[PDF] Top 20 Bovine dedifferentiated adipose tissue (DFAT) cells

... fat cells (DFAT cells) are derived from lipid-containing (mature) adipocytes, which possess the ability to symmetrically or asymmetrically proliferate, replicate, and ...DFAT cells from any (one) ... See full document

... °C. Cells were then washed 2 times with PBS. Before sur- face staining, cells were incubated with 100 µg/mL of purified bovine IgG (Sigma-Aldrich) in Dulbecco’s PBS, 2% FBS, 2 mM EDTA, 10 mM HEPES as ... See full document

... (Miltenyi). Cells were cultured in Dulbecco’s modified Eagle medium (DMEM):F12 (Gibco) 50% heat-inactivated fetal calf serum (FCS; Gibco) and then in 20% serum for differ- entiation as described [ 53 ...μg/ml ... See full document

... obtained adipose tissue samples were washed with PBS in order to receive physical and chemical dissociation processes with the use of scalpel blades into a Petri dish containing 5ml of type I collagenase ... See full document

... stem cells under good manufacturing practice (GMP) conditions (RNL Bio, Seoul) and used as ‘anonymized’ ...the cells was not required for the ...fetal bovine serum (FBS; Invitrogen, Carlsbad, CA) and ... See full document

... subcutaneous adipose tissue was collected under sterile conditions from ten-week-old male Lewis rats and rinsed with phosphate-buffered saline ...the cells are homogeneous and express the surface ... See full document

... Collection of biological samples. Twenty-four hours, 7 and 21 days and 12 months after infection, mice were isoflurane anesthetized for retro-orbital blood collection and euthanized by cervical dislocation. For flow ... See full document

... and adipose tissue–derived stromal vascular fraction cells in two different cell culture ...the adipose tissue of the gluteal region near the base of the ...fetal bovine serum or ... See full document

... and bovine fibroblasts, OCT4 protein expression was reported (Rodríguez-Álvarez et ...fibroblasts cells, one explanation for this finding is the presence of OCT4 pseudogenes in bovine cells ... See full document

... The lipoaspirate tissue (2mL) was washed with 2 mL of phosphate-buffered saline (PBS; Sigma Aldrich, St. Louis, Missouri, USA) and incubated with 200 U/mL collagenase II for 60 min at 37ºC. After incubation, the ... See full document

... human adipose tissue was obtained from patients undergoing ...liposuction. Tissue samples were minced and digested in a solution of ...Foetal Bovine Serum (FBS) (Lonza, Basel, Switzerland) and ... See full document

... of adipose tissue from SUB and OM were transferred to two Petri ...fetal bovine serum) was added to the solution at a ratio of two parts of medium to one part of collagenase to neutralize the ... See full document

... culture, cells were stained with Alizarin Red S (Alfa Aesar, UK) which stains the mineralized matrix ...fetal bovine serum; 1% ITS + Premix (BD, Franklin Lakes, NJ, USA); 1µM dexamethasone (Alfa Aesar, UK); ... See full document

... marrow cells were collected with a Komiyashiki® ...method. Cells were cultured at a density of approximately 10 × 10 3 cells/cm 2 in 25-cm 2 flasks (Sarstedt, Newton, North Carolina, USA) containing ... See full document

... The adipose tissue can be collected from subcutaneous (superficial and deep) and visceral depots and from many internal organs ...the cells from omental and perineum as compared to the cells ... See full document

... mononuclear cells of rapamycin treated transplanted patients (Di Paolo et ...other cells can disrupt the mTORC2 and thereby decrease phosphorylation of PKB at Ser473 (Hagan et ...sc adipose ... See full document

... of adipose tissue agreed with a previous report [38] that showed increased IL-10 and decreased TNF-a expression in the brain after ...influenced cells toward a Type 2 immune ... See full document

... the cells were grown at high conluence for approximately one month in αMEM medium containing 10% FBS (Gibco), 10-8 M dexamethasone (Sigma), 5 mg / mL ascorbic acid (Sigma) and 10 mM of β-glycerophosphate ... See full document

... white adipose tissue. Since the discovery of adipose triglyceride lipase (ATGL) in 2004, many studies were performed to investigate and char- acterize the actions of this lipase, as well as of other ... See full document

... kidney tissue of an untreated animal and (E) an ASC-treated ...kidney tissue from an ASC-treated animal and (G) an untreated ...kidney tissue values normalized to hypoxanthine ... See full document