CURRICULUM VITAE
L
UIS
F.S.
G
OULÃO
P
ERSONAL
D
ATA
Full Name:
Luís Filipe Sanches Goulão
Nationality: PortugueseBirth Place: Carnaxide, Oeiras Date of Birth: July, 1973 Institutional
Address: Tropical Research Institute [Instituto de Investigação Científica Tropical – IICT, IP] BioTrop-Agri4Safe, Pólo Mendes Ferrão
Pavilhão de Agro-Indústrias e Agronomia Tropical (3º piso) Tapada da Ajuda, 1349-017 Lisboa – PORTUGAL
Phone: +351 213 617 247
E-mail: goulao@iict.pt
E
DUCATION
2006 - PhD IN AGRONOMIC ENGINEERING – Postharvest Physiology and Molecular Biology of Fruits
TECHNICAL UNIVERSITY OF LISBON (SCHOOL OF AGRONOMY; ISA/UTL), LISBON - PORTUGAL
1999 - MSci IN SUSTAINABLE AGRICULTURE AND HORTICULTURE (Scholar Part)
TECHNICAL UNIVERSITY OF LISBON (SCHOOL OF AGRONOMY; ISA/UTL), LISBON – PORTUGAL
1996 - DEGREE IN AGRONOMIC ENGINEERING – Plant Breeding
TECHNICAL UNIVERSITY OF LISBON (SCHOOL OF AGRONOMY; ISA/UTL), LISBON – PORTUGAL
R
ESEARCH
I
NTERESTS
- Agricultural RESEARCH FOR DEVELOPMENT (AR4D) and innovation in AGRIbusiness - FOOD AND NUTRITIONAL SECURITY AND SAFETY (hunger and "hidden hunger") - COLLABORATIVE RESEARCH with CPLP countries
E
XPERTISE: PLANT SCIENCES APPLIED TO ECONOMICALLY IMPORTANT CULTIVATED SPECIES - Postharvest biology and technology: fruit ripening, quality and storage- Domestication and exploitation of native tropical fruits and vegetables towards food and nutritional security - Molecular, biochemical and enzymatic modifications of the plant cell wall with agronomic/industrial relevance - Applications of molecular markers in plant management, breeding and agriculture
- Identification, expression and functional characterization of genes and metabolites (-OMICs) with agronomic and industrial relevance in economically important plant species
T
RAINING IN
I
NTERNATIONAL
L
ABORATORIES
2008 - Visiting scholar at the Department of Genetics - University of Georgia, Athens, GA - USA (supervision by Prof. Chung-Jui Tsai and Dr. Scott Harding), September-November
2004 – Visiting scholar at the Department of Biology - Pennsylvania State University, PA – USA (supervision by Prof. Daniel Cosgrove), March-June
A
DVANCED
T
RAINING
2012 – European Cooperation in Science and Technology (COST) Course: "Next Generation Sequencing Data Analysis with Chipster”, 13-15 June, Helsinki, Finland
2010 –The Gulbenkian Training Programme in Bioinformatics Course: “Automatic Annotation and Data Mining”, 13-14 May, Oeiras, Portugal
2009 – The Gulbenkian Training Programme in Bioinformatics Course: “Workflows and Programatically Accessible Tools”, 16-17 November, Oeiras, Portugal
2009 – The Gulbenkian Training Programme in Bioinformatics Course: “Microarray Data Analysis using R and Bioconductor”, 14-18 September, Oeiras, Portugal
2008 –European Science Foundation for Functional Genomics (ESFFG) Workshop: "Advanced large scale gene expression profiling – focus on miRNAs, ChIP-on-chip and whole genome sequencing", 19-23 May, Turku, Finland
2002 – Workshop "Coconut genetic resources management using microsatellite kit and dedicated statistical software", 15-24 April, Montpellier, France
S
CIENTIFIC
A
PPOINTMENTS
SINCE JUL 2009: Associate Researcher (“Investigador Auxiliar”) at the Tropical Research Institute (IICT), Oeiras.
Head of the Agri4Safe Group, Lisbon, since Oct 2012
NOV 2006 - JUN 2009: Post-Doctoral Fellow at the Plant Cell Wall Group – Instituto de Tecnologia Química e
Biológica (ITQB/UNL), Oeiras
JAN 2005 - OCT 2006: Graduate Student at the Plant Cell Wall Group - Instituto de Tecnologia Química e Biológica
(ITQB), Oeiras
JUL 2004 - MAR 2005: Research Grantee for Program AGRO 8.1 at the Technical University of Lisbon (School of
Agronomy; ISA/UTL), Lisbon / ‘Painho’ Fruit Storage Central, Cadaval
JUL 2000 - JUL 2004: PhD Student at the Horticulture Department - Technical University of Lisbon (School of
Agronomy; ISA/UTL), Lisbon
JAN 1997 - DEC 1999: “Young Graduate Researcher” Fellow at the Technical University of Lisbon (School of
Agronomy; ISA/UTL), Lisbon / University of Algarve (Faculty of Engineering and Natural Resources), Faro / National Institute for Fruit Research ‘Vieira Natividade’ (ENFVN, IP), Alcobaça
MAR 1996 - DEC 1996: Trainee at the Department of Forage Crops - National Institute for Plant Breeding (ENMP,
IP), Elvas
M
EMBER OF
E
XPERT
G
ROUPS AND
T
ASK
F
ORCES
SINCE APR 2014 – High Level Policy Dialogue Expert Working Group on “Science, Technology and Innovation for
Food and Nutrition Security and Sustainable Agriculture” in EU-Africa cooperation, European Commission
SINCE JAN 2013 – IICT Representation in the Secretariado do Grupo de Trabalho do Ensino Superior e de
Instituições de Investigação (GT-ESII) to support the implementation of the CPLP’s Food and Nutritional Security
Strategies
P
ARTICIPATION
IN
F
INANCED
R
ESEARCH
P
ROJECTS
SINCE JUN 2014: Fundo Nacional de Investigação (FNI) de Moçambique: Avaliação frutícola e nutricional e potencial
de conservação de frutos nativos para valorização e segurança nutricional. [Project Coordinator at IICT]
SINCE JUN 2014: Fundo Nacional de Investigação (FNI) de Moçambique: Promoção da valorização de espécies da
floresta de miombo moçambicano no contexto do bio-empreendedorismo.
SINCE FEB 2014: German Federal Enterprise for International Cooperation (GIZ): Comparing two fire management
strategies in the Limpopo Tourism and Parks: creating the foundations for a fire management system in the Limpopo National Park.
SINCE JUL 2013: PTDC/AGR-PRO/3386/2012: Implications of environmental changes of CO2 and temperature at the
plant and bean quality levels in the coffee tree (Coffea spp.) - multidisciplinary characterization of the impact and acclimation ability of the producing species.
SINCE JUL 2013: IFAP - ProDeR 4.2.2 - PA nº 46511: RTDT Competitividade Internacional – Rede de Tratamento e
Difusão de Tecnologia com vista à Competitividade Internacional do Tecido Empresarial Nacional.
SINCE FEB 2013: International Rice Research Institute (IRRI): FrutosNativos4SAN – Mozambique [Program
SINCE JAN 2013: Camões, Instituto da Cooperação e da Língua (CICL): Formação Avançada de Recursos Humanos
na Área de Biotecnologia aplicada à gestão e monitorização de recursos naturais – Fase II.
SINCE JAN 2013: Fundo Aberto – Universidade Eduardo Mondlane (Fundo de Investigação Científica; FIC): Análise
ecológica e molecular para avaliação do estado de conservação da população de chanfuta (Afzelia quanzensis Welw.) da floresta de Michafutene (Marracuene, Maputo).
SINCE JAN 2013: Fundo Aberto – Universidade Eduardo Mondlane (Fundo de Investigação Científica; FIC):
Mapeamento e diversidade genética de Hypoxis spp. (batata africana) e Warburgia salutaris (chibaha) na província de Maputo.
SINCE MAR 2012: PTDC/AGR-GPL/116923/2010: VitiShade - Abscission of seedless grapes: shedding the genes from
the shade. [Project Coordinator]
SINCE JAN 2012: PTDC/AGR-ALI/118477/2010: SafeFruit - Aureobasidium pullulans, a biofungicide for the control of
postharvest decay in pears: insights into its action mechanisms.
SINCE JAN 2012: IFAP - ProDeR 4.1 - PA nº 45261, partnership #449: AROMA4SAFE [Project Coordinator at IICT]
SINCE JAN 2012: IFAP - ProDeR 4.1 - PA nº 23923, partnership #373: PROdUVA [Project Coordinator at IICT]
JAN 2011 – DEC 2012: PUBLIC-PRIVATE PARTNERSHIP IICT/GEOCAPITAL HOLDINGS (Macau): Sustainable
biodiesel production from Jatropha curcas in countries of the CPLP (Portuguese-speaking countries).
