Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences GE Scientific and Research Institute of Biomedical Chemistry named after. Academician of the Russian Academy of Medical Sciences, Academician of the Russian Academy of Agricultural Sciences, Director of the Biomedical Technology Center,. Academician of the Russian Academy of Medical Sciences, Director of the Institute of Biomedical Chemistry of the RAMS, vice-.
Academician of the Russian Academy of Medical Sciences, Academician of the Russian Academy of Agricultural Sciences, Advisor to the Russian Academy of Sciences. Academician of the Russian Academy of Agricultural Sciences, Director of the All-Russia Research Institute of Agricultural Biotechnology RAAS. Corresponding member of the Russian Academy of Medical Sciences, deputy of the Duma RF, chairman of the platform "Doctor of the Future".
Academician of the Russian Academy of Sciences, Academician of the Russian Academy of Agricultural Sciences, Director of the Center. Academician of the Russian Academy of Medical Sciences, Director of the Nutrition Research Institute of the Presidium of the Academy of Medical Sciences.
Petrov
Beregovyh
Academician of the Russian Academy of Agricultural Sciences, Director of the All-Russia Research Institute of Milk Industry of the Russian Academy of Agricultural Sciences. Academician of the Russian Academy of Agricultural Sciences, Director of the All-Russia Research Institute of Agricultural Biotechnology of the Russian Academy of Agricultural Sciences. Academician of the Russian Academy of Sciences, Director of the Institute of Cytology and Genetics of the RAS Siberian Branch, Novosibirsk.
PhD (Medicine), First Deputy Director of the Institute of Immunology of the Ministry of Healthcare of the Russian Federation. Petrov Academician of the Russian Academy of Medical Sciences, Rector of Volgograd State Medical University. Polaykov Academician of the Russian Academy of Agricultural Sciences, Director of the Institute of Food Biotechnology RAAS.
Bach Institute of Biochemistry of the Russian Academy of Sciences, Chairman of the platform "BioTech 2030". Corresponding member of the Russian Academy of Sciences, Director of the All-Russia Research Center for Molecular Diagnostics and Therapy.
PhD, Professor, Deputy Director of the Central Research Institute of Geological Research for Base and Precious Metals. Academician of the Russian Academy of Sciences, Academician of the Russian Academy of Medical Sciences, Director of the Institute of Pharmacology of RAMS named after V.V. Corresponding Member of the National Academy of Sciences of Belarus, Member of the Presidium of the National Academy of Sciences of Belarus, Director of the Institute of Bioorganic Chemistry of NAS, Minsk, Belarus.
Vlasov
Afonchikova, Klimov
The aim of the study was to investigate the state of the proliferative processes on breast carcinoma cells, depending on protein Her-2/neu expression and Her-2 gene amplification. Proliferative activity of the explored tumor can be evaluated by percentage ratio of stained nuclei of carcinoma cells to unstained ones. Presence of the gene amplification has no influence on the change of proliferative activity of breast carcinoma.
Combined treatment of the HCT116 cells with bortezomib (10 nM) and taxol (1 nM) decreased effective doses (ED50) of DR5-B 5-10 times, while this parameter was not changed for wild-type TRAIL. Among the problems facing modern society, the state of the environment is one of the first places. Only in the European and British pharmacopoeias is the method of quantitative determination of Clostridium perfringens introduced, as one of the most pathogenic and common anaerobic contaminants.
As a result of elaboration, it was shown that any of the three fluorescent dyes (Hoechst 33342, DyeCycle Violet and Rhodamine 123) can be used for the analysis of "side population" on a flow cytometer equipped with blue and violet lasers. As a result of the analysis of biological material, it was found that the number of "side population" cells associated with cancer stem cells was in the cells of human colorectal adenocarcinoma and in the presence of verapamil it was reduced to.
Kim, Marine E. Gasparian
Lactobacilli dominate the normal vaginal microbiota and confer colonization resistance of the biotope due to their antagonistic activity against pathogenic and opportunistic microorganisms. The low value of the index (<50%) indicates a decrease in antagonistic activity and the number of lactobacilli in the vaginal flora and thus biotope colonization resistance. The mutagenesis technique was based on reverse PCR amplification of the entire expression plasmid.
