Conclusões

Neste estudo, a concentração de forrageira, concentração de peptona, concentração de sulfato de zinco e o tempo de cultivo de P. sanguineus para a produção de FPase, endoglicanase, celobiase e xilanase foram otimizadas, obtendo- se aumentos nas atividades de 4,1, 3,2, 19,5 e 17,2 vezes, respectivamente. Estas enzimas foram então caracterizadas em relação à temperatura e pH, apresentando, de forma geral, maiores atividades em temperaturas entre 45 – 65 °C e em valores de alta termoestabilidade, com t1/2 de 15 e 14,6 h, respectivamente. Já o t1/2 da celobiase foi de 3,1 h. O potencial do extrato otimizado produzido por P. sanguineus em hidrolizar o bagaço de cana pré-tratado com solução alcalina foi avaliado. O maior rendimento de conversão em glicose obtido foi de 25,5 %, quando utilizou-se suplementação com celobiase comercial na proporção de 10:5 (FPase : celobiase). Logo, o extrato enzimático produzido por P. sanguineus mostrou carcterísticas compatíveis com o processo de sacarificação de biomassas lignocelulósicas, contudo,

75 maiores estudos ainda devem ser relizados visando maiores rendimentos de conversão.

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