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Identification of RegIV as a novel GLI1 target gene in human pancreatic cancer.

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Academic year: 2017

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Figure 1. Construction of the pLVTHM vector encoding anti-GLI1 shRNA. For the GLI1-shRNA vector, two single strand DNAs encoding two linkers, the target sequences and a loop element, were synthesized
Figure 3. Expression of GLI1 and RegIV proteins was analyzed by IHC in PC and adjacent normal tissues
Figure 7. Modulation of GLI1 binding on RegIV promoter was assessed by Chromatin immunoprecipitation (ChIP) assay

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