[PDF] Top 20 Motoneuron differentiation of induced pluripotent stem cells from SOD1G93A mice.

... properties of both ALS-iPS and C57-iPS derived motoneurons in ...RNAs of motoneurons derived from ALS-iPS and C57- iPS cells were extracted using TRIzol (Invitrogen) ... See full document

... demyelination of axons after one ...recruited from the adjacent tissues ...no cells and no vehicle. Cells were transplanted into the chiasm using a 25 m l Hamilton syringe connected to a 30 ... See full document

... these cells through ...35% cells were positive for EGFP expression ...iPS cells [21,22], albeit slower than in their optimal medium ...involvement of oncogene c-MYC during reprogramming might ... See full document

... Induced pluripotent stem cells (iPSCs) are derived from somatic cells by transfecting two pluripotent tran- scription factors, Oct4 (O) and Sox2 (S), and two proto- ... See full document

... rejection of syngeneic mouse iPSCs (miPSCs) following transplantation [17], in which miPSCs derived via the episomal approach were less prone to immune-mediated attack than those generated using viral ...ment ... See full document

... osteoprogenitors from EB-derived cells by isolating tissue-nonspecific alkaline phospha- tase (TNAP)-positive cells using ...that cells separated from EBs did not express TNAP ... See full document

... number of clones. Our experimental design allowed us to test the extent of functional heterogeneity among a large set of iPSCs and to determine if there was an association with genetic ...ability ... See full document

... importance of using serum- free medium for consistent results with riPS cell ...course of our studies another group reported a riPS cell derivation protocol that used both REFs and rat neural precursors as ... See full document

... muscle cells of the host SCID mouse did not express EGFP, muscle cells from rat iPSCs were easily identified in the anterior tibialis muscle ...diameter of the long axis of 100 ... See full document

... One of the essential functions of a keratinocyte is to uptake and transfer ...amounts of mela- nin when incubated with melanosomes, compared to normal ...populations of iPSC-derived keratino- ... See full document

... cell differentiation from ...potential of iPSCs for therapeutic ...endothelial cells, such as BMP4, PD98059, Flt3L, SCF, and VEGF, were selected and divided into groups by function, as shown ... See full document

... pattern of control and ALS8 cells, and it was not possible to identify inclusions in ALS8-derived motor neurons even after the 7 – 8 weeks required for neuronal ...use of the proteasome inhibitor ... See full document

... source of cells for ...original cells reprogrammed; this precludes the need for complex procedures to create the genetic defect and avoids potential secondary effects of the genetic ... See full document

... Induced pluripotent stem (iPS) cells were developed in house [24] as previously described by Takahashi and Yamanaka ...isolated from C57Black6 mice were transduced with ... See full document

... normal differentiation, development and disease [1–3]. Expression of tissue-specific genes, such as Oct-4 [4], Nanog [5], Sry (sex determining region on Y chromosome) [6] and MyoD [7], are induced by ... See full document

... development of retinal neurons from embryonic stem (ES) cell or induced pluripotent stem (iPS) cell cultures ...track stem cell differentiation, fluorescent ... See full document

... iPS Cells: Human induced pluripotent stem cells (iPSCs, cat# SC102A-1) were generated by transducing genetically unmodified human fibroblasts with viruses individually encoding the four ... See full document

... Mouse induced pluripotent stem cells (iPSCs) were first created by overexpression of Oct4, Sox2, Klf4 and c-Myc in somatic cells ...features of embryonic stem ... See full document

... embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) have the ability to proliferate indefinitely in an undifferentiated state, and to differentiate to ... See full document

... expressed from the beginning of the human dermal fibroblasts and their expression was maintained even after receiving the four Yamanaka factors, regardless of the colony type ...and ... See full document