A realização deste trabalho permitiu determinar várias características físico-químicas da lacase purificada de P. rufa no entanto também seria interessante futuramente proceder à determinação de:
- Ponto isoelétrico.
- Ião cobre por colorimetria e por espectrometria de absorção atómica. - Potenciais redox.
- Influência do pH com outro substrato como por exemplo guaiacol. - Espectros de absorção.
- Estudo com várias concentrações de inibidores.
-Estudo com diferentes inibidores e substratos de modo a caracterizar o mecanismo de reacção.
- O tipo de inibição da lacase estudando outros modelos cinéticos não lineares. - Purificação do pico obtido ao fim de 23 dias.
Considerando o fungo P. rufa seria interessante proceder a purificação e caracterização da MnP e LiP. Assim como utilizar outros indutores que permitam estimular a segregação da lacase tendo em vista a sua purificação e caracterização.
Outro estudo interessante a ser feito seria testar a produção de lacase com este fungo noutros meios de cultura baratos e acessíveis.
Uma vez que este fungo produz uma elevada actividade de lacase seria importante imobilizar esta enzima em diferentes suportes de forma a estudar a influência desta técnica na actividade, estabilidade da enzima, especificidade e inibição.
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