DEC 2009 - DEC 2011: BIOVERSITY INTERNATIONAL F06: Bridging the divide between natural resource conservation strategies and the role of key forest genetic resources in Niassa National Reserve, Mozambique.
NOV 2007 – DEC 2010: PTDC/AGR-AAM/64078/2006: Molecular evaluation and characterization of tolerance
mechanisms to adverse environmental conditions in Coffea sp. - central role of the oxidative stress control at leaf level.
JAN 2005 – JUN 2009: POCTI/AGR/46671/02: Cell wall proteins with roles in xylogenic programmes in eucalyptus. JAN 2004 - MAR 2005: AGRO 518: Improving postharvest quality and safety of pear, apple and kiwi.
JUL 2000 - JUN 2004: POCTI/33733/99: Genetic expression and activity of non-pectolytic enzymes and
involvement in apple physical properties.
2002: CIRAD/COGENT (France): Improving coconut production in Mozambique through germplasm
characterization and evaluation of promising hybrids. (Project canceled in 2003 due to cancelling of funding)
JAN 1997 - DEC 1999: PRAXIS/3./3.2/HORT/2163/95: Bio-molecular characterization of Portuguese cultivars of
apple and pear.
JAN 1997 - DEC 1999: PRAXIS/3/3.2/HORT/2143/95: Sanitation and breeding of Portuguese fruit tree cultivars
using a biotechnological approach.
JAN 1997 - DEC 1999: PRAXIS/3/3.2/HORT/2136/95: Study of the biology and sexual reproduction in the chestnut
and almond trees: Genetic and fruit breeding.
1996: PAMAF 3088: Study of annual pastures under different soil conditions: annual alfalfas and sub-clovers.
S
UPERVISING
E
XPERIENCE
(at graduate level only) PhD THESIS:SINCE JAN 2011 – Sara Nobre Gonçalves Domingos. Thesis: “Thinning via shading in seedless table grapes: Unravelling the physiological and molecular basis of berries abscission” (Co-orientation with Prof. Cristina Moniz Oliveira (ISA/UTL); grant:SFRH/BD/69076/2010)
SINCE JAN 2010 – João Carlos Martins Fernandes. Thesis: “Mineral stress response of Vitis cell wall transcriptome: Identification, characterization and expression of genes from key families involved in wall biosynthesis and modification” (Co-orientation with Prof. Sara Amâncio (ISA/UTL); grant: SFRH/BD/64047/2009)
MSci THESIS:
SINCE DEC 2013 – Filipa Neto. Thesis: “Fighting food insecurity in Mozambique: nutritional evaluation of a blend of dehydrated native vegetables used as a food supplement” (Co-orientation with Prof. Margarida Moldão (ISA/UL - MSci in Food Engineering))
SINCE OCT 2013 – Déjan Bogosavljevic. Thesis: “Evaluation of different production and drying methodologies in the aromatic quality of Stevia rebaudiana” (Co-orientation with Prof. Margarida Moldão (ISA/UL - MSci in Food Engineering))
SINCE SEP 2013 – César Castelo Branco Esteves. Thesis: “Insights on the mechanisms of anthracnose tolerance in physic nut (Jatropha curcas)” (Co-orientation with Prof. Ana Lúcia Leitão (FCT/UNL - MSci in Food Technology and Safety))
SINCE SEP 2013 – Maria João Prata. Thesis: “Assessment of ‘Rocha’ pear cell wall modifications as a defense mechanism against postharvest molds, under biocontrol” (Co-orientation with Prof. Maria Teresa Rebelo (FC/UL – MSci in Human Biology and Environment))
SINCE JUL 2013 – Sara Martins Paiva. Thesis: “Evaluation of oligosaccharides in fruit postharvest storage potential” (Co-orientation with Prof. Ana Lúcia Leitão (FCT/UNL - MSci in Food Technology and Safety))
JAN 2012 – JUL 2013 – Ivete Sandra Maquia. Thesis: “Molecular characterization of forest genetic resources from the Miombo woodlands at the Niassa National Reserve: development of barcode- and ISSR- markers”, Eduardo Mondlane University, Mozambique (Co-orientation with Dr. Ana Ribeiro (IICT) – MSci in Biotechnology)
JAN 2012 – JUL 2013 – Elias Manuel Amadeu Militão. Thesis: “Genetic characterization of Afzelia quanzensis Welw. populations from the Michafutene forest”, Eduardo Mondlane University, Mozambique (Co-orientation with Dr. Ana Ribeiro (IICT) – MSci in Biotechnology)
CAPACITY BUILDING:
JAN 2014 – MAY 2014 – Burcu Belge (Visiting Scholar; PhD student from the University of Lleida, Spain): “Quantification of the expression of cell wall- and cuticle-modifying genes in response to postharvest treatments in peach and cherry”
APR 2013 – MAR 2014 – Vânia Alexandra da Silva Cardoso (Researcher; grant associated with the VitiShade project): “Searching for molecular signaling of flower/fruitlet abscission in Vitis vinifera”
JUL 2013 – SET 2013 – Miguel Fraga (Summer trainee; Student of Biotechnology from the Universidade da Beira Interior): “qrt-RT-PCR quantification of MAP(K)n transcription factors and hormone receptors and their effect in regulating floral abscission triggered by different stimuli”
JUL 2013 – SET 2013 – Guilherme Tonial Neves (Summer trainee; Student of Biotechnology from the Universidade Federal dos Grandes Dourados - BRAZIL / Universidade da Beira Interior): “Identification and expression of genes related with anthracnose tolerance in Jatropha curcas, a biodiesel producing species”
AUG 2012 – DEC 2012 – Ana Cláudia Raposo (Researcher; grant associated with the VitiShade project): “Agronomic, physiological and molecular assessment of seedless grapes induced for flower/fruitlet abscission under field conditions”
FEB 2011 – JUL 2012 – Vanessa Alexandra Victoriano dos Santos (Degree in Biotechnology; Universidade Lusófona de Humanidades e Tecnologias (ULHT)): “Molecular characterization of the IICT’ Jatropha curcas germplasm collection”
JAN 2011 –AUG 2011 – Juliana Barbosa (PhD student; Lavras Federal University (UFLA), Brazil): “Association of coffee quality with physical and chemical parameters of the green bean”
DEC 2010 – Celestino Uamusse (Researcher, Center for Research and Development in Ethonobotany, Mozambique): "Basic molecular biology and bioinformatics methods" (co-orientation with Dr. Ana Ribeiro and Dr. Ana Fortunato (IICT))
L
ECTURING
In Portugal:2007-2012: Two lab classes / year (Biophysics of the primary cell wall) in “Plant Biology” classes of the Degree in
Biology; 3rd semester (ISA/UTL, Lisbon)
2012: Two lectures in “Genetic Engineering” classes of the Degree in Plant Biotechnology; 3rd semester (Coimbra
Agronomy School, Coimbra)
2011: Lecture “The paradoxical characteristics of the primary cell wall” in the Workshop “Hidden Stories Within Cell
Walls” (ISA/UTL, Lisbon)
2010 – 2011: One lab class in “Methods of Molecular Analysis” classes of the MSci in Functional Biology (ISA/UTL)
2010: One lecture in “Modified Organisms” classes of the MSci in Biofuels; 1st semester (Coimbra Agronomy School,
Coimbra)
2004: One lecture in “Plant Development” classes of the Degree in Agronomic Engineering; 9th semester (ISA/UTL)
2001 – 2004: Assistant in “Plant Biotechnology” lab classes of the Degree in Agronomic Engineering; 6th semester
(ISA/UTL)
In Portuguese Speaking Countries (CPLP):
SINCE 2011: Invited Lecturer at the Eduardo Mondlane University (UEM), Maputo, Mozambique - Master (MSci) in Biotechnology:
SINCE 2011: Transcriptomics and Metabolomics: Module CB10: “Systems Biology”
2014: Measurement of Biodiversity and Genetic Structure - Concepts and Calculations: Module CB07:
“Genetic Characterization Techniques”
2013: Theoretical and practical workshop “Quantification of gene expression using quantitative real-time PCR” (Centre of Biotechnology (CB) - Eduardo Mondlane University (UEM), Maputo, Mozambique), 20-21 February.
E
VALUATOR IN
I
NTERNATIONAL
P
ROJECT
C
ALLS
2011 AND 2012 - French National Research Agency (Agence Nationale de la Recherche; ANR). Committee: "Interface Biology-Physics, Interface Biology-Chemistry and Biotechnology".
C
ONSULTING AND
A
DVISORY FOR THE
P
RIVATE
S
ECTOR
:
OCT-DEC 2013 – Consulting for the study of the banana value-chain in Benguela and Baía-Farta, Angola (promoter: World Bank / Fundo de Apoio Social; Programa de Desenvolvimento Local) - “Estudo da Cadeia de Valor da Banana nos Municípios de Benguela e Baía Farta - Província de Benguela”; Programa de Desenvolvimento Local; Consultors: Ana Portugal Melo, Carolina Bastos, Cristina Vaz Tomé and Luís Filipe Goulão. 75 pp.
