Pulsed-field gel electrophoresis (PFGE)
Caracterização de cepas de referência de Leptospira sp utilizando a técnica de pulsed field gel electrophoresis.
... de pulsed ield gel electrophoresis tem sido empregada como uma ferramenta para auxiliar nesta ...sp. Pulsed Field Gel ...
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Genetic profiling of Klebsiella pneumoniae: comparison of pulsed field gel electrophoresis and random amplified polymorphic DNA
... of pulsed field gel electrophoresis (PFGE) and random ampli- fied polymorphic DNA (RAPD) methods for subtyping of 54 clinical isolates of Klebsiella pneumoniae were ...
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Pulsed Field Gel Electrophoresis Reveals Chromosome Length and Number Differences in Brazilian Strains of Metarhizium Anisopliae
... among soil isolates, suggesting that this fungus was developed a certain degree of host specificity. An effective technique designated pulsed-field gel electrophoresis (PFGE), was used to ...
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Genetic diversity of Xanthomonas axonopodis pv. citri based on plasmid profile and pulsed field gel electrophoresis
... b) Pulsed Field Gel Electrophoresis (PFGE): Total genomic DNA of X. axonopodis pv. citri strains was ex- tracted by using the protocol described by Cooksey and Graham (1989) and Egel et al. ...
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Diversity of Pulsed-Field Gel Electrophoresis Patterns of Mycobacterium abscessus Type 2 Clinical Isolates
... An epidemic of infections by rapidly growing mycobacteria related to surgical procedures between 2004 and 2008 in Brazil was caused by a unique strain showing the Mycobacterium abscessus type 2 pattern when it was ...
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Time-based distribution of Staphylococcus saprophyticus pulsed field gel-electrophoresis clusters in community-acquired urinary tract infections
... The epidemiology of urinary tract infections (UTI) by Staphylococcus saprophyticus has not been fully char- acterised and strain typing methods have not been validated for this agent. To evaluate whether epidemiological ...
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Avaliação da disseminação de Salmonella Senftenberg isoladas de diversos ambientes avícolas utilizando a técnica de Pulsed Field Gel Electrophoresis
... Nos últimos anos, a emergência de Salmonella Senftenberg no ambiente de granjas de frango tornou-se causa de preocupação, pois este sorotipo, além de ser um potencial agente de paratifo aviário em condições de estresse, ...
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Molecular analysis of salmonella anteritidis isolates from the Caribbean by pulsed-field gel electrophoresis
... Using pulsed-field gel electrophoresis (PFGE), between 1987 and 1996 we analyzed Salmo- nella enteritidis isolates from gastroenteritis cases in four Caribbean countries: Barbados, Saint Kitts ...
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Pseudomonas aeruginosa Endophthalmitis after Penetrating Keratoplasty Transmitted from the Same Donor to Two Recipients Confirmed by Pulsed-Field Gel Electrophoresis
... The corneoscleral rim tissues were sent to the laboratory and cultured in Sabouraud medium (kept at room temperature for 30 days) and in thioglycolate medium (kept in an incubator at 37°C for 24 h) as part of our ...
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Associated Risk Factors and Pulsed Field Gel Electrophoresis of Nasal Isolates of Staphylococcus aureus from Medical Students in a Tertiary Hospital in Lagos, Nigeria
... the pulsed field gel patterns of the strains, nasal swabs were collected from 185 medical students in a teaching hospital in Lagos, ...and Pulsed Field Gel Electrophoresis ...
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Multilocus Sequence Typing Scheme versus Pulsed-Field Gel Electrophoresis for Typing Mycobacterium abscessus Isolates
... control. Pulsed-field gel electrophoresis (PFGE) is usually performed when specific methods are not available for the species being stud- ...
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Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis
... Polymerase chain reaction (PCR) with JB1 or REP consensus oligo- nucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of ...
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Genetic heterogeneity of Shiga toxin-producing Escherichia coli strains isolated in São Paulo, Brazil, from 1976 through 2003, as revealed by pulsed-field gel electrophoresis
... The pulsed-field gel electrophoresis (PFGE) patterns of 46 Shiga toxin-producing Escherichia coli (STEC) strains isolated in Sa ˜o Paulo, Brazil, during the period from 1976 to 2003 were ...
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Single-cell pulsed-field gel electrophoresis to detect the early stage of DNA fragmentation in human sperm nuclei.
... Single-cell pulsed-field gel electrophoresis (SCPFGE) with dual electrode pairs was developed to detect the early stage of DNA fragmentation in human ...
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PODER DISCRIMINATÓRIO DAS TÉCNICAS DE RAPD (Random Amplified Polymorphic DNA) e PFGE (Pulsed-field gel electrophoresis) PARA DIFERENCIAR Campylobacter jejuni ISOLADAS DE CARCAÇAS DE FRANGOS PHELIPE AUGUSTO BORBA MARTINS PERES DAISE APARECIDA ROSSI FAMEV
... ------A análise dos padrões obtidos nos géis pela técnica de PFGE foi realizada utilizando o coeficiente de similaridade de Dice, com tolerância de 1,5% na comparação da posição de bandas. O dendrograma foi construído ...
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Pulsed-field gel electrophoresis (PFGE) analysis of Listeria monocytogenes isolates from different sources and geographical origins and representative of twelve serovars
... although pulsed-field gel electrophoresis (PFGE) is the current gold standard method for molecular subtyping of most foodborne bacteria, including ...
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Avaliação comparativa de protocolos rápidos versus protocolos convencionais para tipagem molecular de amostras clinicas de Staphylococcus aureus, Escherichia coli e Pseudomonas aeruginosa pela técnica de pulsed fiedl gel electrophoresis
... "Pulsed Field Gel Electrophoresis" (PFGE), que vem demonstrando ser uma excelente técnica para a tipagem molecular da maioria das espécies bacterianas (OLIVE & BEAN, 1999; ...
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Serum protein concentration in newborn Holstein calves determined by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis.
... em gel de acrilamida contendo dodecil sulfato de sódio (SDS-PAGE) é relativamente simples e de baixo custo, possibilitando a visualização de concentrações séricas extremamente baixas e a identificação de várias ...
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A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
... Two-dimensional gel electrophoresis (2DE) is an extremely powerful tool to dissect multiprotein complexes. No other technique can resolve so many protein subunits in a single operation, which is possible by ...
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