Dot-blot
Immuno-dot blot para detecção do vírus da dengue em Aedes aegypti e em Aedes albopictus
... Western blot e dot blot. No dot blot, um antígeno da partícula viral, que já tenha sido isolado e purificado a partir do vírus ou do hospedeiro contaminado, é primeiramente aplicado na ...
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RT-PCR AND DOT BLOT HYBRIDIZATION METHODS FOR A UNIVERSAL DETECTION OF TOSPOVIRUSES
... Transcriptase reverse - polymerase chain reaction (RT- PCR) and dot blot hybridization with digoxigenin-labeled probes were applied for the universal detection of Tospovirus species. The virus species ...
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Detecção do Grapevine virus A e Grapevine virus B por hibridização dot-blot com sondas moleculares não radioativas
... As sondas produzidas apresentaram especificidade e sensibilidade na detecção do GVA e do GVB, podendo ser utilizadas como alternativa aos testes sorológicos para identificação desses vírus e para as triagens iniciais de ...
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Seleção de colônias de Clostridium perfringens tipo D produtoras de toxina épsilon por "dot blot"
... “dot blot” (Figura ...“dot blot” não foi sensível o suficiente para detectar pequenas quantidades da toxina épsilon produzida pelos clones considerados negativos, de acordo com a intensidade ...
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Clinical and molecular characterization of feline mammary carcinomas overexpressing HER2 proto-oncogene (FMC-HER2+) : new strategies for effective diagnostic and cancer therapy
... After testing for normality, the Wilcoxon test was used to compare the sHER2 levels between cats with mammary carcinoma and healthy ones, and also to compare sHER2 levels and tissue HER2 status among cancer group cats. ...
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Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
... reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 ...
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Visceral Leishmaniosis Caused by Leishmania (L.) mexicana in a Mexican Patient with Human Immunodeficiency Virus Infection
... A 36 year old male was admitted in December 1997 to hospital with afternoon fever, malaise and hepatosplenomegaly. He also had a dry cough, dyspnoea and anaemia. Pneumonia caused by Pneumocystis carinii and human ...
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Arq. NeuroPsiquiatr. vol.69 número3
... and dot blot in 13 serum and 13 CSF samples from patients with neurocysticercosis, 3 CSF samples from patients with other neurological disorders, 3 serum samples from healthy individuals and 44 serum ...
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Biosynthesis, Isolation and Magnetization of gellan spheres for biorecognition of therapeutic His-tag proteins
... - DOT-BLOT analysis of the recovered supernatant from 10 mL of spheres with nickel as a cross- linker and from magnetic spheres, through the batch ...
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Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins
... In dot blot assay 100 ng (1 µL) of each protein were spotted onto nitrocellulose membrane (Whatman – GE Healthcare, Glattbrugg, Switzerland) and dried for 1 h at room ...2010). Dot blots of 10 ...
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Identification of a conserved B-cell epitope on duck hepatitis A type 1 virus VP1 protein.
... To verify that the identified motif represented the epitope recognized by mAb 2D10 and duck anti-DHAV-1 sera, peptides representing the motif LPAPTS were synthesized. Dot blot analy- sis showed that the ...
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Padronização do Elisa indireto e Western Blot para diagnóstico da artrite-encefalite caprina.
... PINHEIRO, R.R. Vírus de Artrite Encefalite Caprina: Desenvolvimento padronização de ensaios imunoenzimáticos (Elisa e Dot-Blot) e estudo epidemiológico no Estado do Ceará. 2001. 115f. Tese (Doutorado em ...
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Lipopolysaccharide Specific Immunochromatography Based Lateral Flow Assay for Serogroup Specific Diagnosis of Leptospirosis in India.
... (ELISA), dot blot assay and rapid immunochromatography based lateral flow assay ...and dot blot assay using various lep- tospiral LPS was >90% for homologous ...
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Multiple cytokine expression profiles reveal immune-based differences in occult hepatitis B genotype H-infected Mexican Nahua patients
... Increased TGF-β levels in OHB-infected patients supports a differential anti-inflammatory process - Our data suggests a difference between the pro-inflamma- tory profiles of the OHB-infected patients compared to the ...
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Caracterização de anticorpos monoclonais contra rotavírus bovino e suas aplicações como ferramenta de diagnóstico.
... proteínas virais pela metodologia de western-blot (Fig. 2). O AcM 4F3 reagiu somente em condições nativas, obedecendo provavelmente ao reconhecimento de um epitopo conformacional. Quando esse anticorpo foi usado ...
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IMPORTÂNCIA DA AVALIAÇÃO HEMATOLÓGICA E SOROLÓGICA (DOTBLOT ELISA) NO DIAGNÓSTICO DE ERLIQUIOSE EM CÃESJos Geraldo Meirelles Palma ISOLA; Fabiano Antonio CARDIOLI; Ana Paula NAKAGE
... Esse estudo comparou a análise hematológica e sorológica no diagnóstico da Ehrlichia canis. A pesquisa da Ehrlichia canis foi feita em esfregaços sanguíneos de 150 cães. O teste sorológico foi realizado em 12 amostras ...
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MATERIALS AND METHODS Experimental
... Rapid screening of antioxidant activity by dot blot assay Antioxidant capacity of fungal extracts was detected semi- quantitatively by a rapid DPPH staining method. This method is typically based on the ...
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Double Acceptor Interaction in Semimagnetic Quantum Dot
... Quantum Dot (QD) leading to the lifting of the degeneracy in the energy ...Quantum Dot plays a vital role on the binding energy of the donor [4] as well as on the diamagnetic susceptibility as the donor ...
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Attentional bias in snus users: an experimental study.
... a dot- probe task to investigate whether 40 snus users show an attentional bias towards snus-relevant stimuli, compared to 40 non-snus ...or dot-probe task, thus failing to show an attentional bias towards ...
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