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Borderline ovarian mucinous cystadenoma with invasion of stroma in the goldfish
Carassius auratus (L.)
ARTICLE in BULLETIN- EUROPEAN ASSOCIATION OF FISH PATHOLOGISTS · MARCH 2014
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Ricardo Vieira Rodrigues
Universidade Federal do Rio Grande (FURG) 25PUBLICATIONS 135CITATIONS
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Marcelo Borges Tesser
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Luís André Sampaio
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Available from: Marcelo Borges Tesser Retrieved on: 15 January 2016
* Corresponding author’s e-mail: dcluis@yahoo.com
Borderline ovarian mucinous cystadenoma with
ȱȱȱȱȱęȱ Carassius auratus (L.)
L. A. Romano
1Șǰȱǯȱě
2ǰȱǯȱû
2, R. V. Rodrigues
3, M. B. Tesser
3, M. A. R Garrido-Pereira
1and L. A. Sampaio
31ȱàȱȱȱȱȱȱȱǰȱȱȱęǰȱȱ
ȱȱȱ ǯȱ¡ȱȱŚŝŚǰȱȱşŜǯŘŖŗȬşŖŖǰȱȱ ǰȱǰȱDzȱ2 Programa de Pós- ³¨ȱȱǰȱȱȱȱȱ ǯȱ¡ȱȱŚŝŚǰȱȱşŜŘŖŗȬşŖŖǰȱ
ȱ ǰȱǰȱDzȱ3ȱàȱȱȱȱȱǰȱȱȱęǰȱ
ȱȱȱȱ ǯȱ¡ȱȱŚŝŚǰȱȱşŜŘŖŗȬşŖŖǰȱȱ ǰȱǰȱ
Abstract
ȱȱȱȱ¢ȱȱȱȱęǯȱȱȱȱ¡ȱ
ȱȱȱȱȱȱȱȱ¢ȱȱȱ¡ȱ¢ȱȱȱȱ with basally situated nuclei and abundant intracellular mucins stained with Alcian blue. Sections
ȱȱȱ ȱȱȱȱȱȱȱȱ¢ȱ-
ȱ ȱȱȱȬȱǯȱȱȱȱ ȱȱ
¢ȱȱĴȱȱȱȱŝȱȱȱȱȱ¢ǯȱ
ȱȱȱȱȱ ȱȱȱ ȱȱŝȱ¢ǯȱȱ¢ȱȱ
¢ȱŝȱǻŝǼȱ¡ȱȱȱȱ ȱȱȱȱȱȱě-
ȱȱ ȱȱǰȱȱȱ¡ȱȱȱȱŝȱȱȱ ȱȱȱ
¢ȱȱȱȱȱȱ¢ȱȱ¢ȱȱȱȱȱ¢ǯ
Introduction
ȱęȱȱȱȱȱȱ
ȱȱȱǰȱ ȱȱŘŖǰŖŖŖȱ known species. Their diversity and placement in the phylogenetic tree make them ideal subjects
ȱȱȱǰȱ- ing broader knowledge about basic mecha- nisms, in comparison to studies limited only to mammalian models. Previously, such studies have primarily dealt with tumors in the higher vertebrates (mammals and bird); considerably
ȱĴȱȱȱȱȱȱ ȱ
ȱǻęǰȱǰȱȱǼǯȱ
¢ǰȱȱęȱȱęȱȱ is largely based on mammalian criteria (Ma- sahito et al., 1998; Romano and Marozzi, 2004;
Romano et al., 2010).
