Conditioned medium
Human embryonic mesenchymal stem cell-derived conditioned medium rescues kidney function in rats with established chronic kidney disease.
... The potential of CM to stimulate endothelial cell migration was assessed by in vitro scratch wound assay. A mechanical scratch was created with a pipet tip in a confluent monolayer of HMECs. After washing with PBS, 200 m ...
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Effect of conditioned medium of mesenchymal stem cells on the in vitro maturation and subsequent development of mouse oocyte
... Figure 3. Follicular growth in ovarian organ cultured on soft agar. A, At day 2, the preantral follicles began to initiate growth, and protruded from the surface of cultured organ due to increase in follicle diameter ...
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Conditioned medium from activated spleen cells supports the survival of rat retinal cells in vitro
... that conditioned medium from spleen cells activated with concanavalin A increased neuritogenesis and survival of retinal cells, as measured by biochemical and morphological crite- ...that conditioned ...
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Induction of chagasic-like arrhythmias in the isolated beating hearts of healthy rats perfused with Trypanosoma cruzi-conditioned medium
... Vero-infected medium; therefore, we suggest that pro- teins present in the conditioned medium have the ability to enhance arrhythmias, probably by increasing the intra- cellular calcium ...
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Characterising the mechanism of airway smooth muscle β2 adrenoceptor desensitization by rhinovirus infected bronchial epithelial cells.
... In order to identify whether there was a difference in the peptide profiles between the control and RV conditioned medium that were in the ,3 kDa fraction, mass spectrometry (MS) was carried out as ...
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Establishing a High Titer Transient Gene Expression Process in Conditioned Media for CHO-DG44 Cells
... a medium exchange before ...a medium exchange (i.e in conditioned medium), the yields obtained are very low (~5 ...in conditioned medium and utilizing sodium butyrate as a ...
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Activity deprivation induces neuronal cell death: mediation by tissue-type plasminogen activator.
... TTX-conditioned medium (containing TTX) was as potent as freshly-added TTX in eliciting cell death, and that the conditioned medium of cells in their recovery period is not toxic at all to ...
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Bone Marrow-Derived Mesenchymal Stem Cells Repaired but Did Not Prevent Gentamicin-Induced Acute Kidney Injury through Paracrine Effects in Rats
... DMEM medium (Sigma, ...The medium was changed regularly every 3 ...the conditioned medium-treated groups, the BMSCs were cultured with DMEM without SBF for 24 ...
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Arq. Bras. Oftalmol. vol.76 número2
... stimulation. Conditioned medium from SHR-stimulated MSCs and control MSCs were collected for evaluation their effect on retinal ...(p<0.01). Conditioned medium from mesenchymal stem cells ...
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Jejunal proteins secreted by db/db mice or insulin-resistant humans impair the insulin signaling and determine insulin resistance.
... and conditioned medium contain factor/s, which are likely in a larger amount in the insulin resistant than in insulin sensitive subjects, that induce insulin ...
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Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and enhance wound healing.
... BM-MSC-conditioned medium by antibody-based protein array and ELISA indicated that BM- MSCs secreted distinctively different cytokines and chemokines, such as greater amounts of VEGF-a, IGF-1, EGF, ...
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Inhibition of Wnt/β-catenin signaling by a soluble collagen-derived frizzled domain interacting with Wnt3a and the receptors frizzled 1 and 8.
... Figure S3 FZC18 reduces basal level and Wnt3a-induced b- catenin stabilization and cyclin D1 expression. (A) b-catenin assay stabilization assay using anti-b-catenin, anti-non-phosphorylated b-catenin and anti-GAPDH ...
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Preconditioning via angiotensin type 2 receptor activation improves therapeutic efficacy of bone marrow mononuclear cells for cardiac repair.
... of conditioned medium collected from AT2R stimulated BMMNCs, which was abolished when incubated HUVEC with DMEM or conditioned medium from non- preconditioned BMMNCs or BMMNCs treated with ...
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Effects of androgen receptor and androgen on gene expression in prostate stromal fibroblasts and paracrine signaling to prostate cancer cells.
... of conditioned medium from WPMY-AR or WPMY-Vec cells, both treated with DHT, that was then supplemented into fresh medium that also contained ...WPMY-AR conditioned medium significantly ...
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Control of human endometrial stromal cell motility by PDGF-BB, HB-EGF and trophoblast-secreted factors.
... Non-directed migration was determined using the Oris TM Cell Migration Assay (AMS Biotechnology, Abingdon, U.K.). Princi- pally, a central silicon stopper is inserted into each well of a black 96-well culture plate with ...
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The secretome of alginate-encapsulated limbal epithelial stem cells modulates corneal epithelial cell proliferation.
... Protein adsorption to alginate hydrogels was previously reported [23]. Therefore, diluting the CM ALG with fresh CnT20 partially replenishes some of the growth factors explaining the moderate increase in proliferation. ...
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PABLO HERTHEL DE CARVALHO TRANSPLANTE ALOGÊNICO DE CÉLULAS-TRONCO MESENQUIMAIS INDUZ ALTERAÇÃO DO PERFIL DE CITOCINAS PRÓ E ANTINFLAMATÓRIAS E DE FATORES NEUROTRÓFICOS NO
... Conditioned medium from bone marrow- derived mesenchymal stem cells improves recovery after spinal cord injury in rats: an original strategy to avoid cell transplantation.. Mesenchy[r] ...
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INTRASPECIFIC AND INTERSPECIFIC PRE-ADULT COMPETITION ON THE NEOTROPICAL REGION COLONIZER ZAPRIONUS
... banana-agar medium, replicated 10 times: ...were conditioned each one with 30 larvae one-day-old: vial 2 was conditioned with intraspecific residues (Vzp, Vst or Vsm), vial 3 with interspecific ...
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Macrophage-associated mesenchymal stem cells assume an activated, migratory, pro-inflammatory phenotype with increased IL-6 and CXCL10 secretion.
... triplicate. Conditioned medium samples displayed in Figure 2 were analyzed by the Cytokine Core Laboratory at the University of Maryland (Baltimore, ...
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High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress.
... For conditioned medium, cells were rinsed once with serum free medium and incubated with serum free medium (complete medium with different conditions of glucose but no serum) for two ...
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