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The role of the methyltransferase domain of bifunctional restriction enzyme RM.BpuSI in cleavage activity.

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Academic year: 2017

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Figure 1.  Oligonucleotide duplexes used in this study and their susceptibility to RM.BpuSI cleavage
Figure 2.  Substrate specificity of RM.BpuSI MTase activity.  (A) In vitro MTase activity
Figure  3.    Effect  of  SAM  and  SIN  on  the  steady  state  kinetics  of  cleavage  activity
Figure  5.    Fluorescence  spectra  of  RM.BpuSI-bound  ANS  as  surrogate  of  protein  conformations
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