ȱȱȱȱȱȱȱȱ in mammals and birds have been reported in ęǯȱȱ¢ȱęǰȱ Ȭȱ
ȱȱȱȱȱęǰȱ- ing viral-induced dermal sarcomas in walleye,
26, Bull. Eur. Ass. Fish Pathol., 34(1) 2014
Stizostedion vitreumȱǻǼǰȱȱĚ¡Ȭ associated hepatocellular adenomas and carci- nomas in rainbow trout, ¢ȱ¢
ǻǼǰȱȱȱȱȱ ȱ¢ȱȱ sporadic tumours in numerous other species ǻȱȱǯǰȱŗşŝŗDzȱǰȱŘŖŗŘǼǯ
Materials and methods
Massive abdominal enlargements were ob-
ȱȱȱȱęǰȱCarassius auratus ǻǯǼȱȱȱȱęȱȱǻȱ
ǰȱǼǯȱȱęǰȱȱ ȱ¢ȱȱ
ǰȱȱȱ ȱȱŚŜŖȱȱȱȱȱȱ
ȱřŚřȱǯȱȱ¢ǰȱȱȱȱŗşȱȱȱ
ȱ ȱȱȱȱȱ¢ǯȱ
ȱȱȱȱ ȱę¡ȱȱŗŖƖȱ
ěȱǰȱȱȱĜǰȱ- tioned at 3 to 5 μm, and stained with haema- toxylin and eosin and alcian blue. In addition histological sections were also stained using immunohistochemical procedures, according
ȱȱęȱȬȬ¡ȱ¡ȱ technique.2 This involved tissue slides being
Ĝǰȱȱ ȱ¡¢ȱȱȱ-
¢ȱ ȱȱȱěȱȱǻ-
ȱǰȱşŖǰȱŞŖǰȱŝŖǰȱśŖƖǼǯȱȱȱ peroxidase activity was blocked by incubating
ȱȱȱŘŖȱȱȱŖǯřƖȱŘŘȱȱȱśƖȱ
ȱǯȱȱ ȱȱȱȱ ȱȱȦŖǯŖśƖȬ ȱŘŖȱǰȱ¢ȱ ȱȱȱȱŗȦŗŖŖȱa, in a 10% PBS bovine serum albumin (BSA) solution
ȱȱȱȱřŖȱȱȱȱȱ
ǯȱȱǰȱȱ¢ȱȬȱ¢-
ȱŝȱǻŝǼȱ¢b was added at a
ȱȱŗDZŘŖŖȱȱȬȱŗŖƖǰȱȱȱȱ were incubated overnight at 41°C in humid
ǯȱȱ ȱ ȱ¢ȱȱȱ
ȱȱŝȱȱȱȱśŖȱȱřŖǯřȬ- obenzidine solution containing 1% PBS-BSA
in 50 mL H2O2c. Counterstaining was then
ȱ ȱ¡¢ȱǻȱȱǯǰȱŗşŞŗǼǯ
Results
The tumour was cystic and multilobulat- ed with abundant mucins (Figure 1). Histo- pathological examination showed the tumour
ȱȱȱȱȱ¢ȱȱ that exhibited typical tall columnar cells with basally situated nuclei and abundant intracellu- lar mucins stained with Alcian blue (Figure 2).
¢ȱ¢ȱȱȱ¢ȱȱ ȱǯȱȱȱ¢ǰȱȱęȱȱ ęȱȱȱ¢ȱȱ-
ȱȱ ȱȱȱĴǯ
A transitional zone was observed between the classical mucinous cystadenoma and papillary
ǯȱȱȱȱȱ¢ȱȱ Ȭě- entiated mucinous cells, the cysts were lined by
¢ȱȱȱ ȱęȱȱ ȱ
ȱȱ¢ǯȱȱȱȱȱ ȱ
ȱȱȱȱȱȱȱ mucinous tumour associated with clusters
ȱȬȱǯȱȱȱ epithelial structures were prominent and the
ȱȱȱȱȱǻȱřȱȱŚǼǯ The immunohistochemical studies showed
ȱ¢ȱȱĴȱȱ
ȱȱŝȱȱȱȱȱ¢- tadenoma. The invasive stromal implant also
ȱȱȱ ȱȱŝȱ¢ȱ (Figure 5).
Discussion
ȱ ȱ ęȱ ȱ ȱ ǰȱ Cyprinus carpio L., are well represented in the
ǯȱȱȱȱȱȱ
ȱȱęȱȱȱ¢ǰȱ¢ȱȱȱ
ȱȱȱǯȱ ęȱȱȱ ȱ
Figure 1.ȱ ȱȱȱȱȱ¢ȱȱȱȱ¢ȱ¢ȱ ȱȱ walls with abundant mucins. Bar = 1 cm. Insert: Carassius auratus with massive abdominal enlargements.