2009-2012 – Scientific advisory on the implementation of a "Coffee Science Centre” in Campo Maior (promoter: Grupo Nabeiro)
J
URY
P
ARTICIPATION
IN
A
CADEMIC
D
EGREES (at graduate level only)
PhD DISSERTATIONS:2014 – Inês Maria Castelhano Graça Andrade. Dissertation: “Análise funcional de uma quitinase da classe III (CgCHI3) e de uma glutationa S-transferase (CgGST), envolvidas na simbiose entre Casuarina glauca e
Frankia” - PhD in Biochemistry (Minors: Biotechnology) – FCT/UNL, Costa de Caparica, 4th July (5th
opponent).
2010 – José Mário Leitão Ribeiro. Dissertation: “The importance of extensin network formation in the definition of primary cell wall properties” - PhD in Biological Sciences – ITQB/UNL, Oeiras, 29th January (3rd opponent).
MSci DISSERTATIONS:
2013 – Hugo Paulo Vale Nóbrega. Dissertation: “Utilização de redes de ensombramento para monda de bagos em uva de mesa” - MSci in Agronomy – ISA/UL, Lisbon, 27th December (Main opponent).
2013 – Lucinda Alculete Lopes de Araújo Feitio. Dissertation: “The importance of ubiquitin binding for a novel group for endosomal trafficking protein in Arabidopsis thaliana” - MSci in Biotecnology – CB/UEM, Maputo, Mozambique, 19th July (Main opponent).
2012 – Frederic Cobb. Dissertation: “Effect of irrigation on berry and skin cell wall composition in the grape varieties ‘Touriga Nacional’ and ‘Trincadeira’” - MSci Vinifera EuroMaster, European Master of Sciences of Viticulture and Oenology – ISA/UTL, Lisbon, 4th December (Opponent).
2012 – Carlos André Silva Correia. Dissertation: “Flower and berry thinning by shading in ‘Sugraone’ and ‘Crimson Seedless’ table grapes” - MSci in Agronomy – ISA/UTL, Lisbon, 16th March (Main opponent).
O
RGANIZATION
OF
S
CIENTIFIC
M
EETINGS
2014 – Co-coordinator and Member of the Organizing and Scientific Committees of the: “1st NATIONAL WORKSHOP
ON NATIVE FRUIT CROPS” to be held on November, Maputo, Mozambique.
2012 – Member of the Organizing Committee of the: “MINERAL IMPROVED CROP PRODUCTION FOR HEALTHY
FOOD AND FEED: ENHANCED NUTRITIONAL VALUE OF PLANT-DERIVED FOOD OR FEED” (3rd Annual Workshop -
COST Action FA0905. All WGS+MC Meeting), 23-25 October, Lisbon, Portugal.
2012 – Member of the Scientific Committee of the "MOZAMBIQUE INTERNATIONAL CONGRESS: HISTORY, MEMORY AND SCIENCE”, 24-26 October, Lisbon, Portugal.
2006 – Member of the Local Organizing Committee of the "PEROXIDASE 2006 CONGRESS", 6-9 July, Aveiro, Portugal.
E
DITORIAL
A
CTIVITY IN
S
CIENTIFIC
J
OURNALS
MEMBER OF THE EDITORIAL BOARD:
SINCE 2012: International Scholarly Research Notices, Hindawi Publishing Corporation (termed “ISRN Agronomy,
2011-2014: International Journal of Plant Physiology and Biochemistry (IJPPB), Academic Journals. PEER REVIEWER:
108 manuscripts (MS) to date: Scientia Horticulturae - 49 MS; Food Control – 9 MS; Journal of Agricultural and
Food Chemistry – 5 MS; HortScience – 2 MS; Carbohydrate Polymers – 5 MS; PLOS ONE – 2 MS; Plant Cell Tissue and Organ Culture – 2 MS; Tree Physiology – 2 MS; Functional Plant Biology – 1 MS; Phytochemistry – 1 MS; Industrial & Engineering Chemical Research – 1 MS; Journal of Food Engineering – 1 MS; Journal of the American Society of Horticultural Sciences - 1 MS; Journal of Horticultural Science & Biotechnology – 1 MS; Sustainability – 1 MS; Journal of Biotechnology – 1 MS; Industrial Crops and Products – 2 MS; Plant Systematics and Evolution – 1 MS; The Scientific World Journal: Agronomy – 3 MS; ISRN Agronomy – 11 MS; African Journal of Biotechnology – 1 MS; Journal of Biological and Food Science Research – 1 MS; International Journal of Food, Agriculture and Environment – 1 MS; International Journal of Plant Physiology and Biochemistry – 1 MS; Emirates Journal of Food and Agriculture – 1 MS; African Journal of Agricultural Research – 1 MS; Universitas Scientiarum – 1 MS; Journal of Tropical Forest Science – 1 MS
S
CIENTIFIC
A
SSOCIATIONS
M
EMBERSHIP
:
International Society for Horticultural Science (ISHS; member no. 67561)
Portuguese Biochemical Society / Portuguese Society of Plant Physiology (SPB; member no. 3274)
Portuguese Society of Horticulture (Associação Portuguesa de Horticultura - APH; member no. 1954 - Secretary of
the Directive Board since May 2013) Professional networks:
International Tropical Fruits Network (TFNet) The Postharvest Education Foundation (TEF)
IdCPLP: Rede de Investigação e Desenvolvimento na CPLP Plataforma de Biotecnologia de Plantas da CPLP
Liga de Amigos do Jardim Botânico Tropical (LAJBT; member no. 70)
P
RIZES AND
A
WARDS
1998 – Prize “D. Dinis” in Agronomy Sciences. Granted by the “Prof. Agostinho da Silva” Fundation / “Montepio Geral” Bank
G
RANTS AND
F
ELLOWSHIPS
JUNE 2012: Travel Bursary SEQAHEAD COST BM1006 ACTION
NOV 2006 – JUN 2009: Post-Doctoral Grant SFRH/BPD/17565/2004 – FCT
MAY 2008:
JUL 2004 – MAR 2005: Short Visit & Exchange Grant Researcher EUROPEAN SCIENCE FOUNDATION AGRO 8.1 (AGRO 518)
JUL 2000 – JUN 2004: PhD Student SFRH/BD/1133/2000 – FCT
L
IST
OF
P
UBLICATIONS
:
THESIS:
Goulão LF (2005). Identification, cDNA cloning and expression, and enzymatic activity of proteins involved in
ripening and softening of apple (Malus x domestica Borkh. cv ´Mondial Gala´) fruits. PhD Thesis. Instituto Superior de Agronomia, Lisbon, 269 pp.
Goulão LF (1996). Caracterização de germoplasma de Trifolium subterraneum L. Degree in Agronomic
Engineering Thesis. Instituto Superior de Agronomia, Lisbon, 87 pp.
BOOK CHAPTERS:
Goulao LF and Oliveira CM (2014). “Multilocus profiling with AFLP, ISSR and SAMPL” In: Molecular Plant Taxonomy: Methods and Protocols (Ed.: P. Besse). Methods in Molecular Biology Series, Vol. 1115,
Humana Press Inc, Springer Publishing, USA. pp 211-231 (ISBN 978-1-62703-766-2)
Goulao LF, Fernandes JC, Lopes P and Amâncio S (2012). "Tackling the cell wall of the grape berry". In: The Biochemistry of the Grape Berry (Ed.: H. Gerós, M.M. Chaves and S. Delrot). Bentham Science Publishers.
pp. 172-193 (eISBN: 978-1-60805-360-5)
Goulao LF, Almeida DP and Oliveira CM (2010). "Effect of enzymatic reactions on texture of fruits and vegetables".
In: Enzymes in Fruit and Vegetable Processing: Chemistry and Engineering Applications (Ed.: A. Bayindirli). CRC Press, USA. pp. 71-122 (ISBN: 9781420094336)
PAPERS IN INTERNATIONAL SCIENTIFIC PERIODICALS WITH REFEREE: (ISI h-index = 11)
Guenha R, Salvador BV, Rickman J, Goulao LF, Muocha IM and Carvalho MO (2014). Hermetic storage with plastic sealing to reduce insect infestation and secure paddy seed quality: a powerful strategy for rice farmers in Mozambique. Journal of Stored Products Research (DOI: 10.1016/j.jspr.2014.06.007)
Lara I, Belge B and Goulao LF (2014). The fruit cuticle as a modulator of postharvest quality. Postharvest Biology
and Technology 87: 103–112.
Maquia I, Muocha I, Naico A, Martins N, Gouveia M, Andrade I, Goulao LF and Ribeiro AI (2013). Molecular, morphological and agronomic characterization of the sweet potato (Ipomoea batatas L.) germplasm collection from Mozambique: genotype selection for drought prone regions. South African Journal of
Botany 88: 142-151.
Ramalho JC, Fortunato AS, Goulao LF and Lidon FC (2013). Cold-induced changes in mineral content in leaves of
Coffea spp.: Identification of descriptors for tolerance assessment. Biologia Plantarum 57: 495-506.
