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Directly transforming PCR-amplified DNA fragments into plant cells is a versatile system that facilitates the transient expression assay.

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Academic year: 2017

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Figure 2. Transient expression of PCR-fragments in Arabidopsis mesophyll protoplasts. (A) Protoplasts were transfected with plasmid p35S-GFP (Plasmid) or with PCR-fragments 35S-GFP-NOS (PCR-TES)
Figure 3. Comparisons of transformations with plasmid DNA and PCR fragments. (A) PCR-fragments (RD29B-LUC-NOS,  GH3-LUC-NOS and ARR6-LUC-NOS) were transformed into protoplasts,  respec-tively

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