[PDF] Top 20 Generation of rat-induced pluripotent stem cells from a new model of metabolic syndrome.

... 293FT cells (Invitrogen; Carlsbad CA; ...6 cells per 100-mm dish in basic DMEM and incubated overnight. 293FT cells stably express the neomycin resistance gene and should be maintained in medium ... See full document

... T cells would provide new approaches to treatment of these devastating ...capable of differentiating into multiple blood cell ...stromal cells to induce hematopoietic cell lineage ... See full document

... demyelination of axons after one ...recruited from the adjacent tissues ...no cells and no vehicle. Cells were transplanted into the chiasm using a 25 m l Hamilton syringe connected to a 30 ... See full document

... source of cells for ...original cells reprogrammed; this precludes the need for complex procedures to create the genetic defect and avoids potential secondary effects of the genetic ... See full document

... Induced pluripotent stem cells (iPSC) technology has propelled the field of stem cells biology, providing new cells to explore the molecular mechanisms ... See full document

... loss of life quality and premature death. Around 10% of the cases are inherited and ALS8 is an autosomal dominant form of familial ALS caused by mutations in the vamp-associated protein B/C (VAPB) ... See full document

... iPS cells and efficiently differentiate them into specific neuronal subtypes will provide powerful new tools to study complex neurogenetic ...ideal model to study the disease, because they contain ... See full document

... the rat has been an important animal model, which is superior in many respects to the mouse, for example for behavioral, cardiovascular and other physiological ...outbred rat strains are used in ... See full document

... generated from human fibroblasts through retroviral transduction of the four transcription factors Oct4, Sox2, Klf4, and c-Myc ...diameter of 290 m m, 7.6610 3 60.61 cells per hEB) from ... See full document

... embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) have the ability to proliferate indefinitely in an undifferentiated state, and to differentiate to ... See full document

... RNA was extracted using QIAzol reagent (Qiagen Inc., Valencia, CA, USA) according to the manufacturer’s instructions. cDNA was synthesized using a high-capacity cDNA reverse transcription kit (Applied Biosystems, Foster ... See full document

... HFF1 cells were similar to that in untreated HFF1 ...HFF1 cells and GFP-transduced HFF1 cells (Figure ...network of the reprogramming ...subnetworks of the OSKM regulators and ... See full document

... range of sources, including epigenetic memory [5–10,17], inherent differences between IPSCs and ESCs [4,28], artifacts of reprogramming [7] or lab environment ...effects of genetic background have ... See full document

... potential of these immunophenotypically-defined hematopoi- etic progenitors, cells grown for 1 week on OP9 feeder cultures were then grown in methylcel- lulose with hematopoietic cytokines (SCF, IL-3, IL-6, ... See full document

... proportion of cells recovered as multicellular ...concentrations of sodium citrate, 10 to 15 mM, have long been used for non-enzymatic cell dissociation in flow cytometry where the generation ... See full document

... inhibitor of FGFRs, and FGFR-1, FGFR-2 shRNA reduced ES cell cardiomyocyte differentiation, thus demonstrating that the FGF-FGFR signaling pathway is critical in the control of ES/iPS cell differentiation ... See full document

... number of clones were generated using the E6/E8 system, and they stopped growing from the beginning of the third week; further, there was no improvement in cellular growth after switching to E8 ... See full document

... density of 2 × 10 4 cells/well in CnT-07 ...confluent. Cells were incubated with freshly sonicated fluorescent microspheres ...appropriate model for melanosome uptake in epidermal ... See full document

... characterization of MELAS cases is helpful to unveil the still unknown mechanisms and in the search for therapeutic strategies for this ...understanding of mech- anism of mtDNA segregation may be a ... See full document

... induction of cardiomyocytes from hiPSCs hiPSCs (201B6) were differentiated toward cardiomyocytes using modified-directed differentiation protocol (Method, Figure ...differentiation from mesoderm ... See full document