Electrophoresis, Gel, Pulsed-field
Caracterização de cepas de referência de Leptospira sp utilizando a técnica de pulsed field gel electrophoresis.
... de pulsed ield gel electrophoresis tem sido empregada como uma ferramenta para auxiliar nesta ...sp. Pulsed Field Gel ...
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Multilocus Sequence Typing Scheme versus Pulsed-Field Gel Electrophoresis for Typing Mycobacterium abscessus Isolates
... Outbreaks of infections by rapidly growing mycobacteria following invasive procedures, such as ophthalmological, laparoscopic, arthroscopic, plastic, and cardiac surgeries, mesotherapy, and vaccination, have been ...
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Diversity of Pulsed-Field Gel Electrophoresis Patterns of Mycobacterium abscessus Type 2 Clinical Isolates
... Outbreaks of infections caused by rapidly growing mycobac- teria (RGM) related to surgical and cosmetic procedures have concerned Brazilian health care authorities since 2003 (5, 12, 19, 22–24, 28). More than 2,000 ...
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Avaliação da disseminação de Salmonella Senftenberg isoladas de diversos ambientes avícolas utilizando a técnica de Pulsed Field Gel Electrophoresis
... Nos últimos anos, a emergência de Salmonella Senftenberg no ambiente de granjas de frango tornou-se causa de preocupação, pois este sorotipo, além de ser um potencial agente de paratifo aviário em condições de estresse, ...
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Pseudomonas aeruginosa Endophthalmitis after Penetrating Keratoplasty Transmitted from the Same Donor to Two Recipients Confirmed by Pulsed-Field Gel Electrophoresis
... Two devastating cases of multidrug-resistant Pseudomonas aeruginosa endophthalmitis after keratoplasty as the result of transmission from the same donor were confirmed by pulsed-field gel ...
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Molecular analysis of salmonella anteritidis isolates from the Caribbean by pulsed-field gel electrophoresis
... Of the four countries from which we studied S. enteritidis strains, only one, Trinidad and Tobago, recorded a signif- icant increase in the absolute number of S. enteritidis isolates from 1994 to 1996. Barbados, on the ...
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Genetic heterogeneity of Shiga toxin-producing Escherichia coli strains isolated in São Paulo, Brazil, from 1976 through 2003, as revealed by pulsed-field gel electrophoresis
... The pulsed-field gel electrophoresis (PFGE) patterns of 46 Shiga toxin-producing Escherichia coli (STEC) strains isolated in Sa ˜o Paulo, Brazil, during the period from 1976 to 2003 were ...
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Associated Risk Factors and Pulsed Field Gel Electrophoresis of Nasal Isolates of Staphylococcus aureus from Medical Students in a Tertiary Hospital in Lagos, Nigeria
... and Pulsed field gel electrophoresis is well known in being superior in typeability and reproducibility to any typing methods and allows an excellent discrimination between strains ...
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Genetic profiling of Klebsiella pneumoniae: comparison of pulsed field gel electrophoresis and random amplified polymorphic DNA
... pulsed field gel electrophoresis (PFGE) has been consid- ered as the gold standard for typing of K. pneumoniae (Arlet et al., 1994; Li et al., 2009). Since PFGE is techni- cally demanding, ...
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Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis
... Polymerase chain reaction (PCR) with JB1 or REP consensus oligo- nucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of ...
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Pulsed Field Gel Electrophoresis Reveals Chromosome Length and Number Differences in Brazilian Strains of Metarhizium Anisopliae
... Electrophoretic karyotypes of eight wild-type strains of Metarhizium anisopliae var. anisopliae were obtained by pulsed-field gel electrophoresis. These strains were isolated from insects of ...
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Genetic diversity of Xanthomonas axonopodis pv. citri based on plasmid profile and pulsed field gel electrophoresis
... b) Pulsed Field Gel Electrophoresis (PFGE): Total genomic DNA of X. axonopodis pv. citri strains was ex- tracted by using the protocol described by Cooksey and Graham (1989) and Egel et al. ...
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Single-cell pulsed-field gel electrophoresis to detect the early stage of DNA fragmentation in human sperm nuclei.
... Single-cell pulsed-field gel electrophoresis (SCPFGE) with dual electrode pairs was developed to detect the early stage of DNA fragmentation in human ...
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PODER DISCRIMINATÓRIO DAS TÉCNICAS DE RAPD (Random Amplified Polymorphic DNA) e PFGE (Pulsed-field gel electrophoresis) PARA DIFERENCIAR Campylobacter jejuni ISOLADAS DE CARCAÇAS DE FRANGOS PHELIPE AUGUSTO BORBA MARTINS PERES DAISE APARECIDA ROSSI FAMEV
... ------A análise dos padrões obtidos nos géis pela técnica de PFGE foi realizada utilizando o coeficiente de similaridade de Dice, com tolerância de 1,5% na comparação da posição de bandas. O dendrograma foi construído ...
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Analysis of the clonal diversity of Staphylococcus aureus methicillin-resistant strains isolated at João Pessoa, state of Paraíba, Brazil
... cell wall formation in concentrations of drugs that render the other PBPs inactive (Hartman & Tomasz 1984). The PBP 2a and the gene encoding it (mecA) have been found in all resistant staphylo- cocci (De Lencastre et ...
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Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing
... (pulsed field gel electrophoresis) pattern or belonging to the same clonal group showed differences in the susceptibility to antimicrobial agents (Harris et ...
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Molecular Techniques for MRSA Typing: Current Issues and Perspectives
... Yet another technique based on a Southern type hybridisation is binary typing, which was recently described and is currently used by only one group of researchers [26,37]. In this technique, several probes were made by ...
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Ana Beatriz de A Corrêa, Ivi Cristina M de Oliveira, Tatiana de CA Pinto, Marcos C de Mattos, Leslie C Benchetrit
... Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Pers- ing DH, Swaminathan B 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: ...
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Avaliação comparativa de protocolos rápidos versus protocolos convencionais para tipagem molecular de amostras clinicas de Staphylococcus aureus, Escherichia coli e Pseudomonas aeruginosa pela técnica de pulsed fiedl gel electrophoresis
... "Pulsed Field Gel Electrophoresis" (PFGE), que vem demonstrando ser uma excelente técnica para a tipagem molecular da maioria das espécies bacterianas (OLIVE & BEAN, 1999; ...
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