Fernandes JC, García–Angulo P, Goulao LF, Acebes JL and Amâncio S (2013). Mineral stress affects the cell wall composition of grapevine (Vitis vinifera L.) callus. Plant Science 205–206: 111-120.
Maquia I, Ribeiro N, Silva V, Bessa F, Goulao LF and Ribeiro AI (2013). Genetic diversity of Brachystegia boehmii Taub. and Burkea africana Hook. f. across a fire gradient in Niassa National Reserve, northern Mozambique. Biochemical Systematics and Ecology 48: 238–247.
Sousa PMF, Videira MAM, Bohn A, Hood BL, Conrads TP, Goulao LF and Melo AMP (2012). The aerobic respiratory chain of Escherichia coli: from genes to supercomplexes. Microbiology 158: 2408–2418.
Goulao LF, Fortunato AS and Ramalho JC (2012). Selection of reference genes for normalizing quantitative
real-time PCR gene expression data with multiple variables in Coffea spp. Plant Molecular Biology Reporter
30: 741–759.
Goulao LF, Vieira-Silva S and Jackson PA (2011). Association of hemicellulose- and pectin-modifying gene
expression with Eucalyptus globulus secondary growth. Plant Physiology and Biochemistry 49: 873-381.
Goulao LF, Cosgrove DJ and Oliveira CM (2008). Cloning, characterisation and expression analyses of cDNA clones
encoding cell wall-modifying enzymes isolated from ripe apples. Postharvest Biology and Technology 48: 37-51.
Goulao LF and Oliveira CM (2008). Cell wall modifications during fruit ripening: when a fruit is not the fruit. Trends in Food Science & Technology 19: 4-25.
Goulao LF, Santos J, Sousa I and Oliveira CM (2007). Patterns of enzymatic activity of cell wall-modifying enzymes
during growth and ripening of apples. Postharvest Biology and Technology 43: 307-318.
Goulao LF and Oliveira CM (2007). Molecular identification of novel differentially expressed mRNAs up-regulated
during ripening of apples. Plant Science 172: 306-318.
Carvalho L, Goulão L, Oliveira C, Gonçalves J and Amâncio S (2004). RAPD assessment for identification of clonal identity and genetic stability of in vitro propagated chestnut hybrids. Plant Cell Tissue and Organ Culture
77: 23-27.
Goulão L and Oliveira C (2001). Molecular characterisation of cultivars of apple (Malus x domestica Borkh.) using
Goulão L, Cabrita L, Oliveira C and Leitão J (2001). Comparing RAPD and AFLP markers in discrimination and
estimation of genetic similarities in apple (Malus x domestica Borkh.) cultivars. Euphytica 119: 259-270. Monte-Corvo L, Goulão L and Oliveira C (2001). ISSR analysis of cultivars of pear and suitability of molecular
markers for clone discrimination. Journal of the American Society for Horticultural Sciences 126: 517-522.
Goulão L, Valdiviesso T, Santana C and Oliveira C (2001). Comparison between phenetic characterisation using
RAPD and ISSR markers, and phenotypic data of cultivated chestnut (Castanea sativa Mill.). Genetic
Resources and Crop Evolution 48: 329-338.
Goulão L, Monte-Corvo L and Oliveira C (2001). Phenetic characterization of plum cultivars by high multiplex
ratio markers: Amplified Fragment Length Polymorphisms and Inter-Simple Sequence Repeats. Journal
of the American Society for Horticultural Sciences 126: 72-77.
Oliveira C, Mota M, Monte-Corvo L, Goulão L and Silva D (1999). Molecular typing of Pyrus based on RAPD markers. Scientia Horticulturae 79: 163-174.
INVITED REVIEWS AND OPPINION PAPERS:
Goulao LF (2010). Pectin de-esterification and fruit softening: revisiting a classical hypothesis. Stewart Postharvest Review 6. pp. 1-12(12).
PAPERS IN INTERNATIONAL CONFERENCE PROCEEDINGS:
Domingos S, Scafidi P, Oliveira CM, di Lorenzo R and Goulao LF (2013). Effects of light reduction at bloom on fruit set in ‘Black Magic’ table grape cv in early and late production cycles. Journal of Viticulture and Enology (Ciência e Técnica Vitivinícola) 28: 1001-1005. (with Oral Communication)
Ramalho JC, Rodrigues AP, Semedo JN, Costa C, Pais I, Fortunato AS, Palos I, Batista-Santos P, Goulao LF, Maia I, Scotti-Campos P, Máguas C, Nesi A, Damatta FM and Lidon FC (2013). Impact of long-term increased ambient CO2 on the photosynthetic functioning of Coffea arabica and C. canephora leaves. Proceedings of
the 24th International Conference on Coffee Science, ASIC (Association for Science and Information on Coffee) 2012, pp. 705-710. (CD-ROM) (with Oral Communication)
Goulao LF (2012). Regulation of postharvest fruit softening: taking pictures off the wall. In: Avances en Poscosecha de frutas y hortalizas (Ed.: I. Recasens, J. Graell and G. Echeverría). Lleida. pp. 101-114 (ISBN:
978-84-695-4683-3). (with Plenary Oral Communication)
Batista-Santos P, Goulão LF, Partelli F, Campos PS, Pais IP, Fortunato AS, Ribeiro AI and Ramalho JC (2011). The effect of low temperature on metabolism of membrane lipids in Coffea sp. and associated gene expression. Proceedings of the ASIC (Association for Science and Information on Coffee) 2010: The 23rd
International Conference on Coffee Science, pp. 428-436. (CD-ROM) (with Poster)
Goulão LF and Oliveira CM (2005). Patterns of temporal mRNA expression and enzyme activity of pectolytic and
non-pectolytic cell-wall degrading enzymes in growth and ripening of apple fruits. Acta Horticulturae
682: 91-98. (with Oral Communication)
Monte-Corvo L, Goulão L and Oliveira C (2002). Discrimination of pear cultivars with RAPD, AFLP, and ISSR. Acta
Horticulturae 596: 187-191. (with Poster)
PAPERS IN NATIONAL SCIENTIFIC PERIODICALS WITH REFEREE:
Goulão L, Monte-Corvo L, Mota M, Silva D and Oliveira C (1999). Caracterização de cultivares de macieira (Malus
pumila Mill.) e de pereiras (Pyrus spp.) por amplificação aleatória de DNA (RAPD). Revista de Ciências
Agrárias XXII(2): 65-82.
Farinha N, Brito A, Goulão L and Carneiro J (1999). Avaliação de linhas avançadas de Trifolium subterraneum L. em selecção nas condições de Elvas. Pastagens e Forragens 20: 145-152.
Goulão L, Farinha N, Tavares-de-Sousa M and Martins J (1999). Caracterização morfológica de linhas de Trifolium
subterraneum L. com base em análise multivariada. Pastagens e Forragens 20: 63-74.
PAPERS IN NATIONAL CONFERENCE PROCEEDINGS:
Ribeiro AI and Goulao LF (2013). Agrobiodiversidade: oportunidades e desafios para a resolução de problemas agro-ambientais e promoção do desenvolvimento socio-económico. Actas da Conferência do Sector Agrário e Desenvolvimento Rural em Moçambique: Transformação Estrutural e Competitividade do Sector Agrário (in press)
Domingos S, Pesquita C, Couto F, Goulao LF and Oliveira C (2011). Improving Gene Functional Analysis: a Study on Ethylene-induced Leaf Abscission Using the Gene Ontology and ProteInOn. INForum 2011 – Atas do Terceiro Simpósio em Informática (Ed.: R. Barbosa, L. Caires), pp. 72-77. (with Oral Communication)
Fortunato AS, Goulao LF, Leitão AE, Rodrigues AD, Jackson P, Partelli FL, Ribeiro AI and Ramalho JC (2011). The role of the antioxidantive system in Coffea sp. acclimation to simultaneous chilling and drought stress. VII Simpósio de Pesquisa dos Cafés do Brasil (CD-ROM), 22-25 August, Araxá, MG, Brazil, 6 pp (with Poster)
Alpalhão A, Neto C, Goulão L, Clemente J, Henriques J, Lourenço I e Oliveira CM (2009). Efeito do 1-metilciclopropeno em pêra ‘Rocha’ em diferentes estados de maturação armazenadas em atmosfera normal. Actas Portuguesas de Horticultura 11: 224-230. (with Poster)
Dionísio L, Neto C, Goulão L, Clemente J, Henriques J, Lourenço I e Oliveira CM (2009). Efeito do 1-metilciclopropeno em maçãs ‘Royal Gala’ armazenadas em atmosfera normal. Actas Portuguesas de
Horticultura 11: 162-171. (with Oral Communication)
Neto C, Goulão L, Clemente J, Gomes MH, Duarte P, Almeida D e Oliveira C (2005). Efeito do 1-metilciclopropeno em maçãs e pêras armazenadas em atmosfera normal. Actas Portuguesas de Horticultura 8: 382-389. (with Poster)
Goulão L and Oliveira CM (2004). Identification, genetic expression and enzymatic activity of ripening and
softening related proteins in apple fruits. Maturação e Pós-Colheita: Frutos e Hortícolas (Ed.: MG Barreiro), INIAP/EAN. pp. 145-149. (with Oral Communication)
C
OMMUNICATIONS
1:
INVITED PLENARY COMMUNICATIONS:
Ribeiro AI, Neves L, Goulao LF, Zacarias A, Melo AMP and Ribeiro N (2014). Enhancing scientific knowledge impact in Mozambique. AGRINATURA Science Days: “Growing Together: Family Farming and Agricultural Sciences transforming World Food Systems”, May 5-7, Vienna, Austria
Goulao LF, Vaz Tomé C, Leitão AE, Martins AC and Melo AMP (2013). Saber Tropical, Sabor Tropical. 53º Encontro
dos Descobrimentos: A Indústria do Doce no Mercado Internacional, November 14-15, Lisbon, Portugal
Goulao LF and Melo AMP (2013). O IICT na Investigação e Cooperação para a Segurança Alimentar na CPLP.
