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[PDF] Top 20 The first minutes in the life of a peroxisomal matrix protein

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The first minutes in the life of a peroxisomal matrix protein

The first minutes in the life of a peroxisomal matrix protein

... be the regular pathway? After all, if a single PEX5 molecule could transport an oligomeric protein to the peroxisomal matrix, instead of its subunits, one by one, such a pathway ... See full document

26

The peroxisomal protein import pathway: a functional study of the N-terminal half of PEX5

The peroxisomal protein import pathway: a functional study of the N-terminal half of PEX5

... Shuttling of the peroxisomal protein import receptor PEX5 between the cytosol and the organelle is an ATP hydrolysis requiring process ...that the pathway is regulated and ... See full document

86

The peroxisomal protein import machinery displays a preference for monomeric substrates

The peroxisomal protein import machinery displays a preference for monomeric substrates

... each of these plasmids was transfected alone, revealed a perox- isomal (panel I) and cytosolic (panel II) staining pattern for 2HA-ACOX1 and ACOX1-Flag, ...Interestingly, in a small number of cells ... See full document

13

The peroxisomal matrix protein translocon is a large cavity-forming protein assembly into which PEX5 protein enters to release its cargo

The peroxisomal matrix protein translocon is a large cavity-forming protein assembly into which PEX5 protein enters to release its cargo

... activity of >1000 Ci/mmol; PerkinElmer Life ...used in previous ...using the recombinant protein as a standard and the Tetra- his antibody revealed yields of about 20 ... See full document

40

Ubiquination and deubiquitination in the mammalian pex5-mediated peroxisomal protein import pathway.

Ubiquination and deubiquitination in the mammalian pex5-mediated peroxisomal protein import pathway.

... identity of the E2(s) mediating this ubiquitination event was an immediate ...question. In S. cerevisiae the occurrence of PEX4-dependent monoubiquitination at the conserved ... See full document

91

Characterization of the mechanisms of protein translocation across the mammalian peroxisomal membrane

Characterization of the mechanisms of protein translocation across the mammalian peroxisomal membrane

... proteins in the cytosol yielding stage 1a ...changes the conformation of PEX5 because the receptor only engages the next steps ...with the DTM) if cargo proteins are ... See full document

101

Pex5p, the peroxisomal cycling receptor, is a monomeric non-globular protein

Pex5p, the peroxisomal cycling receptor, is a monomeric non-globular protein

... mechanism, the so-called cycling receptor model, are now numerous (18, 23, ...many of the “details” of this pathway is still quite poor, leaving room for controver- sies between researchers ... See full document

8

A cell-free organelle-based in vitro system for studying the peroxisomal protein import machinery

A cell-free organelle-based in vitro system for studying the peroxisomal protein import machinery

... at the peroxisomal membrane docking/translocation machinery (DTM; Stage 1b) where it becomes inserted (Stage 2) resulting in the release of the cargo protein into ... See full document

37

Development trends of white matter connectivity in the first years of life.

Development trends of white matter connectivity in the first years of life.

... Given the variability of brain anatomy, it is not surprising that anatomical connectivity between regions differs across ...subjects. In this study, we focused on the connections that were ... See full document

15

Matrix Gla Protein expression pattern in the early avian embryo

Matrix Gla Protein expression pattern in the early avian embryo

... role in the formation of the yolk sac angiogenesis since it is expressed from the beginning on a plexus of small caliber sized vessels that will give rise to the vitel- ... See full document

7

Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator

Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator

... imaging of mitotic progression in control (A), Mtor RNAi (B), and Mad2 RNAi ...cation of the time from NEB to ...controls in a pairwise comparison (P = ...from the mean obtained ... See full document

11

The human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.

The human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.

... from the Etablissement Franc¸ais du Sang (Lyon, ...from the light density fraction and T lymphocytes from the high density ...fraction. The purification of monocytes and lymphocytes ... See full document

10

A single peroxisomal targeting signal mediates matrix protein import in diatoms.

A single peroxisomal targeting signal mediates matrix protein import in diatoms.

... family of cellular compart- ments and are widely distributed across the eukaryotic ...within the peroxisomal ...variety of biochemical and metabolic functions [1], which can differ ... See full document

9

The peroxisomal matrix protein import machinery - a PEX5 - centered perspective

The peroxisomal matrix protein import machinery - a PEX5 - centered perspective

... residue of a substrate protein via an isopeptidic bond involving the carboxyl-terminus of ubiquitin and the ε-amine group of that lysine ...monoubiquitination of PEX5 ... See full document

115

Ubiquitin in the peroxisomal protein import pathway

Ubiquitin in the peroxisomal protein import pathway

... ATPases of the REM, PEX1 and PEX6. The mechanistic details of how monoubiquitinated PEX5 and PEX5-related proteins are recognized by the REM are not entirely ...understood. In ... See full document

17

PEX5, the Shuttling Import Receptor for Peroxisomal Matrix Proteins, Is a Redox-Sensitive Protein

PEX5, the Shuttling Import Receptor for Peroxisomal Matrix Proteins, Is a Redox-Sensitive Protein

... by the shuttling receptor PEX5 (9), (ii) this receptor accumulates at the peroxisomal membrane in aging cells (7), and (iii) the export of PEX5 from the peroxisomal ... See full document

15

Protein translocation across the peroxisomal membrane.

Protein translocation across the peroxisomal membrane.

... researchers in the field and, because of its importance in our understanding of Pex14p function, we will take more than just a few words in discussing this ...feeding the ... See full document

18

Pediatrician's knowledge on the management of the infant who cries excessively in the first months of life

Pediatrician's knowledge on the management of the infant who cries excessively in the first months of life

... consisted of an initial piece of identiication information on sex, age, country of residence, time since graduation in Medical School, degree of specializa- tion in Pediatrics ... See full document

6

Ocular alignment during the first seven months of life

Ocular alignment during the first seven months of life

... A posição do reflexo corneano provocado pela fixação de um estímulo luminoso (teste de Hirschberg), posicionado a 1 metro de distância, foi estudada em 273 crianças com idades[r] ... See full document

5

Historical basis of the Miracula of Saint Symeon in Stefan the First-Crowned’s life of Symeon

Historical basis of the Miracula of Saint Symeon in Stefan the First-Crowned’s life of Symeon

... he Life of St. Symeon by Stefan the First-Crowned, heir to the throne of Syme- on-Nemanja, composed during a longer period of time, between 1208 end 1216, con- tains, like ... See full document

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