RNA silencing
The RNA silencing pathway: the bits and pieces that matter.
... in RNA silencing phenomena in different species ...molds, silencing can be induced by transgenes, IRs, transposons, and ...induced silencing, while virus (dsRNA) induced silencing is ...
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Olive Mild Mosaic Virus Coat Protein and P6 are suppressors of RNA silencing, and their silencing confers resistance against OMMV
... Abstract: RNA silencing is an important defense mechanism in plants, yet several plant viruses encode proteins that suppress this ...OMMV RNA. When silencing of both suppressors was achieved, ...
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Genetic variability and evolutionary implications of RNA silencing suppressor genes in RNA1 of sweet potato chlorotic stunt virus isolates infecting sweetpotato and related wild species.
... of RNA silencing by the p22 and RNase3 proteins of SPCSV isolates and the RNase3-like protein of the new virus ...“strong silencing” of the gfp gene for green fuorescent protein (GFP) was achieved by ...
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The VP3 factor from viruses of Birnaviridae family suppresses RNA silencing by binding both long and small RNA duplexes.
... RNA silencing collectively refers to diverse gene expression regulatory pathways that control a large number of cellular processes, such as developmental patterning, responses to biotic and abiotic ...
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Genetic machinery for RNA silencing and defense against viruses in Citrus
... RNA silencing is a ubiquitous and highly conserved phenomenon throughout the evolution of eukaryotes (Met- te et ...which RNA can be silenced in plant cells: i) Post-Transcriptional Gene ...
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Short Hairpin RNA Silencing of PHD-2 Improves Neovascularization and Functional Outcomes in Diabetic Wounds and Ischemic Limbs.
... Isolated RNA was quantified using Nanodrop 2000 (Thermo Fisher Scientific, Waltham, MA) then reverse- transcribed using TaqMan Reverse Transcription Reagents ...
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The post-transcriptional gene silencing pathway in Eucalyptus
... the RNA silencing mechanism among different plant species and ...that RNA silencing and related pathways are involved in several cellular pro- cesses such as defense, RNA surveillance ...
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MECHANISMS OF GENE SILENCING
... both RNA and DNA viruses is the incorporation of silenc- ing suppressor proteins into their genomes (Moissiard & Voinnet, ...the RNA silencing pathways (Merai et ...
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High Temperature, High Ambient CO₂ Affect the Interactions between Three Positive-Sense RNA Viruses and a Compatible Host Differentially, but not Their Silencing Suppression Efficiencies.
... assess silencing suppression separately from other viral processes is the use of virus-free agro- patch assays, which can compare silencing suppression strength by measuring the transient steady-state ...
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Nicotiana small RNA sequences support a host genome origin of cucumber mosaic virus satellite RNA.
... RNA silencing is an evolutionarily conserved gene regulation mechanism in eukaryotes mediated by 20-25-nt small RNAs (sRNAs) ...(hp) RNA by Dicer or Dicer-like (DCL) protein. To induce ...
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The RNA interference revolution
... RNA silencing has long been recognized as part of the immune response against vi- ruses in plants (1), but the RNA interference (RNAi) revolution started with the observa- tions that sense plus ...
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The impact of mRNA structure on guide RNA targeting in kinetoplastid RNA editing.
... Procyclic-form mitochondria were isolated, lysed and cleared by centrifugation. The extract (,2610 10 cell equivalents/ml) was then separated by glycerol gradient sedimentation as previously described [16]. We ...
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Revealing potential long non-coding RNA biomarkers in lung adenocarcinoma using long non-coding RNA-mediated competitive endogenous RNA network
... SNHG12 is a member of small nucleolar RNA host genes (SNHGs), which have been indicated to contribute to cancer progression. Ruan et al. (37) have demonstrated that SNHG12 contributes to cell proliferation and ...
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Desenvolvimento de um sistema de genética reversa para tospovírus via moléculas de RNA defectivo-interferente (DI-RNA)
... 1992) para descrever as propriedades físico-químicas da proteína L de TCSV. Os resultados produzidos por três diferentes algoritmos de busca de estruturas secundárias em sequências de aminoácidos – GOR4, HNN e SOPMA ...
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Potent host-directed small-molecule inhibitors of myxovirus RNA-dependent RNA-polymerases.
... strand RNA virus (sindbis virus of the Alphaviridae) and DNA virus (vaccinia virus of the Poxviridae) families (figure 1C, inhibitory concentrations for a larger panel of myxovirus family members are summarized in ...
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RNA CoMPASS: a dual approach for pathogen and host transcriptome analysis of RNA-seq datasets.
... In RNA CoMPASS, we have implemented both the Java Parallel Processing Framework (JPPF) API and Portable Batch System (PBS) API in order to deploy it on either a small local cluster or a grid system managed by PBS ...
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Detection theory in identification of RNA-DNA sequence differences using RNA-sequencing.
... Next, we investigated the percentage of observed RDDs that are removed by filters we previously identified as effective in reducing the amount of false positive RDDs. Other researchers have used these filters in their ...
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Gene Silencing and Haploinsufficiency of Csk Increase Blood Pressure.
... their silencing efficacy, 20 nM of the siRNAs was transfected into B16F10 cells and NIH3T3 cells using G-Fec- tin (Genolution, Seoul, Korea) and Lipofectamine 2000 (Invitrogen, Carlsbad, CA) respectively according ...
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Silencing by H-NS potentiated the evolution of Salmonella.
... H-NS is an abundant DNA-binding protein found in enteric bacteria including the important pathogens Escherichia, Salmonella, Vibrio, and Yersinia, that plays a primary role in defending the bacterial genome by ...
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Gold nanoparticle-DNA conjugates for oligonucleotide vectorization towards gene silencing
... Besides lowering the level of in vitro transcription, the addition of these oligonucleotides in the reaction had an additional effect: several bands with lower molecular weights than the mRNA product are also visible in ...
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