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Romanenko L.A. Age features in protein’s composition modifications of the neurospecifical and glial intermediate filaments in different parts of the red’s brain depend on ionization radiation actions.
Summary. In this work we compare the main changes in protein composition of intermediate filaments due to ionization radiation actions on old and yang rats. The objects of our investigation were 120 rats (60 old and 60 yangs). They were di-vided on groups in depend on ionization radiation action duration – 1 day, 1,2 and 3 week, and the control group. The action of ionization radiationwas modeled with the help of “ -17” in the fate 0,00129 Kl/kg. Brains were divided on parts (cor-tex, cerebellum, gypocamp), homogenized, centrifugised. The quantity of glial and neuronal filaments (NF) was defined by the rocket-lineal immunoelectrophoresis; immunoblotting with specific antibodies was used for qualitative changes defining. After ionization radiation action we observed no age features in protein composition of intermediate filaments. All changes were due to duration of the ionization radiation action. Main changes in neurofilaments were associated with decrease of 210 kDa subunit. Products of degradation (40 - 48 kDa) of fibrillar glial filaments were observed on the 2 week of experiment. There were biphasic changes in glial fibrillar acidic protein in cerebellum and gypocamp of yang and old rats.
Key words: brain, neurofilaments, glial fibrillar acidic protein, ionization radiation.
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Leterrier J.F., Rusakov D.A., Nelson B.D. In-teractions between brain mitichondria and cytoskele-ton: evidence for specialized outer membraine domens involved in the association of cytoskeleton-associted proteins to mitochondria in situ and in vi-tro // Microsc. res. and techn.- 1994.- Vol.27, №2.- P.233-261.
Petkov V.D., Mosorrof A.N., Petkov V.V. Age-related differences in the memory effects of noot-ropic drugs // Acta physiol. pharmacol. Bulgaria.- 1990.- Vol.16, №2.- P.28-32.
Posmantur R.M., Newcomb J.K., Kampfl A. Light and confocal microscopic studies of evolu-tionary changes in neurofilament proteins following cortical impact injury in the rat // Exp. neurol.- 2000.- Vol.161, №1.- P.15-26.
al-__________________________________________________________________________________ І • 2008 • ІI • №2
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solubil-ity and proteolysis // Molec.& clin. neuropathol.- 1995.- Vol.26, №1.- P.1-14.
Towbin H. Immunoblotting an upulate. Bio-chem // Sos. Trans.- 1998.- Vol.16, N4.- P.131.
Yokel R.A., O’Callaghan J.P. An aluminium-induced increase in GFAP is attenuated by some chelators // Neurotoxicol. & teratology.- 1998.- Vol.2, №1.- P.55-60.
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