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Inhibition of TDP-43 aggregation by nucleic acid binding.

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Academic year: 2017

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Figure 2. The effects of ssDNA and ssRNA on TDP-43 solubility. (A) The supernatant (S) and pellet (P) of TDP-43, incubated at 37 u C for 3 h, in the absence (control) or presence of various ssDNA [i.e
Figure 3. The solubility of wild-type, mutant (F147L/F149L), and N-terminally truncated (TDP 208–414 ) TDP-43 in the absence or presence of ssDNA or ssRNA
Figure 4. Characterization of the refolded TDP-43 protein. (A) The refolded wild-type and double-mutation (F147L/F149L) TDP-43 identified with coomassie blue in the SDS-PAGE, or by western blotting with TDP-43 antibody
Figure 5. Confocal immunofluorescent images of Neuro2a cells of TDP-43 variants. (A) Inclusions were observed only in the mutant (F147L/F149L, indicated by yellow arrow) and truncated (TDP 208–414 , indicated by red arrow) TDP-43 rather than the wild-type

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