Dissertação de Candidatura ao grau de Mestre em Oncologia submetida ao Instituto de
Ciências Biomédicas de Abel Salazar da Universidade do Porto.
Orientador – Doutor António José Braga Osório Gomes Salgado
Categoria – Investigador auxiliar
Afiliação – Instituto de Investigação em Ciências da Vida e da Saúde (ICVS), Escola de Ciências da Saúde, Universidade do Minho
Co-orientador – Professor Doutor Carlos Lopes
Categoria – Professor Catedrático
Afiliação – Instituto de Ciências Biomédicas
Abel Salazar da Universidade do Porto
iii
in vitro
in vivo
in vivo
v
in vivo
vi
in vitro
in vivo
in vivo
vii
viii
1
2
Minimal Essencial Medium Alpha Medium
Brain derived neutrophic factor Basic fibroblast growth factor
Epidermal growth factor
Fetal Bovine Serum Fetal Calf Serum
Glial cell derived neurotrophic factor Glial fibrillary acidic protein
Hepatocyte growth factor
Insulin growth factor
3
Nerve growth factor
Stem cell factor
Vascular endothelial growth factor
4
et al.
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et al.
et al.
et al.
et al.
et al.
5
et al.
et al.
Morris Water Maze
6
7
11
et al.
12
13
β α
14
standard
et al.
15
16
et al.
et al.
in vitro
17
Musashi-1 Nestin Sox
18
nerve growth factor brain-derived neurotrophic factor
ciliary neurotrophic factor vascular endothelial growth factor fibroblast growth factor 2
19
et al.
α
in vitro 20
21
in vitro in vivo
22
et al
Insulin growth factor Epidermal growth factor
in vivo
et al
23
in vitro et al.
et al.
et al.
24
et al.
et al.
in vitro
25
et al.
et al.
in vitro
et al in vitro,
26
27
et al.
28
platelet-derived growth factor
29
33
et al.
loop
Alpha MEM
37
Morris Water Maze
38
39
rabbit anti-rat glial fibrillary acid protein
mouse anti-rat microtubule associated protein 2
Human nuclear antigen Nestin
5bromo-2- deoxyuridine
40
goat anti-mouse immunoglobulin goat anti-rabbit
41
45
46
47
in vivo
et al
et al
In vitro 48
in vitro
49
et al
in vitro In vivo
50
in vivo
in vitro
51
52
et al
53
54
et al
Morris Water Maze
55
56
et al
57
58
in vivo
in vitro
61
Academic Press
Neuron Glia Biology
Physiological Reviews
J Neuroimmune Pharmacol.
J.Comp. Neurol
Neuroscience and Biobehavioral Reviews
Anat. Rec.
Journal of Neuroscience Research
Psychology and Aging
Nature
Progress in Neurobiology
65
The Journal of Neuroscience
Annu. Rev. Neuroscience
Neuropharmacology
Neuroscience Research
HIPPOCAMPUS
J. Pharmacol. Exp. Ther.
J. Neurosci.
Annu.
Rev. Cell Dev. Biol.
Annu. Rev. Psychol.
J.
Neurosci.
BMB reports
66
Brain Res. Dev. Brain Res.
Genome Biology
Journal of Neuroscience Research.
J. Neural. Transm
Progress in Neurobiology
Cell and Tissue Research
67
Neurosci. Biobehav.
Rev.
Ortop Traumatol Rehabil
J. Clin. Invest
Cell
Cell.
Annu. Rev. Med.
Stem Cells and Development
Journal of Neuroscience Research
Annu. Rev.
Cell. Dev. Biol.,
Genes & Development
68
Circulation
Science
Journal of Cell Science
Stem cells
Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America
in Satellite Symposium on Neural Stem Cells and Brain Repair
Trends Neurosci.
Nat Neurosci.
69
Human Molecular Genetics
Drug. Discov.
Today.
European Journal of Dentistry
Proc. Jpn. Acad.
BMC Medicine
Phil. Trans. R. Soc. B
et al.
Dev. Neurosci.
et al.,
Cell
et al Development
Cell Research
Nature Medicine
Journal of Cellular Biochemistry
Ann Acad Med Singapore.
70
Proc. Natl. Acad. Sci. U.S.A.
Nat. Neurosci.
Nature.
Physiology & Behavior
Experimental Neurology
Developmental Brain Research
Journal of Neuroscience
Experimental Neurology
Neuroscience Letters
Aging Cell
71
Crit Rev Oncol Hematol
Journal of Neuroscience Research
Medical Hypotheses
et al
Transplantation
, et al.
Cytotherapy,
, et al.
Cytotherapy
Journal of Cellular Biochemistry
, et al.
Science
, et al.
Blood
, et al.
Haematologica 72
, et al.
Tissue Eng
, et al.
Mol Biol Cell
J Bone Miner Res
Proc Natl Acad Sci U S A
, et al.
Transplantation
Br J Haematol
Stem Cells Review
, et al.
Stem Cells , et al.
Stem Cells
Stem
Stem Cells
73
Cytokine Growth Factor Rev
Journal of Pathology
Trends Mol Med , et al.
Nat Med
, et al.
Transplantation
, et al.
Proc Natl Acad Sci U S A
et al.
Cytotherapy
Archives of Medical Research
Neurochemical Research
74
Proceedings of the National Academy of Sciences of the United States of America
Bone Marrow Transplantation
Experimental Neurology
Journal of Neuroscience Research
Stem cells
Neuropathology
ANZ J Surg
et al
in vitro Proteome Science
Stem cells
Stem cells
75
PLOS one
Experimental Neurology
Stem cells
Stem cells
Cytotherapy
Stem cells
Stem Cells and Development
Biochimica et Biophysica Acta
et al.
Cancer Cell.
76
Oncogene
Stem Cells and Development
Development
Neoplasma
Neurol Clin.
Annu. Rev. Cell Dev. Biol.
Stem Cells and Clonig: Advances and Applications
Ann. Neurol.
N Engl J Med
et al
The Journal of Biological Chemistry
Biotechnol.J.
77
Academic Press, San Diego, USA
Journal of Neuroscience Pain
et al
Experimental and Molecular Medicine
Critical Review in Immunology
et al Stem Cells
J Mol Med.
78
