3T3-L1
Triiodothyronine Increases mRNA and Protein Leptin Levels in Short Time in 3T3-L1 Adipocytes by PI3K Pathway Activation
... adipocytes, 3T3-L1, cell ...treating 3T3-L1 adipocytes with physiological (P=10nM) or supraphysiological (SI=100 nM) T3 dose during one hour (short time), in the absence or the presence of ...
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Both adiponectin and interleukin-10 inhibit LPS-induced activation of the NF-kappa B pathway in 3T3-L1 adipocytes
... Almost all TLRs have a common signaling pathway in which MYD88 adaptor molecules form a molecular complex with TLR- initiated signaling events. MYD88 also interacts with downstream IL-1R-associated kinase (IRAKs) [26], ...
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In vitro and in vivo enhancement of adipogenesis by Italian ryegrass (Lolium multiflorum) in 3T3-L1 cells and mice.
... Italian Ryegrass (IRG) is being cultivated in South Korea to produce silage for domestic animals. Most of the farmers in South Korea have cultivated IRG as a feed for ruminants, because of its higher nutritive value and ...
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Phosphorylation of mRNA decapping protein Dcp1a by the ERK signaling pathway during early differentiation of 3T3-L1 preadipocytes.
... In Saccharomyces cerevisiae, Dcp2p interacts directly with Dcp1p, and this interaction is required for decapping in vivo and in vitro [19,20,24]. The crystal structure of free Dcp2p from Schizosacchar- omyces pombe ...
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Mammalian ste20-like kinase and SAV1 promote 3T3-L1 adipocyte differentiation by activation of PPARγ.
... confluent 3T3-L1 cells are exposed to differentiation stimuli, they undergo several rounds of cell division, called mitotic clonal expansion, followed by cell cycle exit to enter the terminal ...
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Regulation of myosin light chain kinase during insulin-stimulated glucose uptake in 3T3-L1 adipocytes.
... Another signaling pathway regulating insulin-stimulated glucose uptake is the CaMKII pathway [39,46]. CaMKII is a Ca 2+ - dependent kinase with multiple substrates which include ERK2 and MLCK [38,39]. CaMKII has four ...
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Blueberry peel extracts inhibit adipogenesis in 3T3-L1 cells and reduce high-fat diet-induced obesity.
... Despite the short-term benefits of treating obesity with drugs, medication-induced weight loss is often associated with negative side effects and rebound weight gain when the medications are discontinued [13]. Thus, new ...
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Regulation of Adipogenesis and Key Adipogenic Gene Expression by 1, 25-Dihydroxyvitamin D in 3T3-L1 Cells.
... To better understand the expression pattern of PPARγ during the process of adipocyte differ- entiation, RNA extracts and protein extracts from 3T3-L1 cells treated with DM only were ob- tained for real-time ...
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Catabolism of Branched Chain Amino Acids Contributes Significantly to Synthesis of Odd-Chain and Even-Chain Fatty Acids in 3T3-L1 Adipocytes.
... metabolism. 3T3-L1 adipocytes are an impor- tant in vitro model system for adipose tissue and are commonly used as this cell line has similar phenotypic behavior as adipose ...of 3T3-L1 ...
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Effects of varying degrees of intermittent hypoxia on proinflammatory cytokines and adipokines in rats and 3T3-L1 adipocytes.
... NF-kB 3T3-L1 adipocytes harvested from each preparation were washed twice with ice-cold phosphate-buffered saline (PBS) and the cell suspension was transferred into a centrifuge ...
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Regulação da adipogênese e da secreção de quemerina por ácidos graxos de cadeia média e pelo ácido graxo eicosapentaenóico em células 3T3-L1
... preadipócitos 3T3-L1 foram incubados em meio basal (DMEM a 25 mM de glicose, 10% v/v de FBS, 100 unidades/mL de penicilina e 100 µg/mL de estreptomicina), durante todo o processo de ...
118
17b-estradiol aumenta a expressão de Slc2a4/GLUT4 em adipócitos 3T3-L1 via ESR1
... Insulin resistance (IR) is the reduced ability of insulin sensitive tissues to respond to regular levels of the hormone. In these tissues, the glucose transporter GLUT4 (Slc2a4 gene) is in charge of the insulin ...
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Supplementing alpha-tocopherol (vitamin E) and vitamin D3 in high fat diet decrease IL-6 production in murine epididymal adipose tissue and 3T3-L1 adipocytes following LPS stimulation
... Background: It is well known that high fat diets (HFDs) induce obesity and an increase in proinflammatory adipokines. Interleukin-6 (IL-6) is considered the major inflammatory mediator in obesity. Obesity is associated ...
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The Effect of Pericellular Oxygen Levels on Proteomic Profile and Lipogenesis in 3T3-L1 Differentiated Preadipocytes Cultured on Gas-Permeable Cultureware.
... Cultured 3T3-L1 cells are widely used as a model in adipocyte research due to their ability to differentiate and accumulate triglycerides in lipid droplets, and due to their structural and met- abolic ...
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Avaliação do perfil de expressão gênica modulado pelo GQ-16 em adipócitos 3T3-L1 utilizando a técnica de microarranjo
... células 3T3-L1 revelaram que o diclofenaco sódico, indometacina e ibuprofeno são capazes de induzir a diferenciação dessas células em adipócitos, embora com intensidades ...adipócitos 3T3-L1 ...
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Triiodotironina modula a expressão de leptina e adiponectina em adipócitos 3T3-L1.
... A figura 3 mostra a modulação dos níveis de mRNA de adiponectina em adipócitos, 3T3-L1, na ausência (C) e presença do T3 (F, SI e SII, respectivamente) em dife- rentes períodos de tempo (0,5, 6 e 24 horas) ...
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Diferentes doses de Triiodotironina (T3) aumentam a expressão gênica de leptina, adiponectina e TRα com o envolvimento da via fosfatidil inositol 3 quinase (PI3K) em adipócitos, 3T3-L1
... adipocytes, 3T3-L1, cell ...cells 3T3-L1 will be incubated by one hour with T3 in the presence or absence of the inhibitors mentioned ...adipocytes 3T3-L1 and PI3K signaling ...
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Chito-oligosaccharide inhibits the de-methylation of a 'CpG' island within the leptin (LEP) promoter during adipogenesis of 3T3-L1 cells.
... Mouse 3T3-L1 cells were stimulated to differentiate in the absence or presence of COS and the levels of leptin mRNA and protein were evaluated on days 0, 2, 4 and 6 post-induction of differentiation ...
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TNF-alpha modulates statin effects on secretion and expression of MCP-1, PAI-1 and adiponectin in 3T3-L1 differentiated adipocytes
... line 3T3-L1, in the 11th passage were stored in liquid nitrogen, at -120 °C until thawed, plated and grown in DMEM-HG (Dulbecco’s Modified Eagle Medium high glucose, Gibco) culture medium supplemented with ...
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Phyllostachys edulis compounds inhibit palmitic acid-induced monocyte chemoattractant protein 1 (MCP-1) production.
... on 3T3-L1 cells with the same PA-induced inflammatory model to determine whether BEX contained specific compounds that inhibited MCP-1 ...confluent 3T3-L1 cells treated for 20 hours with PA in ...
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