Metallo-β-lactamase
ISOLATION OF THE FIRST IMP-4 METALLO-Β-LACTAMASE PRODUCING KLEBSIELLA PNEUMONIAE
... This study shows for the first time the mechanism of carbapenem resistance of a Klebsiella pneumoniae clinical isolate TJ8 recovered from Tianjin Medical University General Hospital ,China. The modified Hodge test and ...
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Purification of Metallo-β-Lactamase CcrA from Bacteroides
... the metallo-β-lactamase CcrA from Bacteroides fragilis based on the plasmid pMSZ02, in which the crude protein secreted into growth medium was precipitated by 80% sulfate saturation of the medium and ...
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Mechanisms of carbapenem resistance in endemic Pseudomonas aeruginosa isolates after an SPM-1 metallo-β-lactamase producing strain subsided in an intensive care unit of a teaching hospital in Brazil
... Carbapenem-resistance mechanisms are a challenge in the treatment of Pseudomonas aeruginosa infections. We investigated changes in P. aeruginosa carbapenem-resistance determinants over a time period of eight years after ...
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Review Article Prevalence of metallo-β-lactamase-encoding genes among carbapenem-resistant Pseudomonas aeruginosa strains isolated from burn patients in Iran
... of metallo-β-lactamases [(MBLs), including Verona Integron-encoded Metallo-beta-lactamase (VIM), imipenemase (IMP), São Paulo metalo-beta-lactamase (SPM), German imipenemase (GIM), New ...
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Major Article Phenotypic and molecular detection of metallo-β-lactamase-producing Pseudomonas aeruginosa isolates from patients with burns in Tehran, Iran
... of β-lactams, including penicillins, cephalosporins, and carbapenems 7 ...integron-encoded metallo-β-lactamase (VIM), São Paulo metalo-β-lactamase (SPM), Germany imipenemase ...
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New Delhi metallo-β-lactamase (NDM-1): how real is the threat?
... During the past decade the escalation of antimicrobial resistance in Enterobacteriaceae has become a major concern worldwide. Antimicrobial resistance among Enterobacteriaceae is growing, largely due to broad spectrum ...
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Identification of New Delhi metallo-β-lactamase 1 in Acinetobacter lwoffii of food animal origin.
... spectrum beta-lactamase gene. Antimicrob Agents Chemother 50: 1745–1752. 24. Adams MD, Chan ER, Molyneaux ND, Bonomo RA (2010) Genome wide analysis of divergence of antibiotic resistance determinants in closely ...
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A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase.
... These PCR products were digested with BamHI and XhoI and ligated into pET28a (Novagen, Inc., Madison, WI). The plasmids were used to transform E. coli BL21-CodonPlus (DE3)-RIP (Agilent Technologies, Santa Clara, CA), and ...
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Metallo-β-lactamase-production in meropenem-susceptible Pseudomonas aeruginosa isolates: risk for silent spread
... The aim of this study was to characterize two metallo-β-lactamases (MBLs)-producing Pseudomonas aeruginosa clinical isolates showing meropenem susceptibility. Antimicrobial susceptibility was assessed by ...
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J. Bras. Patol. Med. Lab. vol.51 número5
... The adequate identiication of strains with MβL-encoding genes is important to guide therapeutic choices in public and private hospitals and can give support to better treatments against these infections. Thus, control ...
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Uma revisão sobre metalo-β-lactamases.
... of metallo-b-lactamase gene bla IMP-4 among gram-negative pathogens in a clinical setting in ...of metallo-β-lactamase- producing Klebsiella pneumoniae isolated from blood ...producing ...
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Cooperative and harmful behaviour in the bacterial world
... of β- lactamases coded in the plasmids of natural origin, RP4, R16a or ...a β-lactamase enzyme that is sufficient to locally detoxify the environment and save the producer from the effect of ...
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Biochemical characterization of CTX-M-15 from Enterobacter cloacae and designing a novel non-β-lactam-β-lactamase inhibitor.
... harbouring bacterial population, as the consumption of these antibiotics in the treatment of bacterial infections is low. The MIC results were well supported by steady-state kinetics data, which revealed that CTX-M-15 ...
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Caracterização dos elementos genéticos moveis responsáveis pela disseminação de genes associados a resistência bacteriana em Pseudomonas spp. isoladas na América Latina
... Characterization of Class 1 integrons from Pseudomonas aeruginosa that contain the blaVIM-2 carbapenem-hydrolyzing β -lactamase gene and of two novel aminoglycoside resistance gene[r] ...
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Clonal relation and antimicrobial resistance pattern of extended-spectrum β-lactamase- and AmpC β-lactamase- producing Enterobacter spp. isolated from different clinical samples in Tehran, Iran
... of hydrolyzing penicillins, broad-spectrum cephalosporins, and aztreonam, but may not hydrolyze cephamycin, and are inhibited by clavulanic acid. AmpC β-lactamases are usually encoded on the bacterial chromosome ...
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A β-Lactamase based reporter system for ESX dependent protein translocation in mycobacteria.
... Figure 3. Protein expression of BlaTEM reporter constructs shown by Western blot analysis. Fusion-proteins expressed from the nine reporter vectors (pOLYG-blaTEM (0) and pI-blaTEM to pVIII-blaTEM) were separated using ...
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Ocorrência de cepas de Pseudomonas aeruginosa multirresistentes produtoras de metalo-β-lactamase blaSPM-1 em amostras clínicas.
... of metallo- Sader HS, Reis AO, Silbert S, Gales ...of metallo- IMPs, VIMs and SPMs: the diversity of metallo- b-lactamases produced by carbapenem-resistant Pseudomonas aeruginosa in a Brazilian ...
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Extended-Spectrum β-Lactamases and/or Carbapenemases-Producing Enterobacteriaceae Isolated from Retail Chicken Meat in Zagazig, Egypt.
... of β-lactamase-encoding genes DNAs of all phenotypic ESBL- and carbapenemases positive isolates were extracted by boiling lysis method as described previously ...
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CHARACTERIZATION OF EXTENDED-SPECTRUM Β-LACTAMASE-PRODUCING ESCHERICHIA COLI STRAINS ISOLATED FROM DAIRY PRODUCTS
... BARTHELEMY, M., PE.DUZZI, J., BERNARD, H., TANCRE'DE, C., LABIA, R. 1992. Close amino-acid sequence relationship between the new plasmid- mediated extended-spectrum L-lactamase MEN-1 and chromosomally encoded ...
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EXTENDED-SPECTRUM βββββ-LACTAMASES IN KLEBSIELLA SPP AND ESCHERICHIA COLI OBTAINED IN A BRAZILIAN TEACHING HOSPITAL: DETECTION, PREVALENCE AND MOLECULAR TYPING Ana Lúcia Peixoto de Freitas 2,3 ; Denise Pires Machado1 ; Fabiana da Silva Correa Soares1 ; Af
... the β-lactamase enzyme by clavulanic acid (double- disk method and MIC CAZ/CAC method) one will observe very similar results: 19 positive isolates in DD method and 18 isolates in MIC CAZ/CAC (Table ...
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