RT-qPCR
Assessment of RT-qPCR normalization strategies for accurate quantification of extracellular microRNAs in murine serum.
... green RT-qPCR array platform we were able to directly assess three different normalization ...741 RT-qPCR assays of which 124 were detected in all samples (Table ...
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Expression stabilities of candidate reference genes for RT-qPCR under different stress conditions in soybean.
... TUB4 when the data were analyzed by NormFinder, but it was changed to be UKN2 when GeNorm was used, although CYP was found to be the most unstable gene using either of the two softwares (Fig. 2; Table 3). These results ...
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Expression Stabilities of Candidate Reference Genes for RT-qPCR in Chinese Jujube (Ziziphus jujuba Mill.) under a Variety of Conditions.
... 5.0. RT-qPCR was performed with a Bio-Rad iQ™5 using TransStart Top Green qPCR SuperMix AQ131 (TransGen Biotech, ...for RT-qPCR was preincubation for 30 ...
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Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp
... during RT-qPCR ...for RT-qPCR analysis of target gene expression in the hepatopancreas of crucian carp (Carassius auratus) under various conditions (sex, age, wa- ter temperature, and drug ...
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Expressão dos genes nodC, nodW e nopP em Bradyrhizobium japonicum estirpe CPAC 15 avaliada por RT-qPCR.
... by RT-qPCR, the expression of the nodC and nodW nodulation genes and of the nopP gene of the CPAC 15 strain, which probably play a role in the infection of soybean ...
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Canine distemper virus detection by different methods of One-Step RT-qPCR
... One-Step RT-qPCR (System A) and One-Step RT-qPCR combined with NESTED- qPCR (System B) were compared using the RT- qPCR kit I, which showed better dissociation curves, ...
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Selection of reference genes for RT-qPCR studies in blood of beluga whales (Delphinapterus leucas)
... Reverse transcription quantitative PCR (RT-qPCR) is used for research in gene expression, and it is vital to choose appropriate housekeeping genes (HKGs) as reference genes to obtain correct results. The ...
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Aplicação de conjugado de microesferas de poliestireno para preparo de RNA no diagnóstico da cinomose canina por RT-qPCR
... One-Step RT-qPCR of viral RNA extracted from samples centrifuged at 8,000 x g for 10 minutes revealed a higher quantity of RNA virus (about tree fold), most probably due to infected cell sedimentation ...
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Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
... Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) RNA was extracted using the UNIQ-10 column TRIzol kit (Shanghai Sangon Biotech, Shanghai, China) and treated with DNase. A total of 1 µ ...
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Selection of suitable reference genes for RT-qPCR analyses in cyanobacteria.
... of RT-qPCR experiments can lead to erroneous or biologically irrelevant data ...efficiency, qPCR protocol and validation have been shown to compromise the quality of the final RT-qPCR ...
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Design of a custom RT-qPCR array for assignement of abiotic stress tolerance in traditional portuguese grapevine varieties
... custom-made RT-qPCR array to assess the sensitivity of traditional Portuguese grapevine varieties to abiotic stresses was elaborated based on previously obtained data from well-studied varieties (Rocheta et ...
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INFLUÊNCIA DAS CONDIÇÕES DE ARMAZENAMENTO DO VÍRUS INFLUENZA NA DETECÇÃO POR RT-qPCR E ISOLAMENTO VIRAL INFLUENCE OF STORAGE CONDITIONS OF INFLUENZA VIRUS ON VIRUS DETECTION BY RT-qPCR AND VIRAL ISOLATION
... A infecção de suínos pelo vírus influenza causa perdas significativas na suinocultura e a doença tem implicações consideráveis para a saúde pública. Dessa forma, a rápida detecção viral em amostras biológicas de suínos é ...
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UNIVERSIDADE NOVA DE LISBOA INSTITUTO DE HIGIENE E MEDICINA TROPICAL A RT – qPCR como ferramenta de diagnóstico da infecção por Pneumocystis jirovecii
... (2013) Pneumocystis jirovecii colonization in non- HIV-infected patients based on nested-PCR detection in bronchoalveolar lavage samples. (2014) Prognostic factors of Pneu[r] ...
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Dicer-2-dependent activation of Culex Vago occurs via the TRAF-Rel2 signaling pathway.
... Interestingly, Rel2 is also activated by the Imd pathway in response to gram-negative bacterial infection in insects [32]. Peptidoglycans on gram-negative bacteria are recognized by PGRP receptor in insects, which leads ...
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J. Appl. Oral Sci. vol.22 número3
... O bjective: The aim of this study was to evaluate a possible synergism between AGe- RAGe and TLR4 signaling and the role of p38 MAPK and NF-kB signaling pathways on the modulation of the expression of inlammatory ...
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Identification of thalidomide-specific transcriptomics and proteomics signatures during differentiation of human embryonic stem cells.
... The differential expression in heart and limb development BP coincides with clinical evidence of thalidomide toxicity [9,10]. The representative genes from the respective BP uncovered transcrip- tional factors (such as ...
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Dual regulation of myocardin expression by tumor necrosis factor-α in vascular smooth muscle cells.
... and RT-qPCR analysis show the effect of QNZ and PD98059 on TNFa-induced increase in myocardin and contractile marker protein and mRNA expression levels compared with the control (C) in differentiated ...
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Glutathione S-Transferase Regulation in Calanus finmarchicus Feeding on the Toxic Dinoflagellate Alexandrium fundyense.
... Two independent methods (RT-qPCR and RNA-Seq) were used to examine changes in the expression of GST genes in C. finmarchicus in response to the dinoflagellate A. fundyense. Results from RNA-Seq and ...
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The co-repressor SMRT delays DNA damage-induced caspase activation by repressing pro-apoptotic genes and modulating the dynamics of checkpoint kinase 2 activation.
... The qPCR was performed on the immunoprecipitated DNA, and percentage of the input was calculated by comparing the sample Ct with a curve made of four serial 1/5 dilutions of 1% ...and qPCR with specific ...
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Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions.
... of RT-qPCR in papaya, the present study aimed at defining reference genes suited for quantitative analysis of papaya genes under different experimental ...with RT-qPCR analysis in ...for ...
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