O presente estudo demonstra que, em animais SHAM expostos ao treinamento, as respostas da aorta à Ang II são moduladas por NO de origem endotelial liberado em consequência da ativação de receptores AT2. Já nos animais 2R1C, este mecanismo
modulador endotelial é suprimido, possivelmente pelo elevado estresse oxidativo que é característico deste modelo experimental.
42
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49
APÊNDICES
APÊNDICE A – Composição nutricional da ração consumida "ad libitum" pelos animais estudados
NUVILAB CR-1: Ração para animais de laboratório
Composição básica do produto:
Milho integral moído, farelo de soja, farelo de trigo, carbonato de cálcio, fosfato bicálcico, cloreto de sódio, premix vitamínico mineral e aminoácidos.
Níveis de Garantia por Quilograma do Produto:
Umidade 12,5%, proteína bruta 22,0%, extrato etéreo 4,5%, matéria mineral 10,0%, matéria fibrosa 8,0%, cálcio 1,4% e fósforo 0,8%.
Enriquecimento por Quilograma do Produto:
Vitaminas - vitamina A 25.200,00 UI, vitamina D3 2.100,00 UI, vitamina E 60,00 mg, vitamina K3 12,50 mg, vitamina B1 14,40 mg, vitamina B2 11,00 mg, vitamina B6 12,00 mg, vitamina B12 60,00 mcg, niacina 60,00 mg, ácido pantotênico 112,00 mg, ácido fólico 6,00 mg, biotina 0,26 mg e colina 1.100,00 mg.
Microelementos Minerais - Ferro 50,00 mg; zinco 60,00 mg; cobre 10,00 mg; iodo 2,00 mg; manganês 60,00 mg; selênio 0,05 mg; cobalto 1,50 mg.
Aminoácidos - lisina 100,00 mg; metionina 300,00 mg.
Aditivos:
Antioxidante 100,00 mg.
50
APÊNDICE B – Protocolo de treinamento (10 semanas)
Dias Duração
(min) Aquecimento Endurance Endurance Recuperação
minutos Veloc. Km/h 50% Veloc. máx 60% Veloc. máx minutos Veloc. Km/h 1 15 5 0,3 5 - 5 0,3 2 17 5 0,3 7 - 5 0,3 3 20 5 0,3 10 - 5 0,3 4 25 5 0,3 15 - 5 0,3 5 30 5 0,3 20 - 5 0,3 6 30 5 0,3 20 - 5 0,3 7 30 5 0,3 15 5 5 0,3 8 30 5 0,3 15 5 5 0,3 9 35 5 0,3 15 10 5 0,3 10 40 5 0,3 20 10 5 0,3 11 40 5 0,3 20 10 5 0,3 12 45 5 0,3 25 10 5 0,3 13 45 5 0,3 25 10 5 0,3 14 50 5 0,3 25 15 5 0,3 15 60 5 0,3 30 20 5 0,3 16 45 5 0,3 20 15 5 0,3 17 60 5 0,3 30 20 5 0,3 18 60 5 0,3 30 20 5 0,3 19 60 5 0,3 25 25 5 0,3 20 60 5 0,3 20 30 5 0,3 21 45 5 0,3 15 20 5 0,3 22 60 5 0,3 25 25 5 0,3 23 60 5 0,3 20 30 5 0,3 24 60 5 0,3 15 35 5 0,3 25 60 5 0,3 10 40 5 0,3 26 45 5 0,3 - 35 5 0,3 27 60 5 0,3 - 50 5 0,3 28 60 5 0,3 - 50 5 0,3 29 60 5 0,3 - 50 5 0,3 30 60 5 0,3 - 50 5 0,3 31 30 5 0,5 20 - 5 0,5 32 45 5 0,5 35 - 5 0,5 33 50 5 0,5 40 - 5 0,5 34 55 5 0,5 45 - 5 0,5 35 60 5 0,5 50 - 5 0,5 36 45 5 0,5 35 - 5 0,5 37 60 5 0,5 45 5 5 0,5 38 60 5 0,5 45 5 5 0,5 39 60 5 0,5 40 10 5 0,5 40 60 5 0,5 40 10 5 0,5 41 45 5 0,5 25 10 5 0,5 42 60 5 0,5 40 10 5 0,5 43 60 5 0,5 40 10 5 0,5 44 60 5 0,5 35 15 5 0,5 45 60 5 0,5 30 20 5 0,5 46 45 5 0,5 20 15 5 0,5 47 60 5 0,5 30 20 5 0,5 48 60 5 0,5 30 20 5 0,5 49 60 5 0,5 25 25 5 0,5 50 60 5 0,5 20 30 5 0,5
51
52
APÊNDICE D – Solução nutritiva de Krebs-Henseleit (modificado)
Para preparo de 1000 mL de solução nutritiva de Krebs-Henseleit (modificado):
Sais
[mM]
Peso (g)
NaCl
130
7,61
KCl
4,70
0,35
KH2PO4
1,18
0,16
MgSO4. 7H2O
1,17
0,25
NaHCO3
14,9
1,25
Glicose
11,1
2,00
CaCl2
1,60
0,18
a) Pesar todos os sais, exceto o CaCl2;
b) Em seguida, colocar todos os sais (previamente pesados) em um balão volumétrico de 1000 mL;
c) Completar o volume com água deionizada;
d) Posteriormente, homogeneizar a solução em agitador magnético; e) Adicionar o CaCl2 e homogeneizar novamente;
53
APÊNDICE E - Reatividade Vascular a) Anel de aorta torácica (2mm) b) Transdutor isométrico