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Retinal cone photoreceptors require phosducin-like protein 1 for G protein complex assembly and signaling.

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Fig 1. Characterization of the cone photoreceptor-specific PhLP1 knockout mouse. A) PCR genotyping results using the PhLP F primers
Fig 2. Immunolocalization of G t2 subunits in PhLP1-deleted cones. A) Retinal cross-sections from PhLP1 +/+ Cre + and PhLP1 F/F Cre + mice were probed with antibodies specific to Gα t2 , Gβ 3 , Gγ c , and cone  M-opsin and detected with FITC-conjugated sec
Fig 3. Protein expression in retina from PhLP1-deleted cones. A) Immunoblots of whole-retinal extracts for PhLP1, cone G t2 subunits, RGS9-1, Gβ 5 , M-opsin, cone arrestin, and rod G t1 subunits
Fig 4. Immunolocalization of RGS9-1 and Gβ 5 in PhLP1-deleted cones. Retinal cross-sections from PhLP1 +/+ Cre + and PhLP1 F/F Cre + mice expressing EGFP in cones were probed with antibodies to RGS9 (A) or Gβ 5 (B) and detected with AF555-conjugated second
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