Conferência “Segurança alimentar e desafios da saúde em África” - Dia de África 2013; 50º Aniversário da União Africana, Grupo de Embaixadores Africanos, May 25, Lisbon, Portugal [Oral Presentation by L. Goulao]
Goulao LF (2013). Biotechnology towards a productive agriculture in challenging climatic environments. II
International Seminar on Biotechnology: Opportunities of Economic and Social Development in Valorization and Sustainable Management of Bioresources. May 22-24, Maputo, Mozambique
Goulao LF (2013). Research progresses and challenges in “biodiversity to food and nutritional security” towards
climate-smart agriculture in Mozambique. Policy Workshop on Climate Change Adaptation for Agriculture in Mozambique: Challenges for Agriculture Research, February 28, Maputo, Mozambique
Goulao LF (2012). Regulation of postharvest fruit softening: taking pictures off the wall. POST LLEIDA 2012 - VII
Iberian Postharvest Symposium. October 1-4, Lleida, Spain.
Ribeiro A and Goulao LF (2012). Biodiversidade e desenvolvimento sustentável. Cluster “Tecnologias Tropicais”: Biologia e Medicina Tropicais, AITECOeiras, May 29, Oeiras, Portugal
ORAL COMMUNICATIONS:
Domingos S, Nóbrega H, Cardoso V, Goulao LF and Oliveira CM (2014). Utilização de redes de ensombramento para monda de flores em uva de mesa ‘Thompson Seedless’ e ‘Crimson Seedless’. 3º Simpósio Nacional de Fruticultura, to be held 4-5 December, Vila Real, Portugal [accepted; ; to be presented by C. Oliveira] Domingos S, Nóbrega H, Cardoso V, Ramalho JC, Oliveira CM and Goulao LF (2014). Effect of shade and gibberellic
acid applied during bloom on fruit set in Thompson and Crimson Seedless table grape cultivars. 7th International Table Grape Symposium, to be held 11-14 November, Mildura, Victoria Australia. [accepted; to be presented by S. Domingos]
Belge B, Goulao LF, Comabella E, Graell J and Isabel Lara (2014). Postharvest heat and CO2 shocks induce changes
in cuticle composition and cuticle-related gene expression in ‘October Sun’ peach fruit. POST’14 VALENCIA - VIII Iberian Maturation and Postharvest Symposium. October 21-23, Valencia, Spain. [to be presented by I. Lara]
Fernandes JC, Cobb F, Tracana S, Costa G, Valente I, Goulao LF and Amâncio S (2014). Relating water stress conditions to grape berry skin mechanical properties and cell wall composition and expression of remodeling genes. 2nd Annual Meeting of the COST FA 1106 “Fruit Quality”, to be held 22-24 September, Chania, Greece. [accepted; to be presented by S. Amâncio]
Goulao LF, Barbosa F, Khan M, Maquia I, Mula H, Muocha I, Mussane CRB, Ribeiro N, Senkoro A e Ribeiro AI
(2014). Conferência de Investigação da Universidade Eduardo Mondlane to be held September 18-19, Maputo, Mozambique [submitted; Oral Presentation by L. Goulao]
Domingos S, Cardoso V, Leitão AE, Nóbrega H, Sánchez C, Ramalho JC, Oliveira CM and Goulao LF (2014). Thinning in seedless table grapes: triggering abscission by different signals acts according to the same mechanisms? The 29th International Horticultural Congress: Sustaining Lives, Livelihoods and Landscapes, to be held 17-22 August, Brisbane, Australia. [accepted; to be presented by L. Goulao]
Ribeiro-de-Barros AI, Saide J, Maquia I, Senkoro A, Muocha I, Barbosa F, Goulao LF, Neves L and Ribeiro N (2014). Strengthening national capacity in Agrobiotechnology: a collaborative platform between Mozambique and Portugal. Fourth RUFORUM Biennial Meeting, to be held July 21-25, Maputo, Mozambique [accepted; Oral Presentation by A. Ribeiro]
Maquia I, Goulão LF, Ribeiro N and Ribeiro A (2014). Aplicação de marcadores moleculares para avaliação da biodiversidade florestal nas matas de miombo: estudo de caso na Reserva Nacional de Niassa, Moçambique. Conservation Science in Mozambique Workshop: To preserve Mozambique’s amazing biodiversity for future generations. April 21-22, Maputo, Mozambique [Oral Presentation by I. Maquia] Militão E, Maquia I, Mazivile M, Chirinzane C, Goulão LF, Ribeiro N and Ribeiro A (2014). Avaliação da
variabilidade genética de chanfuta (Afzelia quanzensis welw.) na floresta de Michafutene: implicações para os programas de conservação da espécie. Conservation Science in Mozambique Workshop: To preserve Mozambique’s amazing biodiversity for future generations. April 21-22, Maputo, Mozambique [Oral Presentation by E. Militão]
Domingos S, Scafidi P, Oliveira CM, di Lorenzo R and Goulao LF (2013). Effects of light reduction at bloom on fruit set in ‘Black Magic’ table grape cv in early and late production cycles. The 18th International Symposium GiESCO (Group of international Experts of vitivinicultural Systems for CoOperation) 2013, 7-11 July, Porto, Portugal. [Presented by S. Domingos]
Marques VR, Melo AMP and Goulão LF (2013). Riscos associados à pobreza e à transição nutricional em países da CPLP. Fórum Conselhos Científicos dos Laboratórios de Estado – Ciclo de Encontros “A Ciência na Prevenção e Mitigação dos Riscos em Portugal – Riscos e Segurança Alimentar”, January 31, Lisbon, Portugal [Oral Presentation by V. Marques]
Ramalho JC, Rodrigues AP, Semedo J, Pais IP, Batista-Santos P, Goulao LF, Fortunato AS, Palos IM, Maia I, Scotti-Campos P, Damatta FM and Lidon FC (2012). Impact of long-term increased ambient CO2 on Coffea arabica and C. canephora photosynthetic functioning. ASIC (Association for Science and Information on
Coffee) 2012: The 24th International Conference on Coffee Science – Programme and Abstracts, 11–16 November, San José, Costa Rica. pp. 232 [Presented by J. Ramalho]
Goulao LF, Bila R, Mula HC, leitão AE, Grundy I, Broli A, Mwangwela A, Kingsley M, Penicela L and Ribeiro AI
(2012). Postharvest handling and conservation of indigenous fleshy fruits from southeastern Africa: first steps of the NutrIndiFruit project. Book of Abstracts of the International Congress Tropical Knowledge in Mozambique: history, memory and science, October 24-26, Lisbon, Portugal. pp. 65 [Oral Presentation by L. Goulao]
Maquia I, Goulão LF, Ribeiro N and Ribeiro AI (2012). Caracterização molecular de recursos genéticos florestais das matas de miombo na Reserva Nacional de Niassa: desenvolvimento de códigos de barras e marcadores ISSR. Book of Abstracts of the International Congress Tropical Knowledge in Mozambique: history, memory and science, October 24-26, Lisbon, Portugal. pp. 68 [Oral Presentation by I. Maquia] Militão E, Maquia I, Mazivile M, Goulão L and Ribeiro AI (2012). Caracterização da diversidade genética da
população de chanfuta (Afzelia quanzensis Welw.) da floresta de Michafutene. Book of Abstracts of the International Congress Tropical Knowledge in Mozambique: history, memory and science, October 24-26, Lisbon, Portugal. pp. 69 [Oral Presentation by E. Militão]
Senkoro A, Barbosa F, Manjate A, Silva V, Maquia I, Muocha I, Moura I, Goulão L and Ribeiro A (2012). Estudo e conservação de plantas medicinais em Moçambique. Book of Abstracts of the International Congress Tropical Knowledge in Mozambique: history, memory and science, October 24-26, Lisbon, Portugal. pp. 62 [Oral Presentation by A. Senkoro]
Fernandes JC, García-Angulo P, Goulao LF, Acebes JL and Sara Amâncio (2012). Mineral stress alters cell wall cellulose and pectin methylesterification in grapevine (Vitis vinifera L.) callus. NutriPlanta 2012 - XIV Simposio Hispano-Luso de Nutrición Mineral de las Plantas, 23-26 July, Madrid, Spain. pp. 81 [Presented by J. Fernandes]
