[PDF] Top 20 Vitrification of oocytes and in vitro produced bovine embryos exposed to cytochalasin B
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Vitrification of oocytes and in vitro produced bovine embryos exposed to cytochalasin B
... Após 22 h de maturação, os oócitos foram parcialmente desnudados e, de forma aleatória, submetidos aos diferentes tratamentos. No tratamento 1, os oócitos foram submetidos diretamente a vitrificação (Tratamento Vitri). ... See full document
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The effect of interaction between macromolecule supplement and oxygen tension on bovine oocytes and embryos cultured in vitro
... aiming to standardize IVP procedures and also to avoid pathogens transmission, synthetic and defined supplement sources are frequently used, including polyvinyl alcohol (PVA; Fukui et ...1999) ... See full document
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Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos
... research in serum supplementation during in vitro culture have focused on developmental rates and gene expression [for review see (Lonergan et ...cations in embryos primarily ... See full document
8
In vitro production of buffalo embryos from stepwise vitrified immature oocytes
... freeze and vitrification, have been used to preserve embryos and oocytes of many animal species, resulting in the birth of live ...offspring. ... See full document
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Effect of supplementation of green tea polyphenols on the developmental competence of bovine oocytes in vitro
... here in Experiment 3 indicate that the proportion of oocytes developing to the blastocyst stage was sig- nificantly higher (P < ...0.05) in the presence of a low GTPs ... See full document
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Joana Claudia Mezzalira1 Silvério Bunn1 Fabiano Buss Cruz1 Arnaldo Diniz Vieira1 Alceu Mezzalira1 Nitrogênio super resfriado por vácuo melhora a capacidade de desenvolvimento de oócitos bovinos após vitrificação
... objective of this study was to determine the effects of vacuum-cooled liquid nitrogen on the development of vitrified immature (germinal vesicle stage; GV) and mature (metaphase II; ... See full document
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Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro
... quality of blastocysts was evaluated by the inner cell mass (ICM) and trophectoderm (TE) cell ...identification of ICM and TE, for subsequent quantification, was based on the modified technique ... See full document
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Sabine Wohlres-Viana1 , Mariana Cortes Boite2 , João Henrique Moreira Viana3 , Marco Antonio Machado 1,3 , Luiz Sérgio de Almeida Camargo
... lack of differences between in vivo and in vitro produced embryos suggests that the level of AQP 11 transcripts is not influenced by the culture ...system. ... See full document
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Effects of heat stress on development, quality and survival of Bos indicus and Bos taurus embryos produced in vitro
... days of the estrous cycle, cumulus-oocyte complexes (COCs) were collected using transvaginal ultrasound-guided oocyte collection with a 5-MHz sectorial transducer equipped with a needle guide and attached ... See full document
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Oocyte activation and preimplantation development of bovine embryos obtained by specific inhibition of cyclin-dependent kinases
... efficiency of bohemine and roscovitine in combination with ionomycin on parthenogenetic activation and initial embryonic development of bovine oocytes was ...performed: ... See full document
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Evaluation of developmental changes in bovine in vitro produced embryos following exposure to bovine Herpesvirus type 5
... According to a previous study, the blastocyst hatch is characterized by a mito- chondrial distribution pattern changing from perinuclear to pericytoplasmatic localization, while in the cells ... See full document
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The effects of ovalbumin as a protein source during the in vitro production of bovine embryos
... 32 to 36 h post-insemination under a stereomicroscope (magnification of 50 ...presence of two-to- four-cell ...number of treated oocytes, and hatching rate was based on ... See full document
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Lipid content and cryotolerance of in vitro-produced bovine embryos treated with forskolin before vitrification
... addition of FCS in the culture medium is one of the main factors responsible for causing morphological and physiological differences (Abe et ...2008) in IVC embryos when compared ... See full document
6
Supplementation with the histone deacetylase inhibitor trichostatin A during in vitro culture of bovine embryos
... result in the increased expression of specific ...beneficial and harmful genes are stimulated and their in- creased expression activates different pathways that improve or worsen ... See full document
5
Vitrification of bovine preantral follicles with dimethylsulfoxide and sucrose plus α-tocopherol
... used in combina- tion, with the possibility of optimizing ...dimethylsulfoxide, and can be combined with in- tra or extracellular cryoprotectant as sucrose in protocols of female ... See full document
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Polyadenylated tail length variation pattern in ultra-rapid vitrified bovine oocytes
... mRNA and protein synthesized and stored during ...involved in development after the so-called mater- nal-embryonic transition since it has been shown in mice that protein synthesized from ... See full document
5
Evaluation of susceptibility of the ZRE1 alloy to hot cracking in conditions of forced strain
... order to evaluate the susceptibility to hot cracking in the high-temperature brittleness range, we have determined the changes of temperature of individual points when the alloy was ... See full document
6
Effect of concentration and exposure period to butyrolactone I on meiosis progression in bovine oocytes
... made in bovine (Lagutina et al., 2002) and pig oocytes (Marchal et ...2001). In pig oocytes, a greater acceleration in maturation kinetics was observed when the culture ... See full document
6
Bovine conceptus of Bos indicus produced by somatic cell nuclear transfer and parthenogenesis present morphological variations since the blastocyst stage
... SCNT embryos, microvilli were present, particularly in surface trophectoderm cells in contact with the zona pellucida ...forms of cellular communication were gap junctions between cells ... See full document
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Embryos refrozen-thawed by vitrification lead to live births: Case report
... cryopreservation of embryos by the slow-freezing meth- od continues to be standard practice (3) mostly due to the quantity of information and experience in comparison ... See full document
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