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Н і : 29.01.2009
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Pototskaya O.Yu. Three-dimensional computer modeling of birds proepicardium on different stages of embryogene-sis.
Summary. As the proepicardium is the source of many cell populations of the mature heart, including cell components of coronary vessels, its investigation became actual last time. It is known, that there are some differences between birds and mammalian proepicardium; one of them consists in the way, in which they contact to myocardium: mammalian proepicar-dium produce vesicles, which contact to atrioventricular groove, wile in birds there is no vesicles and whole protrusions of proepicardium attach to the heart. But, recent years it became evident, that in rat proepicardium there are no vesicles during all stages of its existence, and in birds there is a proepicardium-like structure producing vesicles, which attach to the heart. Thus, the goal of our research was to characterize changes in the shape of proepicardium during bird’s embryogenesis. We used Cobb 500 chick embryos as a material; on the basis of pictures of proepicardium serial sections, with the help of Photo-shop CS2, Amira for microscopy 5.0, 3ds max 8.0 computer programs, we made three-dimensional models of proepicardium on 15, 16, 17, 18, 21 stages of development by V.Hamburger, H.Hamilton, 1951. The most important changes in proepicar-dium shape observed from 14 to 20 stages of development. During this period proepicarproepicar-dium appeared on the right horn of sinus venous, enlarged and formed several crests, which contacted to atrioventricular groove. Coalescence of these crests led to dorsal mesocardium formation. From 21 to 27 stages of development there were no significant changes in proepicardium shape; the area of its contact to sinus venous was grown downwards while the area of contact to the heart enlarged. No vesi-cles, no “finger-like protrusions” were observed on any stage of bird’s development.
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// . – 2008. – . 2, № 4. – . 37-43.
Differential growth and multicellular villi direct proepicardial translocation to the developing mouse heart / Laurel S. Rodgers, Sofia Lalani, Ray B.
Run-yan, Todd D. Camenisch // Dev. Dyn. – 2008. – Vol.
237. – P. 145–152.
Epicardial development in the rat: a new per-spective / Tresa Nesbitt, Aubrey Lemley, Jeff Davis [et al.] // Microsc. Microanal. – 2006. – Vol. 12. – P. 390–398.
Hamburger V. A series of normal stages in the development of the chick embryo / Viktor Ham-burger, Howard L. Hamilton // J. Morphol. – 1951. - Vol. 88, № 1. – . 49-92.
Martinsen B. J. Reference guide to the stages of chick heart embryology / Brad J. Martinsen // Dev. Dyn. – 2005. – Vol. 233. – P. 1217–1237.
Morphological and molecular left-right
asym-metries in the development of the proepicardium: a comparative analysis on mouse and chick embryos / Inga Schulte, Jan Schlueter, Radwan Abu-Issa [et al.] // Anat. Embryol. (Berl). – 2007. – Vol. 176, № 2. – P. 9-183.
Myocardial heterogeneity in permissiveness for epicardium-derived cells and endothelial precursor cells along the developing heart tube at the onset of coronary vascularization / Heleen Lie-Venema,
Is-mail Eralp, Saskia Maas [et al.] // Anat. Rec. – 2005.
– Vol. 282A. – P. 120–129.
Nahirney P. C. Evidence for an extracellular matrix bridge guiding proepicardial cell migration to the myocardium of chick embryos / Patrick C. Nahirney, Takashi Mikawa, Donald A. Fischman // Dev. Dyn. – 2003. – Vol. 227. – P. 511-523.
Vasculogenesis of the embryonic heart: origin of blood island-like structures / Anna Ratajska, Elz-bieta Czarnowska, Agnieszka Kołodzinska [et al.] // Anat. Rec. – 2006. – Vol. 288A. – P. 223–232.
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