Ribeiro A, Muocha I, Maquia I, Goulão LF, Melo AMP, Senkoro A, Barbosa F, Ribeiro N, Saide J and Neves L (2012) Research & Education in Agrobiotechnology: a partnership between IICT & UEM. II Cooperation and Education (COOPEDU): Africa and the World, July 5-6, Lisbon, Portugal. pp. 15 [Oral Presentation by A. Ribeiro]
Ribeiro A, Muocha I, Maquia I, Goulão LF, Melo AMP, Senkoro A, Barbosa F, Ribeiro N, Saide J, Neves L (2012). IICT (Portugal) & UEM (Mozambique): a consolidate partnership in agrobiotechnology. Livro de Atas: Ensino Superior e Investigação Científica no Espaço da CPLP, Ed. AULP, pp- 305-311. (with Oral Communication by A. Ribeiro in the XXII Encontro da Associação das Universidades de Língua Portuguesa (AULP), June 18-20 in Maputo, Mozambique. pp. 54)
Lidon FC, Ramalho JC, Scotti-Campos P, Pais IP, Leitão AE, Almeida AS, Maçãs B, Coutinho JP, da Silva MMA, Duarte MP, Fernando AL, Ribeiro AI, Goulão LF, Simões F, Costa AR, Matos J, Reboredo FH (2012). The Portuguese project of wheat biofortification in Fe and Zn, COST Action FA0905. Programa e Resumos do Simpósio: Desenvolver culturas mais nutritivas – Biofortificação de trigo, 6 March, Elvas, Portugal. pp. 13 [Presented by F. Lidon] (+ 4 supporting posters)
Ribeiro A, Goulão LF, Melo AMP, Palos I and Ramalho J (2012). Colaboração interinstitucional para promoção da biotecnologia em Moçambique. IICT International Conference, Science in the Tropics: glimpsing the past, projecting the future, January 5-7, Lisboa, Portugal. pp. 25 [Oral Presentation by A. Ribeiro]
Lidon FC, Leitão AE, Goulão LF, Ribeiro A and Ramalho JC (2012). Seriação de metodologias destinadas à produção de farinha de trigo biofortificada em Fe e Zn para populações carenciadas de países em vias de desenvolvimento. IICT International Conference, Science in the Tropics: glimpsing the past, projecting the future, January 5-7, Lisboa, Portugal. pp. 29 [Oral Presentation by F. Lidon]
Domingos S, Pesquita C, Couto F, Goulao LF and Oliveira C (2011). Improving Gene Functional Analysis: a Study on Ethylene-induced Leaf Abscission Using the Gene Ontology and ProteInOn. INForum 2011 - Simpósio em Informática (Bioinformatics Track - N66), September 8-9, Coimbra, Portugal. pp. 72-77 [Presented by S. Domingos]
Fernandes JC, García-Angulo P, Goulao LF, Acebes JL and Amâncio S. (2011). Is there a correlation between primary cell wall (CW) composition and the expression of key CW biosynthesis and modification genes in grapevine callus under mineral stress? XII Congreso Hispano-Luso de Fisiologia Vegetal, June 21-24, Castelló de la Plana, Spain. pp. 33 [Presented by J. Fernandes]
Ribeiro AI, Leitão AE, Silva MJ, Santos MR, Pinto MM, Domingues AM, Melo A, Goulão LF, Santos PC, Batista-Santos P, Fortunato AS, Graça IM, Silva S, Sousa P and Ramalho JC (2010). Agricultural Research for Development: A contribution of Eco-Bio/IICT. Livro de Actas do 1º Worshop Anual BioPlant, March 29-30, Porto, Portugal. pp.18 [Oral Presentation by A. Ribeiro]
Dionísio L, Neto C, Goulão L, Clemente J, Henriques J, Lourenço I, Oliveira CM (2006). Efeito do 1-metilciclopropeno em maçãs ‘Royal Gala’ armazenadas em atmosfera normal em dois anos consecutivos de ensaio. I Simpósio Nacional de Fruticultura: Programa e Resumos das Comunicações, 12-13 October, Alcobaça, Portugal. pp.49 [Presented by C. Neto]
Goulão L and Oliveira CM (2004). Identification, genetic expression and enzymatic activity of ripening and
softening related proteins in apple fruits. IV Iberian Symposium on Maturation and Postharvest 2004, 6-9 October, Oeiras, Portugal. pp. 145-146-9 [Presented by L. Goulão]
Goulão L and Oliveira CM (2004). Patterns of temporal mRNA expression and enzyme activity of pectolytic and
non-pectolytic cell-wall degrading enzymes in growth and ripening of apple fruits. 5th International Postharvest Symposium, 6-11 June, Verona, Italy. pp. 6 [Presented by L. Goulão]
Goulão L, Monte-Corvo L and Oliveira C (1999). Identification of European and Japanese plum cultivars based on
ISSR (Inter-Simple Sequence Repeats) markers. Resumos do 2º Workshop de Biotecnologia de Plantas, 6-7 May, Faro, Portugal. pp.21 [Presented by L. Goulão]
Farinha N, Carneiro J, Brito A and Goulão L (1998). Avaliação de linhas avançadas de Trifolium subterraneum L. em selecção nas condições de Elvas. Programa e Resumos da XIX Reunião de Primavera da Sociedade Portuguesa de Pastagens e Forragens, May, Castelo Branco, Portugal. pp.12. [Presented by N. Farinha]
POSTERS IN CONFERENCES:
Domingos S, Ramalho A, Nóbrega H, Leitão AE, Goulao LF and Oliveira CM (2014). Qualidade e conservação da cultivar de uva de mesa ‘Crimson Seedless’. 3º Simpósio Nacional de Fruticultura, to be held 4-5 December, Vila Real, Portugal (accepted)
Belge B, Goulao LF, Comabella E, Graell J and Lara I (2014). A role for non-pectolytic proteins in postharvest firmness loss in sweet cherry? POST’14 VALENCIA - VIII Iberian Maturation and Postharvest Symposium. October 21-23, Valencia, Spain (accepted)
Maquia I, Goulão L, Ribeiro N and Ribeiro A (2014). Estudo da biodiversidade florestal das matas de miombo na Reserva Nacional de Niassa: aplicação de marcadores moleculares ISSR e códigos de barras. Conferência de Investigação da Universidade Eduardo Mondlane to be held September 18-19, Maputo, Mozambique Mula HC, Bila CR, Khan M, Zacarias F, Penicela L, Maridalho P and Goulao LF (2014). A multi-approach
programme to promote native fruits consumption in Mozambique. The 29th International Horticultural Congress: Sustaining Lives, Livelihoods and Landscapes, to be held 17-22 August, Brisbane, Australia. (accepted)
Maquia I, Goulão LF, Ribeiro, NS, Ribeiro-de-Barros AI (2014). Application of molecular markers for biodiversity and taxonomy studies in legume tree species from the miombo woodlands, Niassa Natioanl Reserve, Mozambique. Fourth RUFORUM Biennial Meeting, to be held July 21-25, Maputo, Mozambique (accepted)
Sánchez C, Cristóvão MM, Nunes A, Prata MJ, Ferreira-Pinto MM; Santos M, Vasilenko P, Coimbra MA, Barreiro MG and Goulao LF (2014). Understanding the antagonistic mechanisms triggered by the biological control agent Aureobasidium pullulans in `Rocha´ pears against postharvest blue mold decay. XII International Pear Symposium, to be held 14-18 July, Leuven, Belgium (accepted)
Domingos S, Cardoso V, Nóbrega H, Leitão AE, Ramalho JC, Oliveira C and Goulao LF (2014). Molecular induction of flower abscission in Vitis vinifera L. as a response to carbon shortage and to exogenous gibberellic acid. The Plant Biology Europe FESPB/EPSO 2014 Abstracts Book, 22-26 June, Dublin, Ireland. pp. 381.
Goulao LF, Mula HC and Bila CR (2013). Native fruits towards food and nutritional security in Mozambique. First
International Conference on Global Food Security, September 29-October 2, Noordwijkerhoul, Netherlands. P2.16
Victoriano-Santos V, Fortunato A, Santos M, Cardoso V, Ramalho JC, Silva MJ and Goulao LF (2013). A drought-related dehydrin in the biodiesel-producing species Jatropha curcas L.: A molecular signature at the leaf level. FV2013: XIII Congresso Luso-Espanhol de Fisiologia Vegetal – Abstract Book (N. Vieira, N. Saibo and M.M. Oliveira, Eds.), SPFV, 24-28 July, Lisbon, Portugal. pp. 392.
