[PDF] Top 20 A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples
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A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples
... amplification of mycobacterial DNA in clinical samples. Use of polymerase chain reaction for rapid diagnosis of tubercu- losis. Detection of Mycobacterium tuberculosis in spu- tum sample[r] ... See full document
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Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
... and clinical isolates - To standardize and evaluate the CRDH, we used a panel of 156 DNAs ob- tained from ...M. tuberculosis cultures that had been sub- mitted to both AST and partial rpoB gene ... See full document
7
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR
... Analysis of serial dilutions of DNA from reference strains of ...tivity in nested-PCR than in real-time PCR alone or con- ventional PCR, although this difference ... See full document
8
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR
... agent of bovine ...as PCR can provide fast and reliable results while significantly decreasing the time of ...confirmation. In the present study, a nested-PCR system, targeting ... See full document
8
Reliable single cell array CGH for clinical samples.
... performance of PCR-T2 with the recently published RP labeling procedure ...cells of OE-19 esophageal cancer cells. Irrespectively of the labeling approach used, two aberrations of only ... See full document
10
PCR identification of Mycobacterium tuberculosis complex in a clinical sample from a patient with symptoms of tuberculous spondylodiscitis
... are more frequently ...and Mycobacterium tuberculosis, but many other microorganisms have also been shown to be associated with this condition ... See full document
4
Fluorescent quantitative PCR of Mycobacterium tuberculosis for differentiating intestinal tuberculosis from Crohn’s disease
... most reliable differentiation method is finding evidence of MTB in intestinal ...sensitivity. In addition, the biopsy culture for MTB is time consuming (3-8 weeks), and the results are ... See full document
5
Low-stringency single-specific-primer PCR as a tool for detection of mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis
... economies of developing countries since the disease generally strikes in- dividuals in their prime working ...Resistance of Mycobac- terium tuberculosis to antituberculosis drugs is a ... See full document
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REAL-TIME PCR DETECTION OF LISTERIA MONOCYTOGENES IN FOOD SAMPLES OF ANIMAL ORIGIN
... level of detection was ...amplification in all replicates) with the CT value of ...real-time PCR assay we developed was approximately 1000-fold more sensitive than conventional ... See full document
9
Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens
... a reliable and rapid assay that could be easily incorporated into a clinical labora- tory ...for detection of M. tuberculosis, culture, and microscopy are undoubtedly much cheaper than ... See full document
6
Culture-independent detection and characterisation of Mycobacterium tuberculosis and M. africanum in sputum samples using shotgun metagenomics on a benchtop sequencer
... availability of rapid, cheap high-throughput sequencing and, particularly, the arrival of user-friendly benchtop sequencing platforms, such as the Illumina MiSeq (Loman et ...use of whole-genome ... See full document
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A Multiplex real-time PCR for detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from Brazilian commercial poultry flocks
... identification of the bacteria is still con- sidered the “gold standard” for diagnosis of Mycoplasma infections (Kleven, 2008b; Nascimento and Pereira, ...development of other commensal bacteria ... See full document
6
Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis
... goal of diagnos- ing the bacillus M. tuberculosis in bacterial suspension ...number of positive and negative samples shows that it provides repeatability, reproduci- bility and ... See full document
7
Evaluation of rapid alternative methods for drug susceptibility testing in clinical isolates of Mycobacterium tuberculosis
... performance of a commercial method (MGIT) and four inexpensive drug susceptibility methods: nitrate reductase assay (NRA), microscopic observation drug susceptibility (MODS) assay, MTT test, and broth ... See full document
8
Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection
... (1990). PCR was performed in a final volume of 50 µL, containing ...each of dATP, dCTP, dGTP and dTTP, 10 mM Tris-HCl (pH ...pmoles of each primer (INS-1 and INS-2) and 2.5 units ... See full document
6
Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
... advantages of using the pH assisted method for probe synthesis were not confirmed and consequently all probes were synthesised using the salt-aging ...simultaneous PCR amplification of the two loci ... See full document
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EVALUATION OF METHODS FOR DETECTION AND IDENTIFICATION OF MYCOBACTERIUM SPECIES IN PATIENTS SUSPECTED OF HAVING PULMONARY TUBERCULOSIS
... Ministry of Health policies in Brazil. In the present work, the detection of Mycobacterium tuberculosis by the Polymerase Chain Reaction (PCR) was standardized, and ... See full document
6
Rapid and accurate detection of Mycobacterium tuberculosis in sputum samples by Cepheid Xpert MTB/RIF assay--a clinical validation study.
... and in alignment with the GRADE approach to guideline development [8]. In 2009, the first technical data were announced from an automated molecular test for TB, the Xpert MTB/RIF Assay co-developed by the ... See full document
8
Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
... use of PCR has been hindered by problems such as contamination and inhibition of the reaction ...Use of PCR for rapid diagnosis of tuberculosis in a developing ... See full document
4
Effects of tissue handling and processing steps on PCR for detection of Mycobacterium tuberculosis in formalin-fixed paraffin-embedded samples
... de Mycobacterium tuberculosis em amostras clínicas é um objetivo importante nos laboratórios de todo o ...a PCR para amplificação do gene da beta- actina humana e, separadamente, para amplificação da ... See full document
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