Bar = 4.5 cm
Figure 2. Mature cyst showing typical tall columnar cells with basally situated nuclei and abundant intracellular mucins (arrow) supported connective tissue (SC). Haematoxylin and eosin. Bar = 200 μm
28, Bull. Eur. Ass. Fish Pathol., 34(1) 2014
Figure 3.ȱ ȱ ȱ ȱ ȱ ȱ ¢ȱ ȱ ȱ ¢ǯȱ ȱ ȱ
ȱȱȱȱ¢ȱȱ ȱȱȱȱȱǻ Ǽǯȱ¡¢ȱ and eosin. Bar = 50 μm
Figure 4.ȱȱȱ¢ȱȱȱ¢ȱ ȱ¢ȱȱ¢ȱȱ ǻ Ǽȱȱȱȱȱ¢ȱȱȱȱȱǻȘǼȱ¡¢ȱȱǯȱȱƽȱŗŖŖȱ μm
adapted to environments that are suboptimal
ȱȱȱȱęǯȱȱȱȱȱ
ȱȱȱ¢ȱȱ¡¢ȱȱȱ damage caused by toxins that may be carcino-
ǯȱȱ¢ȱȱȱȱȱȱěȱ
ȱ¡ȱȱ¢ȱ¡ȱȱęȱ
ȱȱȱȱȱȱǯ
Abdominal enlargement associated with ovarian neoplasia was previously described in eight 4-6-year-old adult carp living in breeding ponds
ȱȱęȱȱȱȱǻǰȱŘŖŖśǼǯȱ
ȱȱ ȱ¢ȱęȱȱ
¢ȱȱȱȱ¢ǰȱ- bly teratoid in nature. Another study described 21 ovarian neoplasias in ornamental hybrid
ȱǻ ȱȱ¢ǰȱŗşŝŞǼǯHistologi- cally, these neoplasias exhibited considerable
ǰȱȱ¢ȱ ȱȱ¢ȱȱ
ȱ¢ȱȱȱȱȱȱȱȱ dysgerminoma, granulosa or theca cell tumors
or embryonic carcinomas. Squamous cell nests ȱȱ ȱ¢ȱȱȱȱ tumor, but no mucinous cystadenoma was ob- served. Thus, ovarian tumours in carp may have diverse histogenetic origins and most likely
ȱȱȱȱȱȱȱȱ mesenchyme.
ȱȱ¢ǰȱȱěȱȱ-
ȱȱȱȱȱ¢ȱȱ primary ovarian mucinous tumours (atypical
ȦȱȱǼȱȱȱ
Ĝȱȱȱȱȱȱ- phology.
ȱ¢ȱȱȱ¢ȱȱȱ epithelial origin and represent a unique inter- mediate stage between benign cystadenomas
ȱǯȱ¢ȱȱȱȱ
¢ȱ¢ȱȱȱȱȱ¢ȱ
ȱȱȬȱȱȱ¢ȱȱ Figure 5.ȱȱ ȱȱȱȱȱ¡ȱŝȱ¡ȱǻ ǼǯȱȬŝȱ ȱ haematoxylin counterstain. Bar = 100 μm
30, Bull. Eur. Ass. Fish Pathol., 34(1) 2014
ȱȱȱȱǯȱȱ
ȱ¢ȱȱȱȱȱ
ȱȱȱȱȱǻǰȱ 2005).
The World Health Organization (WHO) classi- ęȱȱȱȱȱȱȃȱ tumours”; they have been too widely recognized
ȱȱȱȱȃ ȱȱȄȱ
ȱȱȃȱȱǰȄȱ- nology that was accepted by the WHO in the
¢ȱŘŖŖŖǯȱ¢ǰȱȱȱȱȱȱ ȃȱȱ¢ȱȱ ȱȱ-
ȄȱȱȱȱǻĵǰȱȱǯǰȱŘŖŖŖǼǯȱ
ȱȱȱȱĚȱȱ
ȱ ȱ ȱ ȱ ěȱ ȱ primary ovarian mucinous tumours and meta- static mucinous carcinomas in the human ovary.
ȱ¢ȱȱ¢ȱŝȱǻŝǼȱ¡ȱȱ restricted and allows ovarian mucinous tumors
ȱȱěȱȱ ȱȱ
ǰȱȱȱ¡ȱȱȱȱŝȱȱ
ȱ ȱȱȱ¢ȱȱȱȱ
ȱȱ¢ȱȱ¢ȱ-
ȱȱȱȱ¢ǯȱ¢ȱȱ
ȱȱ ȱ¢ȱ ¢ȱ-
¢ȱȱȱȱŝȱǻşŞƖǼȱǻȱ 2011). Ovarian neoplasms have been reported in ęǰȱȱȱȱ¢ȱȱ
ȱ¢ȱ ȱȱȱȱȱǰȱ
ȱȱ ȱȱȱȱȱęȱȱ case (Hart, 2005).
Acknowledgments
ȱȱ ȱȱȱ¢ȱ -
ȱȱ£ȱȱȱȱȱ-
ȱȱȱǯȱěǰȱǯȱûǰȱ E.P. Gusmão, M.A. Garrido-Pereira. L.A.L.A.
Romano and L.A. Sampaio, who received pro-
¢ȱȱ ȱȱȱ£-
ȱȱȱǰȱȱǻȱȱ ȱřŖŗŖŖŘȦŘŖŗŘȬŜȱȱřŖŞŖŗřȦŘŖŖşȬřǼǯȱ
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