Domingos S, Scafidi P, Cardoso V, Leitão, AE, DiLorenzo R, Oliveira CM and Goulao LF (2013). Molecular signatures of floweret abscission signaling in Vitis vinifera. FV2013: XIII Congresso Luso-Espanhol de Fisiologia Vegetal – Abstract Book (N. Vieira, N. Saibo and M.M. Oliveira, Eds.), SPFV, 24-28 July, Lisbon, Portugal. pp. 159
Cristóvão MM, Goulao LF, Pinto MF, Domingos S and Sánchez C (2013). Postharvest biocontrol in ‘Rocha’ pear using an antagonistic yeast-like fungus against blue mold: Insights from expression of candidate genes. FV2013: XIII Congresso Luso-Espanhol de Fisiologia Vegetal – Abstract Book (N. Vieira, N. Saibo and M.M. Oliveira, Eds.), SPFV,, 24-28 July, Lisbon, Portugal. pp. 309
Ramalho JC, Rodrigues AP, Semedo JN, Pais I, Martins LD, Tomaz MA, Fortunato AF, Palos I, Batista-Santos P, Costa C, Goulao L, Leitão AE, Lopes E, Ribeiro A, Silva MJ, Rodrigues C, Maia R, Máguas C, Reboredo F, Morais LE, Sanglard LM, Araújo WL, Scotti-Campos P, DaMatta FM and Lidon FC (2013). Reinforcement of Photosynthetic Components in Coffee Plants Under Long-Term High Growth CO2 Counteracts
Down-Regulation. FV2013: XIII Congresso Luso-Espanhol de Fisiologia Vegetal – Abstract Book (N. Vieira, N. Saibo and M.M. Oliveira, Eds.), SPFV, 24-28 July, Lisbon, Portugal. pp. 330
Ramalho JC, Semedo JN, Pais I, Scotti-Campos P, Rodrigues AP, Fortunato AF, Leitão AE, Lopes E, Palos I, Silva MJ,
Goulao L, Batista-Santos P, Ribeiro A, Costa C, Martins LD, Tomaz MA, Rodrigues C, Maia R, Máguas C,
Reboredo F, Lidon FC, Sanglard LM, Morais LE, Araújo WL and DaMatta FM (2013). Coffee Plants Displayed Absence of Photosynthetic Down-Regulation Under Long-Term High Growth CO2. FV2013: XIII
Congresso Luso-Espanhol de Fisiologia Vegetal – Abstract Book (N. Vieira, N. Saibo and M.M. Oliveira, Eds.), SPFV, 24-28 July, Lisbon, Portugal. pp. 329
Scotti-Campos P, Pais I, Semedo JN, Martins LD, Maia I, Reboredo F, Simões-Costa MC, Lopes E, Tomaz MA, Rodrigues AP, Batista-Santos P, Fortunato AF, Palos I, Goulao L, Leitão AE, Ribeiro A, Silva MJ, Rodrigues C, Maia R, Máguas C, Nesi A, morais L, Martins SC, daMatta FM, Lidon FC and Ramalho JC (2013). Impact of long term high CO2 on membrane stability in three coffee genotypes. FV2013: XIII Congresso
Luso-Espanhol de Fisiologia Vegetal – Abstract Book (N. Vieira, N. Saibo and M.M. Oliveira, Eds.), SPFV, 24-28 July, Lisbon, Portugal. pp. 393
Cristóvão MM, Goulao LF and Sánchez C (2013). Measuring transcript abundance of candidates from selected gene families: a first glimpse on the molecular basis of postharvest biocontrol in pears. Molecular Biology in Portugal and EMBL Conference: Conference Programme & Abstract Book, 18 July, Lisbon, Portugal. pp. 68.
Borém FM, Barbosa JN, Tosta MF, Leitão AE, Ribeiro DE, Cirillo MA, Ramalho JC, Sousa IN and Goulao LF (2012). Hardness of the coffee green bean relates to the environmental-driven sensorial quality of the beverage. ASIC (Association for Science and Information on Coffee) 2012: The 24th International Conference on Coffee Science – Programme and Abstracts, 11–16 November, San José, Costa Rica. pp. 115
Maçãs B, Coutinho JP, Almeida AS, Costa AR, Leitão AE, Leitão AL, Duarte MP, da Silva MMA, Fernando AL, Scotti-Campos P, Semedo J, Pais IP, Ramalho JC, Ribeiro AI, Goulão LF, Simões F, Matos J, Reboredo FH, Almeida M, Galinha C, Ribeiro V and Lidon FC (2012). Wheat crop in Portugal – biofortification prospective. COST ACTION FA 0905 Third Annual Workshop: Enhanced Nutritional Value of Plant-Derived Food or Feed program & Abstracts, 23-26 October, Almada, Portugal. pp. 50 (+ 4 supportive posters)
Sánchez C, Nunes A, Goulao LF, Coimbra MA and Barreiro G (2012). Insights into the mechanisms underlying the antagonistic action of A. pullulans against postharvest decay in pears. Resúmens of the POST LLEIDA 2012 - VII Iberian Postharvest Symposium. October 1-4, Lleida, Spain. pp. 105
Sousa PMF, Videira MAM, Bohn A, Goulão LF, Hood BL, Conrads TP and Melo AMP (2012). An integrated perspective of the Escherichia coli aerobic respiratory chain. European BioEnergetic Congress (EBEC). September 15-21, Freiburg, Germany. In: Biochimica et Biophysica Acta (BBA) – Bioenergetics 1817, Supplement. pp. S141
Ramalho JC, Leitão AE, Fortunato AS, Goulao LF, Melo AMP, Batista-Santos P, Santos P, Lopes E, Palos I, Lidon FC, Scotti-Campos P, Pais IP, Rodrigues AD, Jackson P, Lopes A, DaMatta FM and Ribeiro A (2012). Mecanismos de aclimatação a baixas temperaturas positivas em cafeeiro: Contribuição para a Investigação Agrícola para o Desenvolvimento no Contexto das Alterações Ambientais. IICT International Conference, Science in the Tropics: glimpsing the past, projecting the future, January 5-7, Lisboa, Portugal. pp. 56
Silva MJ, Ferreira-Pinto MM, Fortunato A, Goulão L, Leitão AE, Leitão C, Santos MR, Ramalho JC and Ribeiro A (2012). Avaliação e caracterização de genótipos de purgueira. IICT International Conference, Science in the Tropics: glimpsing the past, projecting the future, January 5-7, Lisboa, Portugal. pp. 86
Fernando AL, Simões F, Costa AR, Matos J, Reboredo FH, Leitão AE, Ramalho RJ, Almeida AS, Maçãs B, Coutinho J, Scotti-Campos P, Pais IP, Silva MMA, Duarte MP, Goulão LF, Ribeiro AI and Lidon FC (2011). I. Simultaneous biofortification of Zn and Fe in Triticum aestivum L. – A transgeneration survey. Abstracts of the First Annual Conference Mineral Improved Crop Production for Healthy Food and Feed – COST FA0905, 23-26 November, Venice, Italy. pp. 61 (+ 1 communication and 4 supporting posters)
Sousa PMF, Videira MAM, Silva STN, Carita J, Vaz F, Charro N, Penque D, Bohn A, Goulão LF, Hood BL, Conrads TP and Melo AMP (2011). Escherichia coli respiratory chain supercomplexes are present at the onset of aerobic growth. 2nd ITQB PhD Students’ Meeting: Book of Abstracts, October 26-28, Oeiras, Portugal. pp.54
Goulao LF, Fortunato AS, Ribeiro AI, Partelli FL and Ramalho JC (2011). A multiple analytical approach for
normalizing gene expression data of Coffea acclimation to changing environments. Livro de resumos da XIV Reunião Latino-Americana de Fisiologia Vegetal / XIII Congresso Brasileiro de Fisiologia Vegetal, 19-22 September, Búzios, RG, Brazil. pp.215
Fortunato AS, Goulao LF, Leitão AE, Rodrigues AD, Jackson P, Partelli FL, Ribeiro AI and Ramalho JC (2011). The role of the antioxidative system in Coffea sp. acclimation to simultaneous chilling and drought stress. VII Simpósio de Pesquisa dos Cafés do Brasil, 22-25 August, Araxá, MG, Brazil, 6 pp
Goulao LF, Fortunato AS, Jackson PA, Rodrigues A, Ribeiro AI and Ramalho JC (2010). Differential responses of the
antioxidant defense system to chilling and drought stresses in Coffea sp. genotypes - gene expression and antioxidant reinforcement. Congresso Nacional de Bioquímica, 15-17 December, Porto, Portugal. pp.305 Batista-Santos P, Partelli F, Campos PS, Pais IP, Fortunato AS, Goulão LF, Ribeiro AI and Ramalho JC (2010). The
effect of low temperature on metabolism of membrane lipids in Coffea sp. and associated gene expression. ASIC (Association for Science and Information on Coffee) 2010: The 23rd International Conference on Coffee Science - Programme & Abstracts, 3–8 October, Bali, Indonesia. pp.145
Fernandes JC, Lopes PC, Goulao LF and Amâncio S (2010). Exploring the Vitis cell wall transcriptome. XII Cell Wall Meeting Abstracts Book, 25-30 July, Porto, Portugal. pp.65
Goulao LF, Vatulescu AD, Ribeiro A, Ramalho JC, Jackson PA (2008). Developing a high trough-put method to
identify class III and ascorbate peroxidases of coffee differentially expressed under conditions of stress. 8th International Peroxidase Symposium, 20-24 August 2008, Tampere, Finland.