This may be due to the release of the enzyme in the pores of the carriers, and the interaction of the aldehyde groups of the modified polymer with the protein globules. Most of the tissue engineering approaches increasingly used in modern medicine for the reconstruction of bone defects are based on the seeding of osteoprogenitor cells on a biodegradable scaffold. Of the tested materials (brushite-hydroxyapatite cement, hydroxyapatite cement, tricalcium phosphate prepared by gypsum conversion, tricalcium phosphate ceramic), a greater rate of cell survival and proliferation was observed in the case of cell culture in the presence of tricalcium. ceramic (Fig. 3).
In other studies, the possibility of efficient integration of the fusion protein into the magnetosome membrane by sonication in the presence of a nonionic detergent was demonstrated [3]. Spectroturbodimetry and photon correlation spectroscopy methods were used to characterize the aggregates in terms of particle diameter and degree of heterogeneity. The physicochemical characteristics of loaded liposomes were studied by dynamic light scattering and X-ray fluorescence analysis.
The aim of the research was to determine the cause of DNA damage and the mode of action of CL. We prepared and investigated two series of MBBT nanodispersions: by fixing the solution concentration (2% by weight of STB) and modifying the porphyrin content 5%, 10%, or by fixing the porphyrin concentration (2%) and varying the amount of added cholesterol hemisuccinate (CHS). Conjugation concentration dependences were studied and the optimal composition of the resulting immunosorbent was determined.
Determination of the number of DNA duplexes on the surface was performed by counting the number of gold nanoparticles in the areas of the microchip. The method is characterized by high sensitivity and specificity, as well as a high analytical signal/background ratio. L-carnitine has a quaternary ammonium structure in its structure, which is theoretically promising for use as part of polar cationic amphiphiles.
From the concentration dependence of the effect of the nanoparticles in the range of dilutions of the stock solution of nanoparticles, the toxicity threshold (the concentration corresponding to the first signs of toxic effects) and lethal concentrations were found. The study of rheological behavior of water solutions showed the change of flow type at the same component ratio.
Antonov Y.A
V. Khmelev
- N. Makarov
These methods make it possible to detect the functional changes at the cellular level with exact anatomical localization of the pathological focus. One of the characteristics of modern medicine is the expensive associated with high-tech treatments and increased control, insurance company for the quality of medical treatment. The most important issue is the preservation of the natural environment, which determines the quality of life and health.
Specific expression of the Tn antigen on the surface of malignant cells is the basis for the development of anti-cancer vaccine containing the Tn epitope. In addition, the clusters composed of two or three consecutive glycosylated serine/threonine (Ser/Thr) also contributed to the formation of the Tn epitope. To further investigate the nature and complexity of the Tn antigen, we generated a panel of anti-Tn mAbs.
IgM mAbs bound the terminal GalNAc residue of the Tn antigen regardless of the peptide context. A significant protective effect of the staphylococcal vaccine was found in Balb/c mice infected with a virulent strain of S.aureus. It was demonstrated that the antigenic sites of SP-platforms were masked by CaMV virions bound to the SP surface.
Digestion of the food from plants belonging to the legume family often causes some local or systemic allergic reactions. Key words: formalin emulsion vaccine (FEV), bovine necrobacteriosis, fuzobacsen (FBS), cattle finger and hoof diseases, F.necrophorum. Many of the regulatory proteins contain hairpin loop structures capable of being involved in the interaction with the receptor.
To mimic the homodimeric structure of NT, resulting substituted dipeptides were joined via hexamethylenediamine spacers. One of the secondary metabolites widespread in the plant kingdom is the alkaloid berberine. In Russia, the main raw material for the production of berberine is the bark of the roots of barberry and common barberry Amur.
Today, one of the most popular clinical trials is cholesterol testing due to the high prevalence of atherosclerotic heart disease. The aim of the current research is to prepare a domestic cholesterol oxidase production technology.
Krementsova A.V., Burlakova E.B
Доза фенозана 10-14 моль/кг также замедляла течение лейкемического процесса: антиоксидант не только увеличивал среднюю продолжительность жизни более чем на сорок дней и снижал заболеваемость лейкемией на 6%, но и увеличивал максимальную продолжительность жизни. продолжительность жизни 69 дней.