Antisense oligonucleotides
Antisense Oligonucleotides Modulating Activation of a Nonsense-Mediated RNA Decay Switch Exon in the ATM Gene
... identify antisense oligonucleotides that modulate NSE inclusion in mature transcripts by systematically targeting the entire ...distant antisense Alu and NSE activation upon elimination of a long ...
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Modulation of p53 expression using antisense oligonucleotides complementary to the 5'-terminal region of p53 mRNA in vitro and in the living cells.
... In the presence of oligomer no. 7 complementary to the apical loop and partially to the 3 9 side of the U180-A218 hairpin the translation efficiency from AUG1 decreased only slightly even at 2 mM oligomer concentration. ...
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Dynamics of co-transcriptional pre-mRNA folding influences the induction of dystrophin exon skipping by antisense oligonucleotides.
... Antisense oligonucleotides (AONs) mediated exon skipping offers potential therapy for Duchenne muscular dystrophy. However, the identification of effective AON target sites remains unsatisfactory for lack ...
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RNA Interference-Guided Targeting of Hepatitis C Virus Replication with Antisense Locked Nucleic Acid-Based Oligonucleotides Containing 8-oxo-dG Modifications.
... as antisense technology. Antisense oligonucleotides (ASOs) are the major class of antisense agents used for sequence-specific RNA knockdown [1], and they can also be used to modulate RNA ...
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Antisense Oligonucleotide technologies for neuronal cell regeneration after spinal injury
... stranded antisense oligonucleotides (AON) associate with RNAse H to promote degradation of target mRNA, with the possibility of a recycling mechanism and long-lasting ...effects. Oligonucleotides are ...
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Papel da PI3K8 no controle da contratilidade vascular no Diabetes Mellitus I: estudo funcional e eletrofisiológico
... Diabetes is associated to damage in several organs and systems including the vascular system. Vascular diseases are the main cause of mortality and morbidity in diabetes patients. However, the mechanisms that determine ...
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Somatic gene therapy for hypertension
... the antisense direction expresses antisense mRNA to compete with sense mRNA, ap- pears to be effective for reducing high blood pressure in models of ...Alternatively, antisense ...
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A Cytoplasmic New Catalytic Subunit of Calcineurin in Trypanosoma cruzi and Its Molecular and Functional Characterization
... with antisense oligonucleotides was not due to diminished parasite viability, we performed an additional assay that consisted of determining the parasite’s capacity to migrate through a polycarbonate ...
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IL-1ß and BMPs - Interactive players of cartilage matrix degradation and regeneration
... BMP-7 antisense oligonucleotides reduced aggrecan gene expression in adult cartilage and lead to proteoglycan depletion of the cartilage matrix (Soeder et ...
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Quím. Nova vol.34 número9
... phosphorothioate oligonucleotides association efficiency was found, for a given for- ...egg-lecithin), oligonucleotides were able to associate with ...
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To nick or not to nick: comparison of I-SceI single- and double-strand break-induced recombination in yeast and human cells.
... contrary, the K223I nickase stimulated recombination between direct repeats equally well in wild-type and rad51D cells and promoted gene correction by oligonucleotides in a Rad51- dependent manner (Figure 2B and ...
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Delivery of miRNA-targeted oligonucleotides in the rat striatum by magnetofection with Neuromag®
... delivery oligonucleotides in the brain is still an unmet ...delivering oligonucleotides into the central nervous system ...active oligonucleotides in vivo ...
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Poly-thymidine oligonucleotides mediate activation of murine glial cells primarily through TLR7, not TLR8.
... The functional role of murine TLR8 in the inflammatory response of the central nervous system (CNS) remains unclear. Murine TLR8 does not appear to respond to human TLR7/8 agonists, due to a five amino acid deletion in ...
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Exonization of an Intronic LINE-1 Element Causing Becker Muscular Dystrophy as a Novel Mutational Mechanism in Dystrophin Gene
... forward oligonucleotides were designed to encompass these five regions of interest and a single reverse oligonucleotide annealing to the known inserted LINE-1 sequence (detected by cDNA ...
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J. bras. pneumol. vol.32 número3 en a10v32n3
... with oligonucleotides -actin 5' (5' - AGC GGG AAA TCG TGC GTG - 3') and -actin 3' (5' - CAG GGT ACA TGG TGG TGC - 3'), which amplify a 304-bp segment of human β-actin ...
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A spontaneous dominant-negative mutation within a 35S::AtMYB90 transgene inhibits flower pigment production in tobacco.
... Finally, transgenic tobacco plants expressing a double-stranded hairpin construct targeting the entire NtAN2 coding region for RNAi (ihpNtAN2, a 35S::antisense-intron-sense hairpin withi[r] ...
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Membrane-enriched proteome changes and prion protein expression during neural differentiation and in neuroblastoma cells
... Oligonucleotides used for qPCR of mouse specific genes & Protein spots differentially abundant during neural differentiation of ES cells (set #1, fold change ± 1.5) and between neuro[r] ...
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BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
... Key words: Molecular biology, molecular imaging, PET, nucleosides, nucleotides, nucleic acids, oligonucleotides, antisense, aptamer, spiegelmer.. Author for correspondence INTRODUCTIO[r] ...
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Antisense mRNA for NPY-Y1 receptor in the medial preoptic area increases prolactin secretion
... Intracerebroventricular microinjection of NPY at the doses of 235 and 470 pmol (11) or intracardiac administration of anti-NPY serum (20) induced an increase in plasma LH in ovariectomized rats. NPY has been shown to ...
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Correction of a Splicing Mutation Affecting an Unverricht-Lundborg Disease Patient by Antisense Therapy
... an antisense oligonucleotide therapeutic strategy could correct the defect in patient ...(LNA) antisense oligonucleotide was designed to block a cryptic 5 0 ss in intron ...translating antisense ...
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