Goulao LF, Vieira-da-Silva S, Jackson PA (2007). Identification of hemicellulose- and pectin-modifying genes
expressed during Eucalyptus wood formation. XI Cell Wall Meeting, 12-17 August, Copenhagen, Denmark. Physiologia Plantarum 130(4) Abstract 230
Goulao LF, Vieira-da-Silva S, Jackson PA (2007). Cell wall modifications during xylogenesis in Eucalyptus globulus
involves divergent transcription of several members from candidate families. IUFRO Tree Biotechnology 2007, 3-8 June, Ponta Delgada - Azores, Portugal. pp.SVI.33
Alpalhão A, Neto C, Goulão L, Clemente J, Henriques J, Lourenço I, Oliveira CM (2006). Efeito do
1-metilciclopropeno em pêra ‘Rocha’ com diferentes estados de
maturação armazenadas em atmosfera normal. I Simpósio Nacional de Fruticultura: Programa e Resumos das Comunicações, 12-13 October 2006, Alcobaça, Portugal. pp.61
Goulao LF, Vatulescu A, Jackson PA (2006). Two peroxidases from Eucalyptus wood with role in cellular growth.
Peroxidase 2006: Abstracts & Programme, 6-9 July 2006, Aveiro, Portugal. pp.46
Neto C, Goulão L, Clemente J, Gomes MH, Duarte P, Almeida D and Oliveira C (2005). Efeito do 1-metilciclopropeno em maçãs e pêras armazenadas em atmosfera normal. V Congresso Ibérico de Ciências Hortícolas/IV Congresso Iberoamericano de Ciências Hortícolas, May 2005, Porto, Portugal. pp.382-389
Goulão L and Oliveira CM (2002). Cloning and characterization of a cDNA clone encoding an expansin isoform
with expression in ripening apples. Programa e Resumos do XIII Congresso Nacional de Bioquímica, 5-7 December 2002, Lisboa, Portugal. pp.57
Goulão L and Oliveira, CM (2002). Evidence of the presence of several isoforms of endo-beta-1,4-glucanases,
xyloglucan endotransglycosylase and expansins in over-ripe apples. 13th Congress of the Federation of European Societies of Plant Physiology: Book of Abstracts, 2-6 September 2002, Hersonissos - Crete, Greece. pp.338
Monte-Corvo L, Goulão L and Oliveira C (2000). Comparison of molecular markers (RAPD, AFLP, ISSR and SSR) on pear (Pyrus communis L.). VIII International Symposium on Pear Growing (ISHS), 4-9 September 2000, Ferrara-Bologna, Italy. pp.47-48
Goulão L, Farinha N, Sousa M and Martins J (1999). Caracterização morfológica de linhas de Trifolium subterraneum L. com base em análise multivariada. Programa e Resumos da XX Reunião de Primavera da
Sociedade Portuguesa de Pastagens e Forragens - Pastagens e Forragens em Pecuária Extensiva, 13-16 April 1999, Elvas, Portugal. pp.28
Goulão L, Cabrita L, Oliveira C and Leitão J (1998). Characterisation of apple (Malus x domestica Borkh.) cultivars
by AFLP markers. Programa e Resumos do XI Congresso Nacional de Bioquímica, 28-30 November, Tomar, Portugal. pp.138
Monte-Corvo L, Mota M, Goulão L, Silva D and Oliveira C (1998). Taxonomic use of RAPD markers in Pyrus. Abstracts of the XXV International Horticultural Congress (IHC), 2-7 August 1998, Brussels, Belgium. pp.445
O
THER
P
UBLICATIONS
:
DIVULGATION PAPERS:
Domingos S, Raposo AC, Ramalho JC, Oliveira CM and Goulao LF (2013). Efeito do ensombramento no vingamento e qualidade final em uva de mesa: Avaliação como método alternativo de monda de bagos. Revista da
APH - Associação Portuguesa de Horticultura 111: 23-27.
Domingos S, Oliveira CM and Goulão LF (2013). “Uva de mesa apirénica: técnicas de monda de flores e bagos -
Documento Técnico”. Offsetlis, Lda, Leiria, Portugal. 15 pp.
Rodrigues AD, Partelli FL, Ramalho JC, Palos IM, Fortunato AS and Goulao LF (2011). Uso do sistema antioxidativo como marcador de tolerância do cafeeiro arabica (Coffea arabica cv. Icatu) às baixas temperaturas positivas e secura. 37º Congresso Brasileiro de Pesquisas Cafeeiras - Mais tecnologia, mais café (Ed.: JB Matielo), Embrapa Café, November 1-4, Poços de Caldas, MG, Brazil. pp.289-290 (CD; ISSN 2176-3429) Oliveira C, Goulão L, Monte-Corvo L, Mota M, Nolasco G, Leitão J, Silva D and Sousa R (2000). Caracterização
biomolecular de cultivares portuguesas de macieira e pereira: Defender o património genético.
Investigação Agrária 1: 50-52.
Goulão L, Hilário L, Fortunato M and Nunes P (1998). A importância dos bancos de germoplasma na manutenção
da biodiversidade. Agros 81: 16-20.
GENBANK (NCBI) SUBMISSIONS: 69 SEQUENCES (Corenucleotide):
COFFEA ABIOTIC STRESS-RESPONSIVE PEROXIDASES:
JQ013432- JQ013441
EUCALYPTUS CELL WALL-MODIFYING ENZYMES INVOLVED IN WOOD FORMATION:
DQ100328-DQ100342, DQ376126-DQ376138, DQ645935-DQ645943
APPLE RIPENING-RELATED CELL WALL-MODIFYING ENZYMES:
AF527800, AY144593, AY144594, AY309436, AY350734, AY376878, AY530907
TRANSCRIPTS DIFFERENTIALLY EXPRESSED DURING APPLE RIPENING:
I
NVOLVEMENT IN
S
CIENCE &
S
OCIETY
A
CTIVITIES
:
SINCE 2012 – International "Fascination of Plants Day” - European Plant Science Organisation (EPSO) (2013: Participation in the “Mozambique Initiative – Our Plants, Our Life” and organization of the “Agri4Safe/IICT Initiative – Other beings in the plants”; 2012: Co-organization of the “Oeiras Initiative – Tropical Plants Tour”)
SINCE 2009 – European Researchers’ Night (2013: Organization of the activity: “Butterflies” in Foods; 2012: Organization of the activity: Biofuels – alternatives at high speed; 2011: Co-organization of a “Coffee-Science” Debate: Tropical Synergies and a Peddy-Paper: Lost and Found in the Tropical Forest (promotional event); 2010: Co-organization of a Peddy-Paper: Playing with Science; 2009: “Coffee-Science” debate - talk: Molecular Agriculture: a look at the genes to improve the production)
2013 – “Science Occupation in the Holydays” - “Ciência Viva” program (1-week lab course to 6 high-school students – How do we measure the expression of a gene?)
2013 –High school students (‘São João de Brito’ school) visit to IICT (Talk: “Research with Molecular Biology – Globalization of nucleic acids”)
2009, 2011 and 2012 –
“National Science & Technology Week” - “Ciência Viva” program (2012: “A Class in the
Palace” initiative - lecture: Food security for the tropics: our “fruitful” work; 2011: visit to high schools: “Chatting with scientists: another way of learning”; 2009: Short lecture: “Molecular Detectives”)
2011 – Participation in the event “Coffee – from the plant to the cup” (Lecture: Coffee, the dark beverage of white dreams), Casino Figueira da Foz
2005 and 2008 – Participation in the “ITQB’s Open Day” – Plant Division Stands
M
EDIA
A
PPEARANCES:
2014: Newspaper article: Público – supplement: Fugas; section: Gardening: “Stevia, a erva doce sem açucar” (published 29th March; page 23)
2014: Newspaper article: Público – supplement: Fugas; section: Gardening: “Sabia que pode produzir uvas sem grainha num vaso?” published 8th February; page 31)
2012: Pre-recorded interview with Ana Paula Gomes: RDP ÁFRICA Radio - Program Cientificamente: “Science as a tool to achieve the Millennium Development Goals” (broadcasted 17th November)
2012: Pre-recorded interview with Edgar Canelas: RDP ANTENA 1 Radio – Program Os Dias do Futuro: "The Fascination of Tropical Plants" (broadcasted 12nd May)
