Conventional PCR
Comparison of real-time PCR and conventional PCR for detection of
... to conventional PCR include speed, reproducibility and quantitative ...replace conventional PCR in diagnostic ...real-time PCR in highly complex diagnoses (reference services) in ...
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Conventional PCR assays for the detection of pathogenic <i>Vibrio</i> spp. in shrimp aquaculture in the Philippines
... Conventional PCR as a diagnostic tool in ...following PCR may be sufficient to indicate whether the sample is infected by a certain ...the PCR products are employed (McBeath et al ...
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Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient
... Before DNA extraction from spermatozoa, there were two washes with PBS by centrifugation at 900x g for 5min to remove as much residues of the density gradient as possible. DNA extraction from sperm samples was performed ...
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DETECTION OF TRANSGENIC EVENTS IN MAIZE USING IMMUNOCHROMATOGRAPHIC STRIP TEST AND CONVENTIONAL PCR
... the PCR test (CUNHA et ...2002). Conventional PCR is a widely disseminated technique in various areas of science, providing precise and reliable analysis for detection of transgenic plants; however, ...
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A conventional PCR for differentiating common taeniid species of dogs based on in silico microsatellite analysis
... Canine taeniids are among the major tapeworms with remarkable medical and economic significance. Reliable diagnosis and differentiation of dog taeniids using simple and sensitive tools are of paramount importance for ...
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Conventional PCR for detection of Corynespora cassiicola in soybean seeds
... The pair of primers GA4-F/GA4-R detected C. cassiicola in soybean seeds (Figure 3) with the inoculum potential P1 with incidence levels ranging from 10% to 100%; in P2, as of 1% to 100%; in P3, the minimum artificial ...
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Use of conventional PCR and smear microscopy to diagnose pulmonary tuberculosis in the Amazonian rainforest area
... with conventional polymerase chain reaction (ZN/PCR) to amplify IS6110 region DNA extracted from ZN slides was ...ZN, PCR, and culture performed in the sputum samples from these patients were ...
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Expression of phosphoinositide-specific phospholipase C isoforms in native endothelial cells.
... A conventional PCR approach was initially used to elucidate the mRNA expression pattern of PLC isoforms in 3 distinct murine vascular beds: mesenteric (MA), pulmonary (PA) and middle cerebral arteries ...
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Rev. Soc. Bras. Med. Trop. vol.50 número4
... different PCR approaches in terms of sensitivity, cost-effectiveness, and time ...requirements. Conventional PCR showed great sensitivity and did not detect only the last dilution (1 PFU ...
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Rev. Assoc. Med. Bras. vol.52 número4
... the conventional polymerase chain reaction analysis of the maternal plasma can be used for the fetal RHD genotyping, even in a miscellaneous ethnic popu- ...using conventional PCR and is the first ...
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Preclinical detection of porcine circovirus type 2 infection using an ultrasensitive nanoparticle DNA probe-based PCR assay.
... based PCR assay could detect 2 copies of PCV2 recombinant plasmid per reaction and 10 viral copies of PCV2 in per ml serum, whereas conventional PCR just could detect over 1610 3 copies of PCV2 ...
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Detection of Sclerotinia sclerotiorum in soybean seeds by conventional and quantitative PCR techniques
... study conventional and quantitative PCR techniques were used to assess their viability to detect ...The conventional PCR was able to detect ...
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Real-time PCR for detection of Brucella ovis and Histophilus somni in ovine urine and semen
... Infectious ovine epididymitis affects rams worldwide, and it is considered to be one of the most important infectious diseases (Poester et al., 2013). Brucella ovis is one of the most important infectious agents ...
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PCR TECHNOLOGY CONVENCIONAL PCR
... through PCR technology. Unlike conventional methods, samples can be tested directly through PCR and isolated without the need for ...methods. PCR can be performed routinely and does not ...
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DEVELOPMENT OF CONVENTIONAL AND REAL-TIME MULTIPLEX PCR ASSAYS FOR THE DETECTION OF NOSOCOMIAL PATHOGENS Deepa Anbazhagan
... Multiplex PCR has been used successfully for rapid detection, with high specificity and sensitivity of various pathogenic bacteria from environmental waters (3) and various food products (4, 29, 13, 21, 25, 11, ...
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Genetic variants associated with ectopic calcifications
... Analysis of sequence conservation among six vertebrates of variants identified in candidate genes possibly associated with DISH/CC phenotype... Conventional PCR amplification fo[r] ...
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Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR
... real-time PCR alone or con- ventional PCR, although this difference was more notable when comparing nested-PCR or real-time PCR with con- ventional ...as conventional PCR, and ...
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Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR
... as PCR can provide fast and reliable results while significantly decreasing the time of ...with conventional PCR followed by real-time PCR, was developed to detect Mycobacterium tuberculosis ...
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MATERIALS AND METHODS Bacterial strains: The Burkholderia strains used in this study were
... lpxO conventional PCR & sequencing lpxO realtime PCR: When we used conventional PCR primers targeting a 900bp ...corresponding PCR product in 64 of 72 ...lpxO PCR ...
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Brazilian ground beef authentication by multiplex polymerase chain reaction
... multiplex PCR protocols reported in the literature are based on the hypothesis that each forward primer has a corresponding reverse primer (BAI et ...multiplex PCR, conventional PCR using the ...
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