TRANSGENIC ZEBRAFISH
Transgenic zebrafish recapitulating tbx16 gene early developmental expression.
... creating transgenic fish expressing GFP under the control of the tbx16 ...of transgenic zebrafish were generated by injecting linearised construct into single-cell ...fish. Transgenic F2 ...
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Generation of new transgenic zebrafish lines for studying neuronal circuits underlying behavior in zebrafish
... behavior. Zebrafish has become a promising model organism in the study of nervous ...new transgenic zebrafish lines, through the Tol2 transposon ...in zebrafish to understand how behaviors are ...
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In Vivo Screening Using Transgenic Zebrafish Embryos Reveals New Effects of HDAC Inhibitors Trichostatin A and Valproic Acid on Organogenesis.
... In zebrafish, a treatment with atrazine 20 μM just after fertilization could disturb the normal development (blood circulation and delays) ...nkx2.2a-mEGFP transgenic line, a neurotoxic effect was also ...
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A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach
... GH transgenic - were obtained from Universidade Federal do Rio Grande (Rio Grande, RS, ...GH transgenic zebraish belong to the F0104 line that has a carp (Cyprinus carpio) β-actin promoter driving the ...
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Imaging transient blood vessel fusion events in zebrafish by correlative volume electron microscopy.
... We set out to develop and apply volume EM techniques to study another highly complex three-dimensional network, that of the developing circulatory system. The formation of new blood vessels, angiogenesis, is crucial in ...
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Synergistic Induction of Potential Warburg Effect in Zebrafish Hepatocellular Carcinoma by Co-Transgenic Expression of Myc and xmrk Oncogenes.
... Xmrk transgenic zebrafish, Tg(fabp10:TA; TRE:xmrk; krt4:GFP) or TO(xmrk), and Myc transgenic zebrafish, Tg(fabp10: TA; TRE:Myc; krt4:GFP) or TO(Myc), have been previously described [7, ...
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Two types of Tet-On transgenic lines for doxycycline-inducible gene expression in zebrafish rod photoreceptors and a gateway-based tet-on toolkit.
... stable transgenic zebrafish line that expresses doxycycline (Dox)-inducible GFP specifically in rod ...when transgenic larvae are treated for 72 h with Dox (3–6 ...
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A dominant negative zebrafish Ahr2 partially protects developing zebrafish from dioxin toxicity.
... Construction and Validation of dnAhr2 Transgenics We chose Ahr2 1–440 , and Ahr2 Eng , the two strongest dnAhr2s, to make transgenic zebrafish lines expressing the dnAhr2s from the Xenopus laevis EF1-alpha ...
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Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation.
... The Tol2 transposon/transposase system [41, 56–59] was employed for the establishment of transgenic zebrafish. The coding sequence of lbx1b was cloned into pME-MCS to generate pME-lbx1b. The complementary ...
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Identification of Chemical Inhibitors of β-Catenin-Driven Liver Tumorigenesis in Zebrafish.
... adult zebrafish over time, we raised and main- tained 5 clutches of Tg(fabp10a:pt-β-cat) s987 zebrafish and parallel tanks of non-transgenic con- trol ...point, zebrafish were randomly ...
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Beyond the whole-mount phenotype: high-resolution imaging in fluorescence-based applications on zebrafish
... in transgenic zebrafish, as well as in immunohistochemistry, cell tracking and enzyme histochemistry, with preservation of fluorescent signals at the tissue and cellular ...
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The zebrafish operculum: a powerful system to assess osteogenic bioactivities of molecules with pharmacological and toxicological relevance
... the zebrafish operculum to screen for molecules with osteogenic ...of transgenic zebrafish lines, for osteoblast- specific marker genes, has proven valuable for gaining insight into the cellular ...
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Expression of Rice CYP450-Like Gene (Os08g01480) in Arabidopsis Modulates Regulatory Network Leading to Heavy Metal and Other Abiotic Stress Tolerance.
... The predicted network (Fig 5) shows that the genes involved in many cellular processes like defense response, leaf development, root growth, flower development, embyronal develop- ment, hypocotyls growth, stomatal ...
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Chitinase activities, scab resistance, mycorrhization rates and biomass of own-rooted and grafted transgenic apple
... Previous reports have shown that AMF root coloniza- tion rates were not adversely influenced by overexpressing plant chitinase genes in tobacco (Vierheilig et al., 1993, 1995) and tomato (Girlanda et al., 2008). The ...
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Padronização de um paradigma comportamental de esquiva inibitória para Zebrafish (Danio rerio Hamilton, 1822)
... teleósteo zebrafish é um excelente modelo animal para estudos genéticos e de desenvolvimento e vêm mostrando enorme potencial para estudos de aprendizagem e ...do zebrafish segue padrões típicos dos ...do ...
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Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1.
... Methodology/Principal Findings: By laser microdissection cancer cell populations were harvested and subjected to whole genome expression analyses. Overall 473 and 541 genes were significantly regulated, when cancer ...
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Zebrafish transgenic line huORFZ is an effective living bioindicator for detecting environmental toxicants.
... a zebrafish transgenic line huORFZ, which harbors a GFP transgene regulated by the upstream open reading frame (uORF) fragment of human DNA-damage-inducible transcript 3 (ddit3, previously named chop) cDNA ...
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Telomerase Is Required for Zebrafish Lifespan
... tert 2/2 zebrafish using the S-phase marker PCNA revealed an overall decrease in cell division in proliferative tissues (Figure 4A– 4C, panels a–e). This was particularly clear in the spermatogenic zone of the ...
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Transgenic Passionfruit Expressing RNA Derived from Cowpea aphid-borne mosaic virus Is Resistant to Passionfruit Woodiness Disease
... with transgenic plants which accumulate the transgenic mRNA at low or non-detectable ...in transgenic plants when the introduction of a second copy of an endogenous gene led to the inactivation ...
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A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants.
... and transgenic tobacco plants and confirmed that AM79 aroA showed a higher glyphosate tolerance than that conferred by the other four aroA genes, indicating that AM79 aroA is a good candidate for the development ...
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