[PDF] Top 20 Rapid detection of multidrug-resistant Mycobacterium tuberculosis using the malachite green decolourisation assay

... Early detection of drug resistance in Mycobacterium tuberculosis isolates allows for earlier and more effective treatment of ...patients. The aim of this study was to ... See full document

... Drug resistant tuberculosis continues to increase and new approaches for its treatment are ...necessary. The identification of M. tuberculosis clinical isolates presenting efflux as ... See full document

... studied the predictive value of four common mycobacterial mutations in strains isolated from endemic areas of ...at the frequency of ...in multidrug-resistant ... See full document

... Limitations of the MTBDRplus assay include detection of resistance to only RIF and INH, and the need for high-level technical skill and infrastructure usually relegating its use ... See full document

... aim of this study was to investigate the performance of a new and accurate method for the detection of isoniazid (INH) and rifampicin (RIF) resistance among Mycobacterium ... See full document

... disease of worldwide distribution, with high morbidity and mortality, mainly in low socio-economic condition ...countries. The state of emergency of tuberculosis caused by the ... See full document

... - The MNRA and REMA were performed with some modifications. Briefly, the MNRA and REMA were performed using a critical concentration of drugs and in liquid ...media. The MGDA and CVDA ... See full document

... to Mycobacterium tuberculosis is a reality worldwide, and its diagnosis continues to be difficult and time ...challenge, the World Health Organization has recommended the use of ... See full document

... Although the nitrate reductase assay is usually per- formed on solid medium (Lowenstein-Jensen medium), Kumar et ...test using the mi- croplate method, similar to our ...els of 99% ... See full document

... shows the sensitivity and specificity obtained with the NRA using NaNO3 or KNO3 compared to the ...sensitivity of 95% with KNO3 and 96% with ...INH the sensitivity was 97% with ... See full document

... Gomes HM, Elias AR, Oelemann MA, Pereira MA, Montes FF, Marsico AG, Kritski AL, Filho LD, Caldas PC, Possuelo LG, Cafrune P, Rossetti ML, Lucena N, Saad MH, Caval- canti HR, Leite CQ, Brito RC, Lopes ML, Lima K, Souza M, ... See full document

... sample of 193 MDR isolates of the R86 genotype from the Eastern Cape and 41 conveniently selected MDR isolates from the Western Cape representing a variety of different Beijing ... See full document

... Republic of Moldova); M Davilovits (Tartu University Lung Hospital, Tartu, Estonia); A Atunes (Vila Nova Gaia/Espinho Medical School, Vila Nova Gaia, Porto University, Porto, Portugal); W de Lange (University ... See full document

... diagnoses of infectious diseases caused by vi- ruses (Fonseca ...for the detection of infectious ...and the bacteria do not need to be viable, which means that inadequately pre- served ... See full document

... as the most specific and widely explored genetic marker (number of IS6110 copies per genome might influence the sensitivity of the ...is the most common biological sample used for ... See full document

... strains of M. bovis BCG were ob- served to be nonreactive by the SD MPT64 ...produce the MPT64 antigen, whereas others, such as BCG Japan, are robust secretors of the antigen (Abe et ... See full document

... number of important issues to account ...reality, the conditional probability that a TB case has MDR-TB when rifampicin resistance is present will vary by ...review, the conditional probability ... See full document

... tems). The reaction was standardised in a total volume of 20 μL, containing 10 μL of the kapa master mix, 1 μL of primers solution and probes (AB Applied Biosystems) at a concentration ... See full document

... out using an Illumina HiSeq 2500 paired-end (100/ 150 bp) ...to the M. tuberculosis H37Rv genome (GenBank Ref. NC000962.3) using BWA-mem and by variant calling using the ... See full document

... Monitoring the extent of and trends in multidrug-resistant tuberculosis (MDR-TB) is a priority of the Brazil- ian National Tuberculosis Control ...Programme. ... See full document