma cruzi drug discovery.
... for high throughput screening (HTS) as well as the establishment of new in vivo protocols that allow faster experimental ...automated high content microscopy approach- es have also been used ...
6
An assay suitable for high throughput screening of anti-influenza drugs.
... The most commonly used targets for antiviral drugs are M2 ion channel or neuraminidase encoded by the viral genome [4,5]. M2 protein, which is crucial for viral infection, forms a tetrameric proton channel in the host ...
5
High-content, high-throughput screening for the identification of cytotoxic compounds based on cell morphology and cell proliferation markers.
... compound screening. The use of high-content imaging in primary screening is becoming more widespread, and by utilising phenotypic approaches it should be possible to incorporate cytotoxicity ...
8
TRAIL-Based High Throughput Screening Reveals a Link between TRAIL-Mediated Apoptosis and Glutathione Reductase, a Key Component of Oxidative Stress Response.
... A high throughput screen for compounds that induce TRAIL-mediated apoptosis identified ML100 as an active chemical probe, which potentiated TRAIL activity in prostate carcinoma PPC-1 and melanoma MDA-MB-435 ...
26
Tackling heterogeneity: a leaf disc-based assay for the high-throughput screening of transient gene expression in tobacco.
... cut according to the schema shown in Figure 1A to assess the impacts of leaf age and position within the leaf on the rpa levels. Following extraction, the TSP was determined using the Bradford assay, and the recombinant ...
8
A screen against Leishmania intracellular amastigotes: comparison to a promastigote screen and identification of a host cell-specific hit.
... cell-based screening assay using a human macrophage cell line infected with ...enables screening of compounds directly against the intracellular stage of the ...this screening method with traditional ...
9
Clinics vol.62 número6
... richness. Screening programs have been established in Brazil as a strategy to identify potentially active ...substances. High throughput screening techniques allow for the analysis of large ...
6
Nanoscale Synaptic Membrane Mimetic Allows Unbiased High Throughput Screen That Targets Binding Sites for Alzheimer's-Associated Aβ Oligomers.
... to high-throughput screening (HTS) tools designed for soluble ...with high affinity and specificity, with binding dependent on intact synaptic membrane proteins, and selective for the higher ...
24
Genome-scale analysis of Mycoplasma agalactiae loci involved in interaction with host cells.
... to study the minimal set of essential genes [14–16], but has also been successfully employed in a few cases to identify genes potentially involved in pathogenicity [17], gliding motility and adherence [18,19]. Such an ...
11
Screening for novel LRRK2 inhibitors using a high-throughput TR-FRET cellular assay for LRRK2 Ser935 phosphorylation.
... a high-throughput screening (HTS) compatible Time- Resolved Fo¨rster Resonance Energy Transfer (TR-FRET) assay using either LRRKtide or Nictide as the substrate [5,23,24], standard radioactive ...
12
Síntese e avaliação de bioatividades de derivados do ácido cinâmico contendo o núcleo 1,2,3-triazólico
... Antes do surgimento do high-throughput screening cerca de 80% dos fármacos eram produtos naturais ou inspirados em um composto de origem natural (CALDERON et al., 2009). O ácido [r] ...
279
Analysis of high-throughput sequencing and annotation strategies for phage genomes.
... ARACHNE with all the default settings except ‘recycle_bad_con- tigs’ which was set to ‘false’ to ensure the assembler did not exclude small, low-coverage contigs. We assembled data generated by 454 using the Newbler ...
12
NSC23925, identified in a high-throughput cell-based screen, reverses multidrug resistance.
... Methodology/Principal Findings: To identify small molecule compounds that can reverse chemoresistance, we developed a 96-well plate high-throughput cell-based screening assay in a paclitaxel ...
12