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Gene cloning, expression, and characterization of the Bacillus amyloliquefaciens PS35 lipase

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Academic year: 2019

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Figure 1 - Phylogenetic tree of C-4 lipase. M13 primers were used for nucleotide sequencing, and the forward and reverse sequences were aligned using ClustalX 1.83
Figure 3 - SDS-PAGE of crude and purified lipase preparations. Lane M: molecular weight markers, 1 and 2: crude samples, 3 and 4: load samples, 5 and 6: purified lipase (pool I) from the phosphocellulose column, 30 and 50 mL samples.
Figure 4 - Effect of pH on the lipase activity and stability. Citrate, phos- phos-phate, and Tris-HCl buffers (100 mM) were used for the acidic, neutral, and alkaline ranges, respectively
Table 3 - Effect of organic solvents and chemical modifiers on the lipase activity.
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