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Derivation, characterization, and stable transfection of induced pluripotent stem cells from Fischer344 rats.

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Academic year: 2017

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Figure 1. Generation of rat iPS (riPS) cells. (A) Rat embryonic fibroblasts (REFs) (left), primary riPS cell colony derived thereof after transduction with lentiviruses expressing Oct4, Klf4, Sox2, cMyc, and EGFP with some satellite colonies (middle), and
Figure 2. Pluripotency of generated riPS cells. (A) Immunostain- Immunostain-ing of cultured riPS cells for Nanog (left) (63x objective, confocal imaging, clone IVC2), Oct4 (middle, clone IIIB9), and SSEA1 (right, clone G4) (20x objective, fluorescent micr
Figure S1 Lentivirus silencing in undifferentiated riPS cells. (A) riPS cell clone IIIB9 after 22 passages (p22) in culture.

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