[PDF] Top 20 Apoptotic mechanisms in rabbits with blast-induced acute lung injury

... Apoptosis in the lung tissue was detected by the TUNEL method according to the kit instructions; sections were de-waxed in xylene, followed by rehydration in descending concentrations of ... See full document

... role in the regulation of alternative splicing of nuclear factor-kB (NF-kB) in the lungs after ...TLR4 in the regulation of immunity has been well defined in previous studies; for example, ... See full document

... to lung repair mechanisms after ALI ...effect in vivo ...cells with two Yamanaka factors. These characteristics, together with absence of ethical issues concerning their employment, ... See full document

... pended in 1 ml of cold sterile PBS and total number of cells was immediately determined using ...min in a cytocentrifuge (Shandon Cytospin 2, Pittsburgh, ...stained with Diff-Quik Stain and ... See full document

... molecules in the blood and in the BAL were reduced or abrogated by the use of both AMβ81 and ...expression in the blood 6 hours after ALI (KW = ...9B) in the blood 6 hours after ... See full document

... 2007). In fact, several antigens derived from intracellular para- sites can be recognized by innate immune receptors on macrophages, triggering NOS2 activity (Xie et ... See full document

... right lung lobes (1×1×1mm) were also randomly sampled, followed by fixation in ...twice with 0.1M PBS (10min/rinse), they were post-fixed with 1% osmic acid for 2h, treated with acetone ... See full document

... LPS-induced lung injury in the rat is frequently used as a model for studying the ALI ...PMNs in the lung tissues, increased endothelial permeability and tissue damage, and ... See full document

... minutes, with an administration of a standard volume of saline solution (SS) 5 min before the end of the ischemia period; the clip was then releases or a 120-min period of reperfusion; (3) I+PTX+R: ischemia as ... See full document

... elevated in many inflammatory diseases of the respiratory ...involved in antimicrobial, anti-inflammatory and antioxidant effects, overproduction of NO may be harmful in conditions such as sepsis and ... See full document

... patients with essential hypertension or a high cardiovascular risk as well for congestive heart failure, diabetes nephropathy, or CKD, since they have been shown to either reduce mortality or to retard disease ... See full document

... dissolved in sterile PBS was introduced ...flat in a researcher’s ...study in which a single, large volume of good quality blood must be collected from experimental animals ...degermed with ... See full document

... injured lung is characterized by an excessive amount of extravascular lung water that accumulates in interstitial and alveolar compartments and by an infiltration of lung structures by ... See full document

... associated with fibrosis as examination of ALI/ARDS lungs and non-infected control lungs stained with Masson’s Trichrome (Figure 7A and D) showed Grade 0 fi- brosis (no ...areas with collagen (Figure ... See full document

... left lung harvested from each rat was infused with 5 mL ice-cold PBS, which was withdrawn and reinfused two more ...differences in the volume of bronchoalveolar lavage fluid (BALF) recovered ...the ... See full document

... mouse lung to radiation-induced pneumonitis and fibrosis is strain-dependent ...compared lung injury in two C3H, three C57BL and one CBA mouse strain (dose range studied was ...fibrosis ... See full document

... adicionado o polietilenoglicol (5%, massa/volume), não ocorreu uma redução da inibição do surfactante exógeno pelas proteínas presentes no edema alveolar na SDRA. Analisando-se tanto os valores de mecânica respiratória ... See full document

... changes in driving pressure. If the increase in the PEEP level leads to increased aeration of the lung tissue through recruitment, a decrease in driving pressure is ...recruit lung ... See full document

... Left lung tissue samples were fixed in 10% buffered formalin, dehydrated, cleared, and embedded in paraffin according to a routine ...stained with hematoxylin and eosin for light microscopic ... See full document

... explained in three ...patients with diseased kidneys and another for patients with healthy kidneys–may be needed for consistency among ...increase in sCr [44] or sCys C [45] above baseline was ... See full document