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Protein Degradation of RNA Polymerase II-Association Factor 1(PAF1) Is Controlled by CNOT4 and 26S Proteasome.

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Academic year: 2017

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Fig 1. PAF1 is regulated by CNOT4 at the protein level. (A, B) The levels of endogenous PAF1 protein in CNOT4-overexpressing or silenced whole-cell lysates were analyzed by immunoblot
Fig 2. CNOT4 regulates PAF1 but not other PAFc components. (A, B) Endogenous PAFc protein levels were measured in CNOT4-overexpressing or silenced HEK293 cells via Western blot
Fig 3. CNOT4 controls the degradation of PAF1 via the 26S proteasome. (A) Pulse-chase experiment
Fig 4. CNOT4 regulates the ubiquitination status of the PAF1 protein. (A) Physical interaction between PAF1 and CNOT4
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