[PDF] Top 20 Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle

... preparations of intracel- lular parasites are difficult to produce, particularly due to the presence of host contaminant compo- nents, such as red blood cell ...standardization of ... See full document

... distribution of Trypanosoma vivax in the Brazilian territory is expanding with potential to reach other areas, where the vectors are ...The detection of anti-trypanosomal ... See full document

... grown to approximately 30% confluence in culture plate wells, the culture supernatants were screened using the coating antigen hapten 1-OVA for the presence of antibodies that could recognize ... See full document

... according to the nucleotide sequence of M41 IBV (GenBank accession number ...inserted in the vector ...grown in synthetic complete medium without uracil to an optical density at ... See full document

... objectives of this work were to determine the prevalence of Babesia bovis, Babesia bigemina, and Anaplasma marginale detecting antibodies in cattle raised ... See full document

... Brasil IgM-ELISA is an immunoenzymatic method useful for detection of IgM antibodies against a fraction of Schisto- soma mansoni adult worm antigen (AWA) that is soluble ... See full document

... incidence of transplacental transmission of Anaplasma marginale in chronically infected cows with no history of acute anaplasmosis during ...gestation. In addition, we evaluated various ... See full document

... important in accomplishing these t ...developed to increase sensitivity and specifi- city (Wright ...used in enzyme- linked immunosorbent assay (ELISA) (Goff et ...but ... See full document

... observed in the elec- trophoresis profile of the three subspecies studied ...present in the tested vaginal mucus when the antigen produced with a sample of ...sample of C. fetus subsp. ... See full document

... diagnosis of trypanosomosis in animals with low parasitaemia is hampered by low diagnostic sensitivity of traditional detection ...methods. An immunodiagnostic method based on a direct ... See full document

... or in combination with hybridization have been used for detecting VL in humans and dogs (Berrahal et ...ability to detect and identify the protozoa involved and the ability to be applied ... See full document

... problem in Brazil. In Rio de Janeiro State, PFEIFER-BARBOSA et ...detected antibodies anti-B. equi in 100% of the serum samples analyzed by IFAT and 96,7% by ...prevalence of 73% ... See full document

... morbidity in cattle, leading to economic ...objective of this study was to deter- mine, by indirect enzyme-linked immunosorbent assay (ELISA), the frequency ... See full document

... revived in PLT cells. Bulk production of the ref- erence virus was carried out in confluent PLT cell culture flasks (300 ...reconstituted in 1 ml of ster- ile distilled ...grown ... See full document

... study, an enzyme-linked immunosorbent assay (ELISA) standardized with vesicular fluid of Taenia solium cysticerci was used to screen for IgG (total and subclasses) and IgE ... See full document

... values of IgG subclasses reactive against P. falciparum CSP appeared to be age- dependent and ranged from ...IgG4 in younger children to 0.95 for IgG3 in older ...investigated in ... See full document

... gradient in TNE ...g in a Sorvall AH-629 ...run of gradients were pooled, diluted with TNE buffer, and ultracentrifuged at 4°C for 4 h at 96,000 3 g in a Sorvall AH-629 rotor for sucrose ... See full document

... subclasses of immunoglobulins involved in toxocariasis (KAYES et ...the detection of total IgG anti-Toxocara, which is currently the standard serological method for the diagnosis of ... See full document

... Similar to our observation Rajesh et al. [23] in Kerala also reported higher prevalence in unorga- nized herds ...Contrary to the present findings, Singh and Yadav [5] observed significantly ... See full document

... the enzyme- linked immunosorbent assay (ELISA) has been very useful and popular since it was introduced to plant virology by Clark and Adams ...used in a wide range of ... See full document