High-throughput sequencing
Direct metagenomic detection of viral pathogens in nasal and fecal specimens using an unbiased high-throughput sequencing approach.
... parallel sequencing platform for viral detection in nasopharyngeal and fecal samples collected during seasonal influenza virus (Flu) infections and norovirus outbreaks from 2005 to 2007 in Osaka, ...Unbiased ...
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Exploring the sources of bacterial spoilers in beefsteaks by culture-independent high-throughput sequencing.
... culture-independent high-throughput sequencing of 16S rRNA gene ...initially high complexity of the carcass microbiota, the steaks after aerobic storage at 4uC showed a dramatic decrease in ...
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Quantitative analysis of focused a-to-I RNA editing sites by ultra-high-throughput sequencing in psychiatric disorders.
... DNA sequencing approach, Li et al [24] detected several hundred l RNA editing sites by comparing genomic DNA with RNA from seven tissues of one ...Sanger sequencing methods ...
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Detection of genomic variation by selection of a 9 mb DNA region and high throughput sequencing.
... for high throughput ...and sequencing resulted in 260-fold enrichment, with 41% of reads mapping to the target ...Sanger sequencing were performed for 46 SNPs with 15-20 fold coverage, with a ...
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A method enabling high-throughput sequencing of human cytomegalovirus complete genomes from clinical isolates.
... IGA sequencing has a higher throughput and lower cost per base and therefore achieves much higher coverages than 454 GS FLX sequencing in this study (Table ...FLX sequencing in homopolymeric ...
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PCR Strategies for Complete Allele Calling in Multigene Families Using High-Throughput Sequencing Approaches.
... Our study identified amplification biases derived from large differences in amplification efficiencies among targets as a major limitation for the characterization of multigene families through the ...
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Bulk segregant analysis by high-throughput sequencing reveals a novel xylose utilization gene from Saccharomyces cerevisiae.
... Fermentation of xylose is a fundamental requirement for the efficient production of ethanol from lignocellulosic biomass sources. Although they aggressively ferment hexoses, it has long been thought that native ...
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A bioinformatics approach for determining sample identity from different lanes of high-throughput sequencing data.
... any high throughput ...As high throughput sequencing becomes commoditized, such an approach will be increasingly important for validating large sequencing ...
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Large-Scale Biomonitoring of Remote and Threatened Ecosystems via High-Throughput Sequencing.
... a high-throughput DNA sequencing approach could be used to determine biodiversity metrics from bulk envi- ronmental samples collected as part of a large-scale biomonitoring ...The ...
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Identification and characterization of microRNAs from peanut (Arachis hypogaea L.) by high-throughput sequencing.
... and sequencing is the most effective method available for the discovery of plant ...of high- throughput sequencing methods, such as the 454 Technology and the Solexa platform, has greatly ...
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High-throughput sequencing analysis of post-liver transplantation HCV E2 glycoprotein evolution in the presence and absence of neutralizing monoclonal antibody.
... Chronic hepatitis C virus (HCV) infection is the most common cause of end-stage liver disease, often leading to liver transplantation, in which case circulating virions typically infect the transplanted liver within ...
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Identification and Characterization of MicroRNAs from Longitudinal Muscle and Respiratory Tree in Sea Cucumber (Apostichopus japonicus) Using High-Throughput Sequencing.
... the high-through sequencing method were reported in recent ...performed high-throughput sequencing to identify differentially expressed miRNAs in the intestine between normal and ...
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Identification and analysis of red sea mangrove (Avicennia marina) microRNAs by high-throughput sequencing and their association with stress responses.
... using high-throughput sequencing is, in most cases, a straightforward process for model plants for which genomic and biocomputing tools are widely ...deep sequencing approach to miRNA ...
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High Throughput Sequencing Identifies Misregulated Genes in the Drosophila Polypyrimidine Tract-Binding Protein (hephaestus) Mutant Defective in Spermatogenesis.
... a high throughput sequencing approach for transcriptome profiling and have identi- fied transcripts that are misregulated in the heph 2 ...conserved high affinity PTB binding site near a ...
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Human skin microbiota: high diversity of DNA viruses identified on the human skin by high throughput sequencing.
... Viral colonization of healthy skin was primarily and extensively described for papillomaviruses and the commensal nature of these viruses is now a widely admitted data. Indeed, the presence of resident HPVs on normal ...
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Ultra high throughput sequencing in human DNA variation detection: a comparative study on the NDUFA3-PRPF31 region.
... of cost and time that represent a fraction of what is usually spent for conventional sequencing. Furthermore, our successful adoption of a user-friendly software and a straightforward analytical pipeline ...
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Analysis of high-throughput sequencing and annotation strategies for phage genomes.
... dsDNA High Sensitivity Assay Kit, Invitrogen, Carlsbad CA), and subsequently incubated in the presence of a 300-fold molar excess of BstXI/EcoRI adaptor (Invitrogen, Carlsbad CA), 10 U Fast- Link DNA Ligase and ...
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Exploration of immunoglobulin transcriptomes from mice immunized with three-finger toxins and phospholipases A2 from the Central American coral snake, Micrurus nigrocinctus
... employ high-throughput sequencing of the immunoglobulin (Ig) transcriptome of mice immunized with a three-finger toxin and a phospholipase A 2 from the venom of the Central American coral snake, ...
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Seasonal variations of microbial community in a full scale oil field produced water treatment plant
... and high throughput sequencing, revealed that the bacteria related to organic matter degradation belong to different taxonomic groups, including Methylobacterium ...
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The use of high-throughput DNA sequencing in the investigation of antigenic variation: application to Neisseria species.
... by sequencing of each amplicon using Sanger sequencing technology to detect the subset that contained variant sequences ...that high throughput sequencing of DNA extracted from a ...
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