Gene silencing
Gene silencing mediated by endogenous microRNAs under heat stress conditions in mammalian cells.
... mediated gene silencing under heat-stress conditions. Gene silencing assay with reporter plasmids was carried out in heat- shocked HeLa cells in the presence and absence of geldanamycin that ...
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CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo
... CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in ...
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Gene Silencing and Haploinsufficiency of Csk Increase Blood Pressure.
... Target gene silencing was successfully tested in vivo according to the protocol we have previously ...causative gene for the blood pressure change and furthermore confirmed in a knockout mouse ...
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Combinatorial action of miRNAs regulates transcriptional and post-transcriptional gene silencing following in vivo PNS injury.
... post-transcriptional gene silencing we examined the association of miRNAs and the transcripts of interest with Argonaute-2 (Ago2) protein in vivo after sciatic nerve ...Ago2-mRNA-miRNA silencing ...
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AGO6 functions in RNA-mediated transcriptional gene silencing in shoot and root meristems in Arabidopsis thaliana.
... post-transcriptional gene silencing that involve siRNA-guided cleavage of target mRNAs ...our silencing system, the putative slicer activity of AGO6 is probably not involved extensively in secondary ...
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The post-transcriptional gene silencing pathway in Eucalyptus
... Post-transcriptional gene silencing (PTGS) is a conserved surveillance mechanism that identifies and cleaves double-stranded RNA molecules and their cellular cognate ...RNA silencing response is ...
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The Role of Apollon Gene Silencing on Viablity and Radiosensitivity of Cervical Cancer Hela Cells
... In previous study, we primarily have seen that expression of shRNAs for apollon markedly reduces the mRNA levels of this gene by mean factor of 0.02 in transfected hela Cell (Milani et al,2016). In this study, we ...
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Targeted gene-silencing reveals the functional significance of myocardin signaling in the failing heart.
... myocd silencing in non-failing piglet heart in ...cardiac gene transfer following injection via the transcutaneously inserted catheter (see Materials and Methods) has been previously reported ...this ...
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Both strands of siRNA have potential to guide posttranscriptional gene silencing in mammalian cells.
... Despite the widespread application of RNA interference (RNAi) as a research tool for diverse purposes, the key step of strand selection of siRNAs during the formation of RNA-induced silencing complex (RISC) ...
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Identification of Multiple Proteins Coupling Transcriptional Gene Silencing to Genome Stability in Arabidopsis thaliana.
... AtSAC3B gene in the ems_2_37 (atsac3b-4) ...(F) Gene structure of AtSAC3B and AtTHP1 showing newly identified point mutations from EMS mutagenesis as well as insertional mutants (triangles) used for ...
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MECHANISMS OF GENE SILENCING
... RNA silencing pathways (Merai et ...of gene expression in virus hosts, since they affect both miRNA and siRNA pathways, leading to developmental abnormalities and possibly vi- ral symptoms (Voinnet, ...p23 ...
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Evaluation of Morpholino Antisense Oligos’ Role on BCR-ABL Gene Silencing in the K562 Cell Line
... Objective: Chronic myeloid leukemia (CML) develops when a hematopoietic stem cell acquires the BCR/ABL fusion gene. This causes these transformed hematopoietic cells to have a greater than normal proliferation ...
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Mobile gene silencing in Arabidopsis is regulated by hydrogen peroxide
... altered silencing movement in two independent systems: our root-to-shoot systemic silencing system; RtSS, and the cell-to-cell short distance silencing system; and ...of silencing signal ...
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Gold nanoparticle-DNA conjugates for oligonucleotide vectorization towards gene silencing
... 6 Antisense oligonucleotides and siRNA show significant potential in new molecular approaches to down-regulate specific gene expression in cancer. However, there are still significant obstacles to be overcome ...
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The Anti-Adipogenic Potential of COUP-TFII Is Mediated by Downregulation of the Notch Target Gene Hey1.
... Coup-tfII gene silencing was significantly higher as compared to controls (Table ...Coup-tfII gene silencing, and quantitative analysis confirmed com- parable macrophage ...
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TAK1 is required for survival of mouse fibroblasts treated with TRAIL, and does so by NF-kappaB dependent induction of cFLIPL.
... arise from mRNA splicing, and the cleaved form, Flipp43 [3,4]. All these variants of cFlip bear two DED domains but only cFlipL possesses a caspase-like domain, which lacks catalytic activity. Therefore, all cFlip forms ...
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Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response
... filaggrin gene silencing in normal human epidermal keratinocytes (NHEKs) could induce Th2-pre- dominant ...filaggrin gene in NHEKs, detected the expression of proteins related to skin barrier ...
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α-Fetoprotein promoter-driven Cre/LoxP-switched RNA interference for hepatocellular carcinoma tissue-specific target therapy.
... target gene in an HCC-specific ...target gene while exerting its effect within target cells or ...1 gene driven by AFP promoter or CMV promoter (AFP-miRNA and ...target gene (Beclin 1) of HCC ...
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Hist. cienc. saudeManguinhos vol.20 número4
... of gene silencing were developed a few years later, it was because the scientific capital in transgenesis had already been won by other researchers, so that scientists new to the field needed to ...
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Relationship between symptoms and gene expression induced by the infection of three strains of Rice dwarf virus.
... host gene expression compared among Nicotiana plants infected with some RNA viruses such as Cymbidium ringspot virus, Turnip crinkle virus, Ribgrass mosaic virus, and Cucumber mosaic virus (CMV) showed that the ...
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