[PDF] Top 20 Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis
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Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis
... the commercial assay (ELISAcom) were performed according to the manufacturer’s instructions (Pourquier ...The in-house ELISA protocol (ELISAinh) was performed as previously described ... See full document
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Commercial enzyme-linked immunosorbent assay versus polymerase chain reaction for the diagnosis of chronic Chagas disease: a systematic review and meta-analysis
... one of the following: (i) to esti- mate sensitivity or specificity of one or more ELISA or PCR tests for chronic Chagas disease, (ii) to estimate the accuracy of an ELISA or PCR test for ... See full document
21
Evaluation of a bovine pregnancy-associated glycoprotein enzyme-linked immunosorbent assay kit for serological diagnosis of pregnancy in sheep
... Pregnancy diagnosis is an important tool for farm ...pregnancy diagnosis in ewes. As an alternative, radioimmunoassay (RIA) allows accurate and early detection of ... See full document
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Production of leptospiral LipL32 antigen in Pichia pastoris and its use in an enzyme-linked immunosorbent assay
... 2005), and higher than reported for canine (Dey et al. 2004) and convalescent human sera (Flannery et ...rLipL32 in the ELISA. These samples were tested by the immunoblotting assay using ... See full document
4
An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses
... antibody and a recombinant ...that an ELISA using recombinant EMA-1 expressed in insect cells by baculovirus can be a useful diagnostic reagent for detection of ...infection in horses ... See full document
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Use of heterologous antigens for the immunodiagnosis of abdominal angiostrongyliasis by an enzyme-linked immunosorbent assay
... immunodiagnosis of angiostrongyliasis since 1997 and later in the diagnosis of acute angiostrongyliasis (Graeff-Teixeira et ...1977) and latex ag- glutination using crude ... See full document
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The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys
... lands of these areas that favor the mechanical transmis- sion by tabanids and other haematophagous ...insects. In the Pantanal of Mato Grosso do Sul an endemic situation for ...first ... See full document
7
Efficacy of an enzyme-linked immunosorbent assay as a diagnostic tool for schistosomiasis mansoni in individuals with low worm burden
... is an immunoenzymatic method useful for detection of IgM antibodies against a fraction of Schisto- soma mansoni adult worm antigen (AWA) that is soluble in trichloroacetic acid ...groups ... See full document
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Evaluation of the dot enzyme-linked immunosorbent assay in comparison with standard ELISA for the immunodiagnosis of human toxocariasis
... Diagnosis of toxocariasis is based on clinical and se- rological data because of the difficulty in detecting larvae from ...agnosis of toxocariasis is ELISA using ... See full document
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Comparison of agar gel immunodiffusion test, enzyme-linked immunosorbent assay and PCR in diagnostics of enzootic bovine leukosis
... infection in cattle. Once infected, cattle remain virus carriers for life and start to show an antibody response within a few weeks after ...Eradication and control of the disease are ... See full document
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Research paper Combined use of Paracoccidioides brasiliensis recombinant rPb27 and rPb40 antigens in an enzyme-linked immunosorbent assay for immunodiagnosis of paracoccidioidomycosis
... one of the most important endemic mycoses in Latin America; it's usually diagnosed by observation and/or isolation of the etiologic agent, Paracoccidioides brasiliensis, as well as by a ... See full document
7
Quantification of urinary 5-hydroxyindoleacetic acid by in-house nitrosonaphthol reaction compared with nitrosonaphthol micro column chromatography and enzyme-linked immunosorbent assay
... aim of this study was to compare the colorimetric “kit” and enzyme-linked immunosorbent assay (ELISA) methods to quantify urinary 5-hydroxyindoleacetic acid through the ... See full document
5
Detection of Entamoeba histolytica/Entamoeba dispar in stool specimens by using enzyme-linked immunosorbent assay
... al. and examined by ...by an en- zyme immunoassay ...is an in vitro diagnosis intended for the detection of ...dispar in fecal speci- ...surface of the microtiter ... See full document
4
Evaluation of the double agar gel immunodiffusion test and of the enzyme-linked immunosorbent assay in the diagnosis and follow-up of patients with chronic pulmonary aspergillosis
... sensitivity, and the ELISA tests present higher sensitivity but an elevated percentage of cross-reactions with paracoccidioidomycosis and ...usually an underlying disease for chronic ... See full document
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Detection of the acute phase of abdominal angiostrongyliasis with a parasite-specific IgG enzyme linked immunosorbent assay
... panel of positive control sera collected at dif- ferent time points after surgery made the present evaluation of an ELISA system for diagnosis of the acute phase of infection ... See full document
4
Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis
... results of this investigation showed that ELISA using crude and recombinant antigens gave results to similar degrees of sensitivity and specificity in identifying different clinical ... See full document
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Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis
... results of this investigation showed that ELISA using crude and recombinant antigens gave results to similar degrees of sensitivity and specificity in identifying different clinical ... See full document
8
Detection of Giardia duodenalis antigen in human fecal eluates by enzyme-linked immunosorbent assay using polyclonal antibodies
... developed and standardized an enzime-linked immunosorbent assay (ELISA) to detect Giardia antigen in feces using rabbit polyclonal ...sucrose and percoll ...antigens ... See full document
4
The use of enzyme-linked immunosorbent assay and immunoblotting for the detection of Campylobacter fetus immunoglobulins in the cervico-vaginal mucus of female cattle
... use of immunoblotting in cervico-vaginal mucus samples for the diagnosis of bovine genital campylobacte- riosis in herds with no history of the disease could be a promissing tool ... See full document
8
Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting
... biotinylated an- ti-human immunoglobulin G (1:2,000 dilution) (Amer- sham, GE Healthcare Life Sciences, Buckinghamshire, UK) in PBS-BSA and 50 µL streptavidin-horseradish peroxidase conjugate ... See full document
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