A New Generation of FRET Sensors for Robust Measurement of Gαi1, Gαi2 and Gαi3 Activation Kinetics in Single Cells.

(B) Representative images of GFP-LC3 fluorescence in Hela cells transfected with GFP-LC3 and treated with DRAM1 siRNAs in the presence or absence of 3-NP (500 m M). Number of cells

Kinetics of the proteolytic cleavage of the FRET-based calpain specific substrate for ACT-treated cells (35 nM toxin), and cells in which m-calpain expression has been silenced

Neste sentido, a presente investigação tem como principal objetivo contribuir para a compreensão da relação entre os domínios laboral e familiar, destacando o

In untreated infected cells (Fig. 2a, b), an intense fluorescence was observed around PV (Fig. 2a – arrow) and dispersed in the cytoplasm of host cell (Fig. 2a – arrowhead); how-

jejuni strains isolated from non-human primates during animal management in the Cecal/Fiocruz, RJ, was able to adhere to or invade HeLa cells.. The difference

As horas extraordinárias são os períodos excedentes à jornada normal contratada, nos quais o empregado permanece à disposição do empregador para a execução de serviços,

were stained for PCNA in the cell nucleus and protein levels were assessed by flow cytometry. Results were analyzed in terms of Geometric Mean of Fluorescence for 20000 cells for

As in WT- cells, the myoB 2 -cells expressing WT-MIB formed waves under a variety of experimental conditions: cells growing in full media (Fig, 2A) and cells starved for up to 3 h