[PDF] Top 20 Reprogramming primordial germ cells into pluripotent stem cells.
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Reprogramming primordial germ cells into pluripotent stem cells.
... EG cells in vitro ...with reprogramming of ...of reprogramming process probably by making histone residues more acetylated and therefore the chromatin accessible to transcription factors that may ... See full document
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Development of a Piggybac based direct reprogramming system for derivation of integration free induced pluripotent stem cells
... Induced pluripotent stem cells (iPSc) have great potential for applications in regenerative medicine, disease modeling and basic ...payload into the host genome and being perfectly excised ... See full document
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Induction of pluripotent stem cells by reprogramming human ocular fibroblasts under xeno-free conditions
... reprogrammed cells can grow into clones with morphologies similar to those of typical ESC clones, visual assessment of iPSC clones is relatively subjective and has a high false-positive ... See full document
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Single cell analysis facilitates staging of Blimp1-dependent primordial germ cells derived from mouse embryonic stem cells.
... bright cells derived from ...(FGF2), Stem Cell Factor (SCF), Leukemia Inhibitory Factor (LIF) and retinoic acid (RA), a driver of PGC proliferation (Figure ...dim cells plated at equivalent numbers ... See full document
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Capture of neuroepithelial-like stem cells from pluripotent stem cells provides a versatile system for in vitro production of human neurons.
... neural stem (NS) cells derived from human fetal cortex ...different pluripotent stem cell lines to give rise spontaneously to neural rosettes following short-term aggregation and subsequent ... See full document
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Few single nucleotide variations in exomes of human cord blood induced pluripotent stem cells.
... 4 cells per well were seeded into a CH-296 (Takara Bio, ...duction, cells were harvested and transferred to 6-well plates pre- seeded with inactivated rat embryonic fibroblast (REF) feeder ... See full document
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MicroRNAs are indispensable for reprogramming mouse embryonic fibroblasts into induced stem cell-like cells.
... somatic cells into iPSCs ...peptide-linked reprogramming factors and a polycistronic doxycycline-inducible lentiviral system ...reprogrammed into induced stem cell-like cells ... See full document
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Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells.
... skin cells with a modified version of our ‘‘Hit and Run’’ lentiviral vector [19] carrying Oct4, Sox2, Klf4 and c-Myc cDNAs driven by the EF-1a ...the reprogramming factors by infection with adenovirus ... See full document
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Neural stem cells achieve and maintain pluripotency without feeder cells.
... reprogrammed into a pluripotent state in a nuclear reprogramming system, such as cell-cell fusion and direct reprogramming ...and pluripotent stem cells ...feeder ... See full document
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Scalable passaging of adherent human pluripotent stem cells.
... human pluripotent stem cells (hPSCs) are labor intensive, result in reduced cell viability and are incompatible with larger scale production necessary for many clinical ...cultures. Cells ... See full document
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Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells.
... differentiation into hepatic cells has not been ...the cells toward ...all cells had detached from the surface within 3 days (Fig ...hiPS cells cultured on R-Fc or NC-Fc maintained the ... See full document
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Differentiation of retinal ganglion cells and photoreceptor precursors from mouse induced pluripotent stem cells carrying an Atoh7/Math5 lineage reporter.
... of cells in the neuroblast layer expressed a lower level of YFP ...Yamanaka reprogramming factors Oct4, Sox2, Klf4, and cMyc, and a lentivirus expressing rtTA ...the reprogramming factors, and cell ... See full document
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Motoneuron differentiation of induced pluripotent stem cells from SOD1G93A mice.
... iPS cells exhibit ES-like properties, we examined some ES cell markers that included alkaline phosphatase (AP) activity and ES cell-specific transcription factors Oct4 and ...iPS cells and the result ... See full document
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The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
... co-culturing cells with BMP4- producing cells as feeders, does not necessarily stimulate PGC production from ...other cells (Eguizabal et ...Efficient reprogramming of ntESCs may cause proper ... See full document
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DNA methylation dynamics in human induced pluripotent stem cells over time.
... The number of passages for ‘‘convergence’’ of the aberrant hyper-methylation seems be dependent on parental cell types and their sex. Disappearance of iPSCs in culture within 10 passages is occasionally observed, ... See full document
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Environmentally induced transgenerational epigenetic reprogramming of primordial germ cells and the subsequent germ line.
... dial germ cells (PGCs) are derived from the epiblast during embryonic development and subsequently migrate to the devel- oping genital ridges ...differentiation into the male or female germ ... See full document
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Physical passaging of embryoid bodies generated from human pluripotent stem cells.
... differentiation into specific lineages, such as neural cells (neurons, oligodendrocytes, and astrocytes), osteoblasts, cardiomyocytes, and endothelial cells by confirming the expression of markers ... See full document
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Examination of a Viral Infection Mimetic Model in Human iPS Cell-Derived Insulin-Producing Cells and the Anti-Apoptotic Effect of GLP-1 Analogue.
... MIN6 cells and in human insulin-producing ...immune cells, in isolated rat beta cells [24, 25], MIN6 cells, and isolated human islets ...beta cells in a viral infectious situation, and ... See full document
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Alchemy and the new age of cardiac muscle cell biology.
... or stem cell and is acquiring a fully differentiated phenotype ...muscle cells, which has been a confounding variable in most other studies of this ...fooled into thinking that the precursor has ... See full document
2
Morphologic and gene expression criteria for identifying human induced pluripotent stem cells.
... retroviruses into the cells by the four retroviral vectors might not be successful, we repeated the analysis by transducing a single polycistronic viral vector encoding Oct3/4- Klf4-Sox2-c-Myc-GFP ... See full document
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