Paraffin-embedded tissue
Whole-genome gene expression profiling of formalin-fixed, paraffin-embedded tissue samples.
... To assess the reproducibility of the WG-DASL assay we initially used inputs of 200 ng total RNA extracted from six different ovarian tumor FFPE tissues. The samples were labeled and hybridized as described in the ...
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KLC1-ALK: a novel fusion in lung cancer identified using a formalin-fixed paraffin-embedded tissue only.
... Anti-ALK immunohistochemistry may thus be useful to detect candidate tumors for a novel ALK fusion. However, to identify the fusion partner, other molecular techniques are usually required such as 59-rapid amplification ...
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A Method to Correlate mRNA Expression Datasets Obtained from Fresh Frozen and Formalin-Fixed, Paraffin-Embedded Tissue Samples: A Matter of Thresholds.
... Whole-genome microarray gene expression profiling has become an important tool for the discovery of prognostic and predictive genes for various human cancers [1,2]. Until recently, the main and preferred source of gene ...
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High fidelity copy number analysis of formalin-fixed and paraffin-embedded tissues using Affymetrix Cytoscan HD chip.
... The current methodologies to detect chromosome abnormalities include Karyotyping using Giemsa staining of chromosomes, high throughput array analysis of single nucleotide polymorphism and DNA copy number, and whole ...
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Histological analysis of SLC38A6 (SNAT6) expression in mouse brain shows selective expression in excitatory neurons with high expression in the synapses.
... Previously characterized SLC38 proteins have been found to localize either in the neurons or in astrocytes [10–12] or in both type of cells [9,32,33]. Our co-localization studies using immuno- histological double ...
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Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction
... DNA extraction - For each sample five to seven 5 µm sections of paraffin-embedded tissue were placed in a tube. The samples were washed with xilene, incubated for 30 min at 37ºC, centrifuged at ...
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Trypanosoma cruzi: parasite persistence in tissues in chronic chagasic Brazilian patients
... in paraffin-embedded tissue samples from chronic patients using NPCR (nested polymerase chain reaction) and QPCR (quantitative polymerase chain reaction) ...formalin-fixed ...
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Int. braz j urol. vol.40 número4
... Our study has several caveats that may compromise the generalization of its findings. First, it is a retrospective study, with the implied limitations in data collection. Second, the im- munohistochemical staining was ...
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STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.
... information. Paraffin-embedded tissues alleg- edly exhibit pronounced autofluorescence [23] and, although the attainable structural preservation of paraffin-embedded tissue is ...
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DETECTION OF PARVOVIRUS B19 INFECTION IN FORMALIN-FIXED AND PARAFFIN-EMBEDDED PLACENTA AND FETAL TISSUES
... in paraffin-embedded tissue: proper fixative, proper duration of fixation, proper age of the paraffin block, and proper length of the DNA fragment to be ...of paraffin and proteins that ...
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Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin- embedded healthy skin for the diagnosis of canine visceral leishmaniasis
... QUEIROZ, N.M.G.P.; ASSIS, J.; OLIVEIRA, T.M.F. et al. Diagnóstico da leishmaniose visceral canina pelas técnicas de imunoistoquímica e PCR em tecidos cutâneos sem associação com a RIFI e ELISA-teste. Rev. Bras. ...
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Arq Bras Endocrinol Metab vol.57 número6
... M uch interest has been focused in nucleic acid isolation from tissue samples sto- red for long periods and their utilization on molecular biomarkers research. However, sample ixation induces chemical modiications ...
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Is high resolution melting analysis (HRMA) accurate for detection of human disease-associated mutations? A meta analysis.
... Syto9), sample source (blood cell/bone marrow, fresh frozen (FF) tissue, formalin-fixed and paraffin-embedded (FFPE) tissue or cytologic slides), eligible/non-eligible sample size (eligible ...
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A pilot study on the identi fication of human papillomavirus genotypes in tongue cancer samples from a single institution in Ecuador
... HPV-associated HNC) rates given the prevalence of HPV 33, 45, 52, and 58 in this cohort, however, it is cost-pro- hibitive for the vast majority of the Ecuadorian population. This study had limitations, including its ...
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FORMULATION AND EVALUATION OF <i>FICUS BENGHALENSIS</i> AND <i>FICUS RACEMOSA</i> AQUOUES EXTRACTS
... In this study, ointment (A and B) was formulated with different bases like white soft paraffin, cetostearyl alcohol hard paraffin, light liquid paraffin and microcrystalline wax. From the study, it ...
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Epstein-Barr Virus (EBV) detection and typing by PCR: a contribution to diagnostic screening of EBV-positive Burkitt's lymphoma
... The sample included 38 Burkitt's lymphomas/L3-ALL (BL), 2 Burkitt's-like lymphomas (BLL) and 4 diffuse large B-cell lymphomas (DLBCL). Histopathological diagnosis was revised according to the R.E.A.L classification [15]. ...
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Detecção da fusão SS18-SSX em material parafinado e comparação de métodos moleculares...
... Detection of SS18-SSX fusion transcripts in formalin-fixed paraffin- embedded neoplasms: analysis of conventional RT-PCR, qRT-PCR and dual color FISH as diagnostic tools for synovial sa[r] ...
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Immunohistochemical detection of Clostridia species in paraffin-embedded tissues of experimentally inoculated guinea pigs
... formalin-fixed, paraffin-embedded tissues of guinea pigs, and it may complement the bacteriological procedures for the diagnosis of blackleg and malignant oedema, especially where laboratory facilities for ...
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Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples
... Objective: Adequate isolation of nucleic acids from peripheral blood, ine-needle aspiration cells in stained slides, and fresh and formalin-ixed/parafin-embedded tissues is crucial to en- sure the success of ...
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Using a Cluster for Securing Embedded Systems
... Gnort is a solution that takes advantage of the use of GPU but cannot achieve the performance of the use of a HPC cluster as Gnort uses a single NVIDIA GeForce 8 Series. It’s also a solution for GPU clusters and not for ...
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