[PDF] Top 20 Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method

... (outer and inner primers, F3/B3 and FIP/BIP, respectively) targeting the speci fic gene were designed by the Primer Explorer V5 software (http: ...assay. The outer primers ... See full document

... more sensitive than that of conventional ...detected by agarose gel electrophoresis, but also visually. The LAMP assay is a rapid, simple, specific, sensitive and ... See full document

... detected by agarose gel electropho- resis, by the use of spectrophotometric equipment to measure turbidity [13], or by visual inspection of turbidity or color changes ... See full document

... with the IRB approval. The DNA was also extracted by boiling method(Aziah et ...2007). The LAMP assay was carried out twice for each sam- ple. To confirm the specificity ... See full document

... for the detection of Histoplasma capsulatum are needed in regions with limited resources in which the or- ganism is endemic, where delayed diagnosis of progressive ... See full document

... endemic in tropical Africa. In eastern and southern Africa the disease is caused by Trypanosoma brucei rhodesiense, while ...common in central and West ...form of ... See full document

... combines the capacity of PMCA technology to propagate PrP TSE and infectivity in vitro [20, 21], with prion-contaminated steel wires as the source of seeding infectious ...that ... See full document

... become the most widespread zoonosis in the world ...lacking of typical clinical features. Treatment of Q fever with antibiotics in the acute phase is effective, but once ... See full document

... chiseptica and other respiratory pathogens results in more severe disease than infection with ...2008). In pigs, B. bronchiseptica is a primary etiologic agent of swine atrophic rhinitis ... See full document

... limitation of these methods is their lack in sensitivity, especially in low-grade infections, as occurs in areas of low prevalence or in individuals with recent infections ... See full document

... h detection period. In addition, our method is highly specific as the enhancement of the sensitivity is due to the better preservation of the target ... See full document

... studies and biochemical assays have been developed for detection and identification of ...effectiveness and accuracy, these assays are time consuming, usually requiring up to 5 days to ... See full document

... Loop-mediated isothermal amplification (LAMP) is a simple, rapid, sensitive, and specific method for the detection of pathogens that was ... See full document

... Recently, the use of pharmacological chaper- ones has also been suggested as a treatment strategy for MPS IIIB ...[69]. In general, the modulation of the proteostasis network is ... See full document

... set of four primers (B3, F3, BIP and FIP), recognizing six distinct sequences within a target DNA in total (Fig ...groups of primers were designed and filtered to select the best ... See full document

... Loop-mediated isothermal amplification (LAMP) assay is a rapid and specific DNA amplification method described originally by Notomi et ...set ... See full document

... The loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that has been developed re- cently (Notomi et al ...performed by an ... See full document

... one of the most dramatic medical emergencies, which is seen as a public health challenge ...world-wide. The disease is dreaded by parents and health care workers alike for its acute on- ... See full document

... All the laboratory assays were performed at the Molecular Epidemiology and Infectious Diseases Laboratory (LEMDI) of the Faculty of Medical Sciences, University of ... See full document

... ﻖﯿﻗد و ﻊﯾﺮﺳ ﯽﻫﺎﮕﺸﯾﺎﻣزآ ﺺﯿﺨﺸﺗ ﺖﻬﺟ لﺪﻣ ﮏﯾ ناﻮﻨﻋ ﻪﺑ ﯽﻋﻮﻨﺼﻣ هﺪﺷ هدﻮﻟآ مﺮﺳ يﺎﻫ ﻪﻧﻮﻤﻧ رد.. دﻮﺑ ﯽﻧﺎﺴﻧا زﻮﻠﺳوﺮﺑ. رﺎﮐ شور.[r] ... See full document