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Cobalt-60 Gamma Rays and IUdR on Cultured
Glioblastoma Spheroid Cells and Dosimetry
Using TLD-100
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*Corresponding Address: P.O.Box: 14155-6183, Department of Radiobiology, Iran University of Allied Health, Hemat Highway, Tehran, Iran
Email: neshastehriz@yahoo.com
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Abstract
2EMHFWLYHIn radiation treatment, the irradiation which is effective enough to control the tumors far exceeds normal-tissues tolerance. Thus to avoid such unfavourable outcomes, some methods sensitizing the tumor cells to radiation are used. Iododeoxyuridine (IUdR) is a halogenated thymidine analogue that known to be effective as a radiosensitizer in
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to hyperthermia depends on the magnitude of the differential sensitization of the hyper-thermic effects or on the differential cytotoxicity of the radiation effects on the tumor cells. In this study, we evaluated the combined effects of IUdR, hyperthermia and gamma rays of 60Co on human glioblastoma spheroids culture.
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respectively. The DNA damages induced in cells were compared using alkaline comet assay method, and dosimetry was then performed by TLD-100. Comet scores were calcu-lated as mean ± standard error of mean (SEM) using one-way ANOVA.
5HVXOWVComparison of DNA damages induced by IUdR and hyperthermia + gamma treat-ment showed 2.67- and 1.92-fold enhancetreat-ment, respectively, as compared to the dam-ages induced by radiation alone or radiation combined IUdR. Dosimetry results showed the accurate dose delivered to cells.
&RQFOXVLRQAnalysis of the comet tail moments of spheroids showed that the radiation
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when compared to related results of irradiation alone or of irradiation with IUdR. These re-sults suggest a potential clinical advantage of combining radiation with hyperthermia and indicate effectiveness of hyperthermia treatment in inducing cytotoxicity of tumor cells.
Keywords: Radiation, Hyperthermia, IUdR, Glioblastoma, Comet Assay
Cell Journal(Yakhteh), Vol 16, No 3, Autumn 2014, Pages: 335- 342
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Introduction
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According to the small number of patients cured
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tumors are basically resistant to common cancer
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which competes with thymidine for incorporation
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ter radiation treatment. These substances increase
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Hyperthermia or thermotherapy (thermalthera
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ment is always used with other methods such as
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and radiation therapy or chemotherapy has often
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stretch out the ends of damaged molecules and cre
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To assess the effect of hyperthermia alone and in combination with radiation on human glioblas
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The multicellular spheroid is a transition model of in vitroin vivo which is important for in vivo
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differences between cell growth in two dimen
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Two researches conducted on the growth of human
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higher radioresistance of cells in spheroids as
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bination with hyperthermia treatment before irra diation by gamma rays. Also for determining the accuracy of radiation absorbed dose in cellular un
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Materials and Methods
Cell line
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Monolayer culture
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Spheroid Culture
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Spheroid Growth curve
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ume was calculated using the following equation:
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calculated according to the duration. In the logarith
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Drug treatment
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Hyperthermia and Irradiation
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assay. This assay was performed according to the
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rescence intensity using the comet score software.
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TL Dosimetry
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consists of heating the thermoluminescent dosimeters
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slab to establish the same conditions which cells were undergoing.
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Statistical analysis
The results of the comet score (tail moments)
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Results
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Table 1: Mean tail moment of cells was measured using comet score software and SE values of samples
Standard error (SE) Mean tall moment
Group
Control
IUdR
+\S
,8G5+\S
Gamma 60Co
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+\S*DPPD60Co
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IUdR; Iododeoxyuridine and Hyp; hyperthermia.
Representative samples include following slides $&RQWURO%,8G5&+\S',8G5+\S (*DPPD),8G5*DPPD*+\S*DPPD DQG+,8G5+\S*DPPD
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display an ascending amount in tail moments and number of damages from A to H.
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belonged to control group.
As shown in the ¿JXUHK\SHUWKHUPLDDORQH
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as compared with the control group. But the
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and hyperthermia.
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Fig 4: Comparative chart of comet tail moment in U87MG
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hour) and irradiated by gamma rays of cobalt-60. Mean ± SEM of the three separate experiments are plotted. Lines
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Discussion
Hyperthermia has been used in the therapy of
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cell membranes in such a way that normal cells
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the mechanisms of hyperthermic therapy for ma
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glioblastoma. Van Bree et al. Reported supposi tion about the role of hyperthermia in the treat ment of tumors based on the combined use of hy
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effects of radiation as compared to radiation ther apy alone. This means that hyperthermia can be
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ties in vitroWRGHWHUPLQHQRUPDOWLVVXHWR[LFLW\DQG
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of cells.
Conclusion
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multicell spheroids potentiated by hyperthermia.
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age.
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greater than when cells were irradiated without treatment.
These results show that a combination of hyper
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damages in multicell spheroids. This interaction
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es. The possibility of clinical implication of using three different modalities simultaneity depends on that all equipment used are aggregated in one place.
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reading. This uncertainty was found to be high but
7KHUHIRUHWKHDFFXUDF\RI7/'XVHGLQWKLV H[SHULPHQWVKRZVWKDWWKHDEVRUEHGGRVHE\FHOOV
is in accordance with irradiated dose and consid
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Acknowledgments
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interest in this study.
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